Type:RabbitIgG
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Wholeserum-lyophilized
Immunogen:Asyntheticpeptidesequencecorrespondingtoaminoacids384-398predictedfromtheclonedratMOR1.Thepeptidewasconjugatedtobovinethyroglobulinwithglutaraldehyde.
Pleasednotethisantibodywasgeneratedusingasyntheticpeptidesequencecorrespondingtoaminoacids384-398predictedfromtheclonedratMOR1.Thepeptidewasconjugatedtobovinethyroglobulinwithglutaraldehyde(MuOpioidReceptorCatalog#RA10104wasmadeusingapeptidecorrespondingtoaminoacids386-400(NHQLENLEAETAPLP).
Threetypesofopioidreceptorshavebeencloned--mu,delta,andkappa.OpioidreceptorsareseventransmembraneG-proteincoupledreceptors.TheyshareahighdegreeofhomologyandaremostdivergentattheN-andC-termini.ActivationofmuopioidreceptorsleadstoadecreaseinneuronalexcitABIlity.
Seedoi:10.1016/j.neuroscience.2006.10.033forIHC/WBimagesanddata.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
细胞质中有各种细胞器,细胞内的化学反应大多都是在细胞质中进行,因此是代谢中心.
细胞核中有DNA,是遗传信息库,是细胞代谢和遗传的控制中心.
解析:1.细胞质基质(也叫胞质溶胶)是指除细胞器外细胞质的其余部分。细胞质基质是活细胞进行新陈代谢的主要场所。
2.细胞新陈代谢的次要场所是:细胞核、线粒体基质、叶绿体基质等基质。
不破坏细胞结构,同步动态侦测细胞有氧呼吸,糖酵解OCR/ECA(总或乳酸ECA);
i.过氧敏感荧光素(或pH敏感荧光素),Ex340-380/535/Em630-680nm,实时测量线粒体/胞内/胞外重要代谢指标,荧光素为非结构性结合可逆转改变;
ii.可同时或单个测量OCR/ECA或其他参数,不会做成浪费,可采用时间分辨荧光技术可加强系统信噪比
iii.通过两个加药口,可对检测细胞加入适当抑制剂/刺激实时监测细胞对不同毒素作用