Recombinant Human HA-SUMO1 Vinyl Sulfone Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UL-703
| Formulation | Supplied as a solution in HEPES, NaCl, Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: SUMO1
Human Small Ubiquitin-like Modifier 1 (SUMO1), also known as Sentrin, UBL1, and SMT3C, is synthesized as a 101 amino acid (aa) propeptide with a predicted molecular weight of 11.5 kDa. Human SUMO1 is the most unique of the four identified SUMO proteins and shares only 44%, 47%, and 41% aa sequence identity with SUMO2, SUMO3, and SUMO4, respectively. In contrast, human SUMO1 shares 100% aa sequence identity with the mouse ortholog. SUMOs are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed SUMOylation (1-3). All SUMO proteins share a conserved Ubiquitin domain and a C-terminal diglycine cleavage/attachment site. Following cleavage of a four aa C-terminal prosegment, the C-terminal glycine residue of SUMO1 is enzymatically attached to a lysine residue on a target protein. In humans, SUMO1 is conjugated to a variety of molecules in the presence of the SAE1/UBA2 SUMO-activating (E1) enzyme and the UBE2I/Ubc9 SUMO-conjugating (E2) enzyme (4,5). In yeast, the SUMO-activating (E1) enzyme is Aos1/Uba2p (6). SUMOylation can occur without the requirement of a specific SUMO ligase (E3), where SUMO1 is transferred directly from UBE2I/Ubc9 to specific substrates. In Alzheimer"s disease models SUMO1 has been shown to influence the generation of Amyloid-beta peptide by promoting the accumulation of BACE-1 (7). Covalent modification of Phosphatase and Tensin Homolog Deleted on Chromosome (PTEN) by SUMO1 is thought to regulate tumorigenesis by retaining PTEN at the plasma membrane, an effect that suppresses PI 3-Kinase/Akt-dependent tumor growth (8).
This N-terminal HA-tagged SUMO is a potent, irreversible and specific inhibitor of SUMO-specific proteases (SENPs).This protein inhibits the hydrolysis of poly-SUMO chains on substrate proteins in vitro and thus enhances poly-SUMO chain accumulation.The HA peptide sequence (YPYDVPDYA) is derived from the influenza Hemagglutinin protein.This epitope allows for the sensitive identification or purification of SENP activities since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.
- Desterro, J.M. et al. (1997) FEBS. Lett. 417:297.
- Bettermann, K. et al. (2012) Cancer Lett. 316:113.
- Praefcke, G.J. et al. (2012) Trends Biochem. Sci. 37:23.
- Okuma, T. et al. (1999) Biochem. Biophys. Res. Commun. 254:693.
- Tatham, M.H. et al. (2001) J. Biol. Chem. 276:35368.
- Johnson, E.S. et al. (1997) EMBO J. 16:5509.
