- Melatonin
- Tolbutamide
| RamelteonAgonist of melatonin receptor(M1-M2),highly selective |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
Nature.2018 Nov;563(7731):407-411.Nature.2018 Jun 13.Nature.2018 Jun 27.Nature.2018 Mar 29;555(7698):673-677.Nature.2017 Sep 7;549(7670):96-100.Nature.2016 Apr 21;532(7599):398-401.
Science.2016 Aug 5;353(6299)594-8Nat Nanotechnol.2017 Dec;12(12):1190-1198.Nature Biotechnology.2017 Jun;35(6):569-576Nat Med.2018 Sep 17.Cell.2018 Dec 21. pii: S0092-8674(18)31561-7.Cell.Available online 25 October 2018.Cell.2018 Sep 27. pii: S0092-8674(18)31183-8.Cell.2018 Jun 28;174(1):172-186.e21.Cell.2018 Feb 22;172(5):1007-1021.e17.Cell.2017 Nov 30;171(6):1284-1300.e21.Cell.2017 Aug 17. pii: S0092-8674(17)30869-3.Cell.2017 Jul 13;170(2):312-323Nat Med.2018 Jan 29.Nat Med.2017 Nov;23(11):1342-1351.Cell.2017 Apr 6;169(2):286-300.Cell.2015 Aug 27;162(5):987-1002.Cell.2015 Feb 12;160(4):729-44.Nature Medicine.2017 Apr;23(4):493-500.Cancer Cell.2018 May 14;33(5):905-921.e5.Cancer Cell.2018 Apr 9;33(4):752-769.e8.Cancer Cell.2018 Mar 12;33(3):401-416.e8.Cancer Cell.2017 Aug 14;32(2):253-267.e5.Nat Methods.2018 Jul;15(7):523-526.Cell Stem Cell.2018 May 3;22(5):769-778.e4.Cell Stem Cell.2017 Nov 20. pii: S1934-5909(17)30375-2.Quality Control & MSDS
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- Purity = 99.89%
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- HPLC(Retest)
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- MSDS (Material Safety Data Sheet)
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Chemical structure
| Description | Ramelteon is a selective agonist of MT1/MT2 with Ki values of 0.014 nM and 0.045 nM, respectively. | |||||
| Targets | MT1 | MT2 | ||||
| IC50 | 0.014 nM (Ki) | 0.045 nM (Ki) | ||||
| Kinase experiment [1]: | |
Binding assays | Cells stably expressing MT1 or MT2 receptors (CHO-hMelR7) were selected and cultured in Eagle’s Minimum Essential Medium-α (MEM-α) supplemented with 10% dialyzed fetal bovine serum (dFBS) under a 5% CO2/95% air atmosphere. Cells were harvested at confluence in Ca2+-Mg2+ free Hanks’ balanced salt solution containing 5 mM EDTA and collected by centrifugation. Cells were homogenized in ice-cold 50 mM Tris-HCl buffer (pH 7.7 at 25℃), washed twice, pelleted, and stored at -30℃ until the binding assays were conducted. Ramelteon and 40 pM 2-[125I]melatonin were mixed with the thawed homogenate in a total volume of 1 mL and incubated at 25℃ for 150 min. The reaction was terminated by addition of 3 mL of icecold buffer followed by vacuum filtration on a Whatman GF/B. The filter was washed twice and radioactivity was counted by a γ-counter. Nonspecific binding was defined as the binding in the presence of 10 mM melatonin. |
| Cell experiment [2]: | |
