
Hito Alcian Blue - PAS OptimStain™ Kit is formulated for the staining of neutral and acetic mucosubstances (Hyaluronic acid / sialomucins, Polysaccharides and neutral mucosubstances containing hexoses and deoxyhexoses with vicinal glycol groups). This Kit can be used with paraffin-embedded tissue sections (Frozen tissue section can be used, but not recommended).Hito Alcian Blue - PAS OptimStain™ Kit is designed in a ready-to-use format and offers high quality, rapid and reproducible staining.Hito Alcian Blue - PAS OptimStain™ Kit has been tested extensively on the tissues from several species of animals and it is a simple solution for your research.
Kit Contents | |
Solution-1 | 100 ml |
Solution-2 (Alcian Blue Solution pH-2.5) | 100 ml |
Solution-3 | 100 ml |
Solution-4 | 100 ml |
Solution-5 | 100 ml |
Staining Jars | 5 |
User Manual and MSDS | 1 |
Before using Hito Alcian Blue - PAS OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit): Microtome and light microscopeEthanol, Xylene, double distilled or deionized waterSlides and coverslipsStaining jars for slides washResinous mounting medium ![]() |
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Thy-1、c-kit散在分布于HB组织中,主要集中在汇管区,而正常肝脏组织中不表达;CD34及SCF在HB中的表达明显高于正常肝脏组织(P0.05),其中CD34主要在血管内皮系统中分布,SCF主要表达在汇管区;CD56表达于成簇的神经纤维组织中,在HB及正常肝脏组织中表达差异无统计学意义(P0.05)。结论不同干细胞相关表面标志物分布于HB组织中,并且集中表达在特定区域。表达Thy-1/c-kit阳性细胞可能对HB的发生起一定作用。
1,逆转录病毒将GFP基因导入干细胞,这种办法在导入基因时好像比较麻烦,但是检测好像比较简单。
2,将雄性干细胞植入雌性动物,追踪Y染色体,即SRY基因,但是看文献上说SRY追踪的FISH技术复杂,费用高,但是还没有看到具体的技术步骤,也不知道具体要多少费用。
请多指教
Thy-1、c-kit散在分布于HB组织中,主要集中在汇管区,而正常肝脏组织中不表达;CD34及SCF在HB中的表达明显高于正常肝脏组织(P0.05),其中CD34主要在血管内皮系统中分布,SCF主要表达在汇管区;CD56表达于成簇的神经纤维组织中,在HB及正常肝脏组织中表达差异无统计学意义(P0.05)。结论不同干细胞相关表面标志物分布于HB组织中,并且集中表达在特定区域。表达Thy-1/c-kit阳性细胞可能对HB的发生起一定作用。
有几个疑问
1:荧光标记到细胞是标记到细胞表面还是细胞质内?
2:荧光应该随着细胞的分化和增殖逐渐消失?是不是分化增殖越快,荧光消失速度越快?
3:有哪些容易操作,成本便宜的荧光物质?
谢谢各位战友

