Hito Alcian Blue - PAS OptimStain™ Kit is formulated for the staining of neutral and acetic mucosubstances (Hyaluronic acid / sialomucins, Polysaccharides and neutral mucosubstances containing hexoses and deoxyhexoses with vicinal glycol groups). This Kit can be used with paraffin-embedded tissue sections (Frozen tissue section can be used, but not recommended).Hito Alcian Blue - PAS OptimStain™ Kit is designed in a ready-to-use format and offers high quality, rapid and reproducible staining.Hito Alcian Blue - PAS OptimStain™ Kit has been tested extensively on the tissues from several species of animals and it is a simple solution for your research.
| Kit Contents | |
| Solution-1 | 100 ml |
| Solution-2 (Alcian Blue Solution pH-2.5) | 100 ml |
| Solution-3 | 100 ml |
| Solution-4 | 100 ml |
| Solution-5 | 100 ml |
| Staining Jars | 5 |
| User Manual and MSDS | 1 |
Before using Hito Alcian Blue - PAS OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit): Microtome and light microscopeEthanol, Xylene, double distilled or deionized waterSlides and coverslipsStaining jars for slides washResinous mounting medium Hito Alcian Blue - PAS OptimStain™ Kit Manual and MSDS | |
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有几个疑问
1:荧光标记到细胞是标记到细胞表面还是细胞质内?
2:荧光应该随着细胞的分化和增殖逐渐消失?是不是分化增殖越快,荧光消失速度越快?
3:有哪些容易操作,成本便宜的荧光物质?
谢谢各位战友
1,逆转录病毒将GFP基因导入干细胞,这种办法在导入基因时好像比较麻烦,但是检测好像比较简单。
2,将雄性干细胞植入雌性动物,追踪Y染色体,即SRY基因,但是看文献上说SRY追踪的FISH技术复杂,费用高,但是还没有看到具体的技术步骤,也不知道具体要多少费用。
请多指教