Cell lines | Pancreatic INS-1 β-cells |
Preparation method | The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reaction Conditions | 2-14 h |
Applications | Ramelteon is a potent and selective agonist of MT1/MT2 melatonin receptors. Ramelteon inhibits forskolin-stimulated cAMP production in the CHO cells that express the human MT1 or MT2 receptors [2]. Ramelteon is capable of increasing brain-derived neurotrophic factor (BDNF) protein in neurons expressing either MT1 or MT2 receptor type in mouse cerebellar granule cells [3]. |
| Animal experiment [4]: | |
Animal models | Freely moving cats |
Dosage form | 0.001, 0.01, and 0.1 mg/kg; administered orally. |
Preparation method | Suspended in a 0.5% (weight per volume) methylcellulose solution. |
Applications | In cats, ramelteon has a sleep-promoting action and does not appear to cause learning, memory, or motor function impairment, or to have rewarding properties [4]. In a clinical study, ramelteon decreases latency to persistent sleep and increases total sleep time and sleep efficiency in subjects with primary chronic insomnia [1]. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1]. Kato K, Hirai K, Nishiyama K, et al. Neurochemical properties of ramelteon (TAK-375), a selective MT1/MT2 receptor agonist. Neuropharmacology, 2005, 48(2): 301-310. [2]. Nishiyama K, Hirai K. The melatonin agonist ramelteon induces duration-dependent clock gene expression through cAMP signaling in pancreatic INS-1 β-cells. PLoS One, 2014, 9(7): e102073. [3]. Imbesi M, Uz T, Dzitoyeva S, et al. Stimulatory effects of a melatonin receptor agonist, ramelteon, on BDNF in mouse cerebellar granule cells. Neurosci Lett, 2008, 439(1): 34-36. [4]. Miyamoto M, Nishikawa H, Doken Y, et al. The sleep-promoting action of ramelteon (TAK-375) in freely moving cats. Sleep, 2004, 27(7): 1319-1325. | |
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| Cas No. | 196597-26-9 | SDF | Download SDF |
| Synonyms | N/A | ||
| Chemical Name | N-[2-[(8S)-2,6,7,8-tetrahydro-1H-cyclopenta[e][1]benzofuran-8-yl]ethyl]propanamide | ||
| Canonical SMILES | CCC(=O)NCCC1CCC2=C1C3=C(C=C2)OCC3 | ||
| Formula | C16H21NO2 | M.Wt | 259.34 |
| Solubility | ≥12.95mg/mL in DMSO | Storage | Store at -20°C |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Ramelteon, a chronohypnotic, is an orally active and highly selective melatonin receptor (MT1, MT2 and MT3) agonist that preferentially binds to MT1 and MT2 receptors leading to potent inhibition of cAMP production in human cells expressing MT1 or MT2 receptors with the half maximal inhibition concentration IC50 values of 21.2 pM/L and 53.4 pM/L respectively [1].