- Yun, S.M. et al. (2012) Neurobiol Aging. [Epub ahead of print].
- Huang, J. et al. (2012) Nat. Commun. 3:911.
Citation for Recombinant Human HA-SUMO1 Vinyl Sulfone Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome StabilityAuthors: P Haahr, N Borgermann, X Guo, D Typas, D Achuthanku, S Hoffmann, R Shearer, TK Sixma, N MailandMol. Cell, 2018;0(0):.Species: HumanSample Types: Cell LysatesApplications: Immunoprecipitation
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对不起没有没有财富值了
原理：利用温度传感器测量，传入温控器进行PID测算，输出0-10V或0-20ma的模拟信号，再通过模拟信号控制阀门控制器控制阀门的打开与关闭，来达到调节流量或压力的目的。
如果是要控制恒流量或恒压力，那么温度控制器是不行的啦。
请教
安捷伦1260高效液相，先做五针对照品一的峰面积RSD为0.4%，然后进第二个对照品时第一针峰面积2238，第二针的为2330，此机器刚做完校验而且前一天的实验也一切正常，灯也是刚换不久。样品瓶中也不存在有气泡或样品溶液装得太满的问题。而且样品盘有温控，亦没有发现漏液。自己实在是想不到哪有问题，难不成真是机器偶尔秀逗了一下？有没有大神给点意见………………
我们现在要插到瓶子里，做个验证，初步计划：
1、空载验证探头和温控探头都放在腔室内，确定腔室的冷点。
2、半载、满载的时候温控探头插在灌水的瓶子里，放在冷点处，验证的探头均匀放在腔室内。
3、穿透的时候所有探头都插到瓶子里。控温探头放到冷点、验证探头均匀分布。
大家帮忙看下这样有没有什么风险，有没有更好的主意（本人总是觉得有些问题）
2、其工作原理是通过温度传感器对环境温度自动进行采样、即时监控，当环境温度高于控制设定值时控制电路启动，可以设置控制回差。如温度还在升，当升到设定的超限报警温度点时，启动超限报警功能。当被控制的温度不能得到有效的控制时，为了防止设备的毁坏还可以通过跳闸的功能来停止设备继续运行。主要应用于电力部门使用的各种高低压开关柜、干式变压器、箱式变电站及其他相关的温度使用领域。
这里我想像大家介绍一种新的高效细胞活性和细胞增殖测定剂——alamarBlue。介绍之前先说最重要的一点，使用alamarBlue价格比较昂贵，如果你的银子不多，呵呵，还是乖乖告诉老板你的MTT结果吧。
细胞增殖检测主要有三种方法：第一、增殖期抗体的测定（例如Ki-67和CydlinE）。第二、增殖期DNA合成标记（3H,BrdU等）。相信这两种方法少有人做，反正以前我都没听过（见笑了）。还有第三就是增殖细胞对外界环境物质的降解测定（包括大家常做的MTT和XTT、MTS，还有今天要介绍的AlamarBlue）。
AlamarBlue是一种新型的细胞活性和细胞增殖的指示剂，是一种可溶、稳定、无毒的靛蓝染料，同时适用于贴壁和悬浮细胞。它对细胞抗原的表达和杂交瘤细胞的抗体分泌功能无影响，而且非常有意义的一点就是，经过AlamarBlue测定的细胞可以继续培养或进行其他的功能测定。AlamarBlue可以用普通分光光度计、荧光光度计和酶标仪进行测定，非常方便，其测定结果同样有线性趋势，而且线性范围更广，更灵敏和稳定。
主要应用于：
a.优化细胞培养条件
b.对细胞生长因子或细胞因子进行活性定量
c.有助于开发抗生素或抗肿瘤的新药
d.了解毒性物质或污染物对细胞生长的影响
e.评估细胞介导的细胞毒作用
f.定量检测凋亡发生
g.适用于真核细胞、真菌和细菌的活性和增殖测定。
附件是AlamarBlue的产品详细说明，实验操作手册。感兴趣的战友可以看一看。还有一篇文章是比较MTT和AlamarBlue的，不过很遗憾，我没能查到全文，如果哪位高手能查出来，真心希望能够贴出来与大家分享。
Hamid,R.,Rotshteyn,Y.,RabADI,L.etal.ComparisonofalamarblueandMTTassaysforhighthrough-putscreening.ToxicologyinVitro.200418(5);703-710.
alamarbluebooklet.pdf(153.16k)
因为做免疫组化前固定的标本一般不超过48－72小时，所以较急！在线等！
请不要说用某某进口仪器，希望有些土办法。
在欧洲绝大多情况是温控器是壁挂炉必配件，两者一配一的同时交付用户的，而且配备的温控器大多是智能型温控器。而在国内，已安装在运行和正在安装调试准备投入使用的壁挂炉近95%的没有先行配备任何形式的简易或智能型的温控器。而房间采暖系统中配备温控器尤其是智能温控器，是节能采暖综合体系中一个极为突出的最重要的环节。
方便——每天自动定时提前或延后开关调节壁挂炉，免去人工操作，对上班族家庭最有必要；
舒适——每天早午晚夜各时段室温自动高低调整，免去早晨起床和下班回家后等待房间升温而挨冻的尴尬；
省气——改落后粗放的水温控制为先进准确的室温控制，加上分时段定室温按需运行，不用敞开的昼夜烧气采暖；
放心——室温过低时强制启动壁挂炉，仅需极少的燃气，便可安全的进行居室防冻保护。
温控器一般安在人多的客厅的，和办公室的空调开关一样，它是有线连接。在装修时要留一条由壁挂炉底部到客厅的暗线管，内留两根多股的胶线。
有机械式的和电子式的，
机械式的采用两层热膨胀系数不同金属压在一起，温度改变时，他的弯曲度会发生改变，当弯曲到某个程度时，接通（或断开）回路，使得制冷（或加热）设备工作。
电子式的通过热电偶、铂电阻等温度传感装置，把温度信号变换成电信号，通过单片机、PLC等电路控制继电器使得加热（或制冷）设备工作（或停止）。
还有水银温度计型的,温度到就会有触点和水银接通
