| BayCysLT2CysLT2 receptor antagonist |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
Nature.2018 Nov;563(7731):407-411.Nature.2018 Jun 13.Nature.2018 Jun 27.Nature.2018 Mar 29;555(7698):673-677.Nature.2017 Sep 7;549(7670):96-100.Nature.2016 Apr 21;532(7599):398-401.
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- Purity ≥95.00%
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| Cas No. | 712313-33-2 | SDF | Download SDF |
| Synonyms | CAY10633 | ||
| Chemical Name | 3-[[(3-carboxycyclohexyl)amino]carbonyl]-4-[3-[4-(4-phenoxybutoxy)phenyl]propoxy]-benzoic acid | ||
| Canonical SMILES | O=C(NC1CCCC(C(O)=O)C1)C2=CC(C(O)=O)=CC=C2OCCCC(C=C3)=CC=C3OCCCCOC4=CC=CC=C4 | ||
| Formula | C34H39NO8 | M.Wt | 589.7 |
| Solubility | ≤20mg/ml in DMSO;20mg/ml in dimethyl formamide | Storage | Store at -20°C |
| Physical Appearance | A crystalline solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
BayCysLT2, an isophthalic acid derivative, is a selective and potent CysLT2 receptor antagonist [1].
Cysteinyl leukotrienes (CysLTs) belong to a family of G protein-coupled receptors (GPCRs). The cysteinyl leukotrienes (CysLTs) are inflammatory mediators associated with neuronal injury after brain ischemia through the activation of their receptors, CysLT1R and CysLT2R [2].
In vitro: BayCysLT2 inhibited radioligand binding of LTD4 to CysLT2 and CysLT1 receptor cell lines with IC50 values of 35 and >10,000 nM, respectively [1]. BayCysLT2 reversed LTC4-stimulated perfusion pressure increase and contractility decrease in isolated Langendorff-perfused guinea pig hearts in a concentration-dependent manner [1]. BayCysLT2 protected astrocytes from ischemic injury [3].
In vivo: BayCysLT2 attenuated myocardial infarction damage but also inhibited LTD4-induced Evans blue dye leakage in the mouse ear vasculature [4].
References:[1] M. Harter, J. Erguden, F. Wunder, et al. Isophtalic acid derivatives. 10/537,623, 1-104 (2006).[2] Takasaki J, Kamohara M, Matsumoto M, et al. The molecular characterization and tissue distribution of the human cysteinyl leukotriene CysLT 2 receptor[J]. Biochemical and biophysical research communications, 2000, 274(2): 316-322.[3] X. J. Huang, W.P. Zhang, C.T. Li, W.Z. Shi, S.H. Fang, Y.B. Lu, Z. Chen, E.Q. Wei. Activation of CysLT receptors induces astrocyte proliferation and death after oxygen–glucose deprivation. Glia, 56 (2008), pp. 27–37[4] N. C. Ni, D. Yan, L.L. Ballantyne, A. Barajas-Espinosa St, T. Amand, D.A. Pratt, C.D. Funk. A selective cysteinyl leukotriene receptor 2 antagonist blocks myocardial ischemia/reperfusion injury and vascular permeability in mice. J. Pharmacol. Exp. Ther., 339 (2011), pp. 768–778
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1、 缓冲溶液对反应物的测定没有干扰
2、缓冲组分的浓度为1:1
3、有足够的缓冲容量
4、缓冲溶液的PH应在所需范围内
5、组成缓冲溶液的弱碱PKB和弱酸PKA应接近或等于所需的POH值或PH值（PH+POH=14）
配制
只要知道缓冲对的PH值，和要配制的缓冲液的pH值（及要求的缓冲液总浓度），就能按公式计算[盐]和[酸]的量。这个算法涉及对数换算，较麻烦，前人为减少后人的计算麻烦，已为我们总结出pH值与缓冲液对离子用量的关系并列出了表格。只要我们知道要配制的缓冲液的pH，经查表便可计算出所用缓冲剂的比例和用量。例如配制500nmpH5.8浓度为0.1M磷酸缓冲液。
经查表知pH5.8浓度为0.2M Na2HPO48.0毫升，而0.2M Na2HPO492.0毫升。依此可推论出配制100ml0.1M的磷酸缓冲液需要0.1M Na2HPO48.0毫升，而0.1M Na2HPO4需要92.0毫升。
计算好后，按计算结果准确称好固态化学成分，放于烧杯中，加少量蒸馏水溶解，转移入50ml容量瓶，加蒸馏水至刻度，摇匀，就能得到所需的缓冲液。
各种缓冲溶液的配制，均按表格按比例混合，某些试剂，必须标定配成准确浓度才能进行，如醋酸、氢氧化钠等。另外，所有缓冲溶剂的配制计量都能从以上的算式准确获得。
TBST中含有Tris-Hcl，NaCl，Tween20这三种物质，是做WESTERNBLOT中常用的一种缓冲液。
TBST缓冲液的配制
1000ml×TBST的配置
先称量NaCl40g，倒入烧杯中，加DDW蒸馏水400ml,再称量NaCl47.6g，倒入刚才的那个烧杯中（PS：由于NaCl的量太多，一次称量不方便，所以分两次称量，且易于溶解）。往烧杯中加入Tris—HCl缓冲液100ml，最后加（吐温20）5ml,转入1000ml容量瓶中，在定容，转移即可。
TBST缓冲液的应用：
1.主要用于免疫组化和原位杂交，酶联免疫等实验中，清洗免疫印。
2.迹膜；
注意事项：
1.TBST缓冲液，PH7.2-7.5；
2.颜色为无色透明液体；
3.为了您的安全和健康，请穿实验服并戴防护手套操作；
