
- Description
- Additional
Description
Details
Description:Rat monoclonal antibody to mouse cluster of differentiation 8 (CD8) antigen
Product Type:Primary antibody
Target Protein:Murine T-cell surface antigen CD8 marker
Immunogen:Mouse cytolytic T-lymphocytes
Specificity:Reactive with mouse T-cell surface antigen CD8 marker
Species Reactivity:Mouse, others not tested
Host / Isotype:Rat, IgM
Formulation:Lyophilized from a solution in 0.01M PBS pH7.2
Reconstitution:Double distillated water is recommended to adjust the final concentration to 1.00 mg/mL.
Storage: Store at -20oC
Research Area:Immunology
Background:
CD8 is a transmembrane glycoprotein receptor at the surface of cytotoxic T cell, natural killer cells and dendritic cells. CD8 binds major histocompatibility complex class I (MHC I) and arguments T cell activation by associating with T cell receptor. It plays an important role in the recognision and destruction of virally infected cells and tumour cells.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
Product Specificity | Rat anti-Mouse CD8, JXYT8 |
---|---|
Application | No |
Size | 0.1 mg |
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
Thy-1、c-kit散在分布于HB组织中,主要集中在汇管区,而正常肝脏组织中不表达;CD34及SCF在HB中的表达明显高于正常肝脏组织(P0.05),其中CD34主要在血管内皮系统中分布,SCF主要表达在汇管区;CD56表达于成簇的神经纤维组织中,在HB及正常肝脏组织中表达差异无统计学意义(P0.05)。结论不同干细胞相关表面标志物分布于HB组织中,并且集中表达在特定区域。表达Thy-1/c-kit阳性细胞可能对HB的发生起一定作用。
1,逆转录病毒将GFP基因导入干细胞,这种办法在导入基因时好像比较麻烦,但是检测好像比较简单。
2,将雄性干细胞植入雌性动物,追踪Y染色体,即SRY基因,但是看文献上说SRY追踪的FISH技术复杂,费用高,但是还没有看到具体的技术步骤,也不知道具体要多少费用。
请多指教
Thy-1、c-kit散在分布于HB组织中,主要集中在汇管区,而正常肝脏组织中不表达;CD34及SCF在HB中的表达明显高于正常肝脏组织(P0.05),其中CD34主要在血管内皮系统中分布,SCF主要表达在汇管区;CD56表达于成簇的神经纤维组织中,在HB及正常肝脏组织中表达差异无统计学意义(P0.05)。结论不同干细胞相关表面标志物分布于HB组织中,并且集中表达在特定区域。表达Thy-1/c-kit阳性细胞可能对HB的发生起一定作用。
有几个疑问
1:荧光标记到细胞是标记到细胞表面还是细胞质内?
2:荧光应该随着细胞的分化和增殖逐渐消失?是不是分化增殖越快,荧光消失速度越快?
3:有哪些容易操作,成本便宜的荧光物质?
谢谢各位战友

