
- Description
- Additional Information
- Readable Documents
- Assay Principle
- Reviews
Introduction
Sarcosine is natural amino acid that is an important intermediate in the metabolism of choline. Sarcosine is an important component of proteins and plays a significant role in metabolic processes of living cells as a source of serine, creatine, purines and glutathione etc. Sarcosine is present in food sources like legumes, eggs, ham, turkey etc. It is used in a variety of industrial applications such as manufacturing of tooth paste and biodegradable surfactants. Sarcosine was recently reported to activate prostate cancer cells and as a possible marker for prostate cancer progression to metastasis(1). It is also used in adjunctive treatment for Schizophrenia (2) and depression (3).
Cell Technology’s Fluoro Sarcosine assay provides a reliable, sensitive fluorimetric method for the quantification of sarcosine in biological samples.The Fluoro Sarcosine detection kit utilizes a non-fluorescent detection reagent, which is reduced via an enzyme-coupled reaction in the presence of sarcosine. A sarcosine standard curve is generated to quantify sarcosine in the samples.
Key Benefits
- Easy to Use – No Wash Assay.
- Detection of Sarcosine in cells, serum, tissue extracts.
- Easy to Use 96-well Fluorescent Plate reader readout.
Additional information
Kit Size | 100 |
---|
Document Title |
Fluoro Sarcosine Protocol |
Fluoro Sarcosine Datasheet |
msds.fluoroSarcosine |
Reference |
Sreekumar, Arun; Poisson, Laila M.; Rajendiran, Thekkelnaycke M.; Khan, Amjad P.; Cao, Qi; Yu, Jindan; Laxman, Bharathi; Mehra, Rohit et al. (2009). "Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression". Nature 457 (7231): 910. doi:10.1038/nature07762. PMID 19212411. |
Lane H, Huang C, Wu P, Liu Y, Chang Y, Lin P, Chen P, Tsai G (2006). "Glycine transporter I inhibitor, N-methylglycine (sarcosine), added to clozapine for the treatment of schizophrenia". Biol Psychiatry 60 (6): 645–9. doi:10.1016/j.biopsych.2006.04.005. PMID 16780811. |
images/CellTechnology/NCT00977353 Clinicaltrials.gov "N-methylglycine (Sarcosine) Treatment for Depression". |
Part# | Reagent | Temperature |
Part # 6024 | Enzyme Mix, 1 Vial | -20C |
Part # 3056 | Standard Curve Diluent, 40mL | 2-8C |
Part # 4023 | Sarcosine Detection Reagent, 1 Vial | 2-8C |
Part # 7021 | Sarcosine Standard, 1 Vial | 2-8C |
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Thy-1、c-kit散在分布于HB组织中,主要集中在汇管区,而正常肝脏组织中不表达;CD34及SCF在HB中的表达明显高于正常肝脏组织(P0.05),其中CD34主要在血管内皮系统中分布,SCF主要表达在汇管区;CD56表达于成簇的神经纤维组织中,在HB及正常肝脏组织中表达差异无统计学意义(P0.05)。结论不同干细胞相关表面标志物分布于HB组织中,并且集中表达在特定区域。表达Thy-1/c-kit阳性细胞可能对HB的发生起一定作用。
1,逆转录病毒将GFP基因导入干细胞,这种办法在导入基因时好像比较麻烦,但是检测好像比较简单。
2,将雄性干细胞植入雌性动物,追踪Y染色体,即SRY基因,但是看文献上说SRY追踪的FISH技术复杂,费用高,但是还没有看到具体的技术步骤,也不知道具体要多少费用。
请多指教
Thy-1、c-kit散在分布于HB组织中,主要集中在汇管区,而正常肝脏组织中不表达;CD34及SCF在HB中的表达明显高于正常肝脏组织(P0.05),其中CD34主要在血管内皮系统中分布,SCF主要表达在汇管区;CD56表达于成簇的神经纤维组织中,在HB及正常肝脏组织中表达差异无统计学意义(P0.05)。结论不同干细胞相关表面标志物分布于HB组织中,并且集中表达在特定区域。表达Thy-1/c-kit阳性细胞可能对HB的发生起一定作用。
有几个疑问
1:荧光标记到细胞是标记到细胞表面还是细胞质内?
2:荧光应该随着细胞的分化和增殖逐渐消失?是不是分化增殖越快,荧光消失速度越快?
3:有哪些容易操作,成本便宜的荧光物质?
谢谢各位战友

