CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF)
ATF1; ATF2; HFB1-GDNF; Astrocyte-Derived Trophic Factor; Glial Derived Neurotrophic Factor
- Product No.SCA043Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range13.7-10,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.2pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cerebrospinal fluid, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 588 For more details, please contact local distributors!US$ 840 For more details, please contact local distributors!US$ 3780 For more details, please contact local distributors!US$ 7140 For more details, please contact local distributors!US$ 58800 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF)
This assay has high sensitivity and excellent specificity for detection of Glial Cell Line Derived Neurotrophic Factor (GDNF).No significant cross-reactivity or interference between Glial Cell Line Derived Neurotrophic Factor (GDNF) and analogues was observed.
Recovery of the CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF)
Matrices listed below were spiked with certain level of recombinant Glial Cell Line Derived Neurotrophic Factor (GDNF) and the recovery rates were calculated by comparing the measured value to the expected amount of Glial Cell Line Derived Neurotrophic Factor (GDNF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-101 | 96 |
| EDTA plasma(n=5) | 87-96 | 90 |
| heparin plasma(n=5) | 80-92 | 81 |
Precision of the CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor (GDNF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor (GDNF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glial Cell Line Derived Neurotrophic Factor (GDNF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 84-94% | 99-105% | 98-105% |
| EDTA plasma(n=5) | 99-105% | 81-98% | 78-91% | 86-95% |
| heparin plasma(n=5) | 89-97% | 92-99% | 80-101% | 83-103% |
Stability of the CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;8. Read RLU value immediately.
Test principle of the CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF)
The microplate provided in this kit has been pre-coated with an antibody specific to Glial Cell Line Derived Neurotrophic Factor (GDNF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Glial Cell Line Derived Neurotrophic Factor (GDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Glial Cell Line Derived Neurotrophic Factor (GDNF) level in the sample or standard.;
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ELISA / CLIA Experiment Service
INCREMENT SERVICES
Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIACLIA Kit Customized ServiceDisease Model Customized ServiceSerums Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental ServiceStreptavidin
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APA043Hu01 | Active Glial Cell Line Derived Neurotrophic Factor (GDNF) | Cell culture; Activity Assays. |
| RPA043Hu01 | Recombinant Glial Cell Line Derived Neurotrophic Factor (GDNF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA043Hu01 | Polyclonal Antibody to Glial Cell Line Derived Neurotrophic Factor (GDNF) | WB; IHC; ICC; IP. |
| LAA043Hu71 | Biotin-Linked Polyclonal Antibody to Glial Cell Line Derived Neurotrophic Factor (GDNF) | WB; IHC; ICC. |
| LAA043Hu81 | FITC-Linked Polyclonal Antibody to Glial Cell Line Derived Neurotrophic Factor (GDNF) | WB; IHC; ICC; IF. |
| MAA043Hu22 | Monoclonal Antibody to Glial Cell Line Derived Neurotrophic Factor (GDNF) | WB; IHC; ICC; IP. |
| SEA043Hu | ELISA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA043Hu | CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) | Chemiluminescent immunoassay for Antigen Detection. |
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（第1张图和第2张图是经旋转后方向恰好相反的两张图，上矢状窦等静脉也显示了）
女患，70岁，大专毕业，我院退休药师。7-8年前患脑梗（具体不详）。记忆力差5-6年，近记忆为著，几次做饭后忘记关火，将炉具的台板（玻璃的）烧裂。近5-6年来，病人每年住院彻底检查治疗1次（主要是使用活血化瘀及营养脑细胞药）。平素，病人双耳听力略差，睡眠欠佳、便秘、尿频，偶从卧位坐起时视蒙。既往有时血压略高，未降压治疗。本次为“通血管”再来住院。查体：血压：140/80mmHg,双耳听力略差，近记忆力差，但智力（MMSE：26分)正常，颅神经未见异常，四肢肌力、感觉未见异常，植物神经未查，双掌颌反射（+），双下肢病理反射均阴性。血常规：淋巴细胞比率略高，余均正常；尿常规、心电图、癌胚抗原均正常；凝血四项：除凝血酶时间略长外均正常；生化全项：除总胆固醇略高外均正常；超声心动图：老年瓣退行性改变，左室舒张功能减退，房膜瘤可能。彩超：双侧颈动脉粥样硬化形成。头MRA:右大脑中动脉及其分支未显示。
问题：1MRI怎么会这样逍遥？病人无肢体瘫，生活自理如常人。
2为明确头MRA:右大脑中动脉及其分支未显示的病因和程度，除做DSA外，还需做什么？
3如不做DSA，给他订类药、阿斯匹林及活血化瘀的中药可不可以？
教。
版主laocao留言:
患者的年龄，病史，症状，辅助检查传上来，便于进一步讨论，谢谢！
