
Product Name | Bacterial Sortase Substrate II , Dabcyl/EdansDabcyl - QALPETGEE - Edans |
Size | 1 mg |
Catalog # | AS-62230 |
US$ | $272 |
Purity | % Peak Area By HPLC ≥ 95% |
This peptide is a C-terminal surface sorting signal with a conserved LPXTG motif, labeled with the Dabcyl/Edans FRET pair. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. Surface proteins of Staphylococcus aureus are anchored to the bacterial cell wall by a mechanism requiring this C-terminal sorting signal. Cell wall sorting is the covalent attachment of surface proteins to the peptidoglycan via a C-terminal sorting signal that contains a consensus LPXTG sequence. Cleavage of this FRET substrate by sortase reveals the fluorescent signal that may be measured to study sortase activity. Inhibition of the sortase activity is a potential way of treatment of the staphylococcal infection. The LPXTG motif is conserved in more than 100 surface proteins of Gram-positive pathogens. | |
Detailed Information | ![]() |
Storage | -20°C |
References | Ton-That, H. et al. PNAS 96, 12424 (1999); W. W Navarre and O. Schneewind Mol. Biol. Rev. 63, 174 (1999); Kang, S. et al. Biol. Pharm. Bulletin 29, 1751 (2006); Ilangovan, U. et al. PNAS 98, 6056 (2001). |
Molecular Weight | 1472.6 |
Dabcyl-QALPETGEE-Edans | |
Sequence(Three-Letter Code) | Dabcyl - Gln - Ala - Leu - Pro - Glu - Thr - Gly - Glu - Glu - Edans |
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适应症下会标注“本品适用于敏感菌所致......”字样;
药理作用下会标注“本品属......类抗菌素”字样。
2.β-内酰胺类青霉素(阿莫西林、氨苄西林、羧苄西林) 头孢菌素、碳青霉烯类、头霉素、单内酰环类、β-内酰酶抑制剂、甲氧青霉素类
3.大环内酯类 红霉素、白霉素(吉他霉素)、罗红霉素、阿奇霉素、克拉霉素、乙酰螺旋霉素、麦迪霉素、交沙霉素等
4 酰胺醇类:包括氯霉素、甲砜霉素等
5 四环素类:包括四环素、土霉素(强力霉素)、金霉素等
6 林可酰胺类 克林霉素、林可霉素
7.糖肽类抗生素: 比如万古霉素、替考拉宁
8 抗真菌抗生素:如灰黄霉素、两性霉素B
9 抗肿瘤抗生素:如丝裂霉素、放线菌素D、博莱霉素、阿霉素等
10 具有免疫抑制作用的抗生素如环孢霉素
11 多磷类抗生素:如磷霉素