The s-configuration and ether group in the chemical structure of ramelteon confer its high affinity towards MT1 and MT2 receptors with the dissociation constant Ki values of 14 pM and 112 pM respectively in comparison with that of 2650 nM for MT3 receptors [1].
Ramelteon has been found to potently promote sleep without causing any significant adverse effects leading to its application for the treatment of insomnia [1].
References:[1] Miyamoto M. Pharmacology of ramelteon, a selective MT1/MT2 receptor agonist: a novel therapeutic drug for sleep disorders. CNS Neurosci Ther. 2009 Winter;15(1):32-51. doi: 10.1111/j.1755-5949.2008.00066.x.
ebiomall.com
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对不起没有没有财富值了
原理：利用温度传感器测量，传入温控器进行PID测算，输出0-10V或0-20ma的模拟信号，再通过模拟信号控制阀门控制器控制阀门的打开与关闭，来达到调节流量或压力的目的。
如果是要控制恒流量或恒压力，那么温度控制器是不行的啦。
请教
安捷伦1260高效液相，先做五针对照品一的峰面积RSD为0.4%，然后进第二个对照品时第一针峰面积2238，第二针的为2330，此机器刚做完校验而且前一天的实验也一切正常，灯也是刚换不久。样品瓶中也不存在有气泡或样品溶液装得太满的问题。而且样品盘有温控，亦没有发现漏液。自己实在是想不到哪有问题，难不成真是机器偶尔秀逗了一下？有没有大神给点意见………………
我们现在要插到瓶子里，做个验证，初步计划：
1、空载验证探头和温控探头都放在腔室内，确定腔室的冷点。
2、半载、满载的时候温控探头插在灌水的瓶子里，放在冷点处，验证的探头均匀放在腔室内。
3、穿透的时候所有探头都插到瓶子里。控温探头放到冷点、验证探头均匀分布。
大家帮忙看下这样有没有什么风险，有没有更好的主意（本人总是觉得有些问题）
2、其工作原理是通过温度传感器对环境温度自动进行采样、即时监控，当环境温度高于控制设定值时控制电路启动，可以设置控制回差。如温度还在升，当升到设定的超限报警温度点时，启动超限报警功能。当被控制的温度不能得到有效的控制时，为了防止设备的毁坏还可以通过跳闸的功能来停止设备继续运行。主要应用于电力部门使用的各种高低压开关柜、干式变压器、箱式变电站及其他相关的温度使用领域。
这里我想像大家介绍一种新的高效细胞活性和细胞增殖测定剂——alamarBlue。介绍之前先说最重要的一点，使用alamarBlue价格比较昂贵，如果你的银子不多，呵呵，还是乖乖告诉老板你的MTT结果吧。
细胞增殖检测主要有三种方法：第一、增殖期抗体的测定（例如Ki-67和CydlinE）。第二、增殖期DNA合成标记（3H,BrdU等）。相信这两种方法少有人做，反正以前我都没听过（见笑了）。还有第三就是增殖细胞对外界环境物质的降解测定（包括大家常做的MTT和XTT、MTS，还有今天要介绍的AlamarBlue）。
AlamarBlue是一种新型的细胞活性和细胞增殖的指示剂，是一种可溶、稳定、无毒的靛蓝染料，同时适用于贴壁和悬浮细胞。它对细胞抗原的表达和杂交瘤细胞的抗体分泌功能无影响，而且非常有意义的一点就是，经过AlamarBlue测定的细胞可以继续培养或进行其他的功能测定。AlamarBlue可以用普通分光光度计、荧光光度计和酶标仪进行测定，非常方便，其测定结果同样有线性趋势，而且线性范围更广，更灵敏和稳定。
主要应用于：
a.优化细胞培养条件
b.对细胞生长因子或细胞因子进行活性定量
c.有助于开发抗生素或抗肿瘤的新药
d.了解毒性物质或污染物对细胞生长的影响
e.评估细胞介导的细胞毒作用
f.定量检测凋亡发生
g.适用于真核细胞、真菌和细菌的活性和增殖测定。
附件是AlamarBlue的产品详细说明，实验操作手册。感兴趣的战友可以看一看。还有一篇文章是比较MTT和AlamarBlue的，不过很遗憾，我没能查到全文，如果哪位高手能查出来，真心希望能够贴出来与大家分享。
Hamid,R.,Rotshteyn,Y.,RabADI,L.etal.ComparisonofalamarblueandMTTassaysforhighthrough-putscreening.ToxicologyinVitro.200418(5);703-710.
alamarbluebooklet.pdf(153.16k)
因为做免疫组化前固定的标本一般不超过48－72小时，所以较急！在线等！
请不要说用某某进口仪器，希望有些土办法。
在欧洲绝大多情况是温控器是壁挂炉必配件，两者一配一的同时交付用户的，而且配备的温控器大多是智能型温控器。而在国内，已安装在运行和正在安装调试准备投入使用的壁挂炉近95%的没有先行配备任何形式的简易或智能型的温控器。而房间采暖系统中配备温控器尤其是智能温控器，是节能采暖综合体系中一个极为突出的最重要的环节。
方便——每天自动定时提前或延后开关调节壁挂炉，免去人工操作，对上班族家庭最有必要；
舒适——每天早午晚夜各时段室温自动高低调整，免去早晨起床和下班回家后等待房间升温而挨冻的尴尬；
省气——改落后粗放的水温控制为先进准确的室温控制，加上分时段定室温按需运行，不用敞开的昼夜烧气采暖；
放心——室温过低时强制启动壁挂炉，仅需极少的燃气，便可安全的进行居室防冻保护。
温控器一般安在人多的客厅的，和办公室的空调开关一样，它是有线连接。在装修时要留一条由壁挂炉底部到客厅的暗线管，内留两根多股的胶线。
有机械式的和电子式的，
机械式的采用两层热膨胀系数不同金属压在一起，温度改变时，他的弯曲度会发生改变，当弯曲到某个程度时，接通（或断开）回路，使得制冷（或加热）设备工作。
电子式的通过热电偶、铂电阻等温度传感装置，把温度信号变换成电信号，通过单片机、PLC等电路控制继电器使得加热（或制冷）设备工作（或停止）。
还有水银温度计型的,温度到就会有触点和水银接通
