- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function.
Symbols/Related Terms:
- COL1A1
- COL1A2
- osteogenesis imperfecta
Data/Specifications
Purity:
Rat collagen type I - 90%
Rat collagen type III - 10%
Other collagens - below 1%
Non-collagen proteins - below 0.5%
Form: 20 mg lyophilized, salt-free
Purification:Partial pepsin digestion in acidic conditions and differential salt precipitation.
Reconstitution:Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils.
Storage:Collagen dissolved in acetic acid is stable at 4 °C for 1 month. Lyophilized collagen long term storage (2 years) at -20°C or lower.
Literature/Support
Rat Type I Collagen Insert (PDF)
Note: inserts are for review only. Please refer to the insert shipped with your product to ensure the most up-to-date revision is being used.
How To Use
Key Applicatons:
- Used for rat collagen type I standard.
- Antigen for antibody production.
- Coating material for cell culture studies.
- Collagen microfibrils will activate platelets causing aggregation.
Note: May not be suitable for 3-D gel formation.
Related Products
ebiomall.com
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有几个疑问
1：荧光标记到细胞是标记到细胞表面还是细胞质内？
2：荧光应该随着细胞的分化和增殖逐渐消失？是不是分化增殖越快，荧光消失速度越快？
3：有哪些容易操作，成本便宜的荧光物质？
谢谢各位战友
1，逆转录病毒将GFP基因导入干细胞，这种办法在导入基因时好像比较麻烦，但是检测好像比较简单。
2，将雄性干细胞植入雌性动物，追踪Y染色体，即SRY基因，但是看文献上说SRY追踪的FISH技术复杂，费用高，但是还没有看到具体的技术步骤，也不知道具体要多少费用。
请多指教
