QuantideX® NGS DNA Hotspot 21 Kit
The QuantideX® NGS DNA Hotspot 21 Kit (RUO) is a next generation sequencing (NGS) research tool that interrogates 46 hotspot regions (amplicons) within 21 genes that are commonly mutated in a number of solid and hematological malignancies. The kit detects over 1,500 known variants, including single nucleotide variants (SNVs), insertions/deletions (indels), and structural rearrangements. Leveraging our proprietary NGS-in-a-Box™ workflow and Sample-Aware™ bioinformatics quality control solutions, this kit provides a simple, robust, and reliable NGS assay for the routine investigation of these genomic variants.
Features & Benefits
The QuantideX NGS DNA Hotspot 21 Kit combines Asuragen’s unique NGS-in-a-Box™ solution with a streamlined testing workflow to enable unprecedented NGS workflow efficiency and high sensitivity at low input amounts from precious FFPE samples.
Reduced ComplexityAssay incorporates sample-to-data solutions in a unique NGS-in-a-Box™ configuration
- Detects >1,500 variants from commonly mutated genomic regions across multiple tumor types
- End-to-end kitted solution
- Fully integrated data analysis pipeline
Optimized WorkflowProvides operational efficiencies to reduce testing costs, hands-on and total turnaround time
- Reduced labor required for library preparation
- Improved turnaround time enables higher throughput
- Common workflow across portfolio streamlines training & implementation
Quality PerformanceHighly sensitive assay with integrated, Sample-Aware™ bioinformatics software and built-in quality checks to minimize erroneous results and sample failures
- Highly sensitive detection of DNA-based variants
- Low input (~20ng) of DNA from FFPE
- Sample-Aware™ bioinformatics analysis and sample quality control
Product Description
Relevant ContentThe QuantideX NGS DNA Hotspot 21 Kit detects over 1,500 genomic variants as reported in the Catalogue of Somatic Mutations in Cancer (COSMIC) Database within 21 genes, representing approximately 80% of known variants within these targets.
Table 1: Mutation coverage of QuantideX NGS DNA Hotspot 21 Kit
Table 2: Clinical relevance of covered mutations
1NCCN Clinical Practice Guidelines*Includes gastric, pancreatic, glicomas, sarcomas, and other tumor types
A Fully Integrated WorkflowA unique NGS-in-a-Box™ configuration offers a simplified and fully integrated NGS workflow with cGMP-manufactured reagents, components and controls ready to use.
Includes:
- An internal quality control kit to measure the absolute copy number of amplifiable DNA and reports PCR inhibition
- Gene-specific PCR primers and Master Mix reagents
- Dual-index barcodes for sample multiplexing
- Library purification and quantification reagents
- Integrated data analysis and reporting software (QuantideX NGS Reporter)
Rapid & EfficientAdopt and run NGS-based analysis with minimum investment of time and resources, regardless of NGS experience and infrastructure.
- Fully integrated workflow reduces complexity and ensures reliable reagent quality
- Push-button analysis & reporting makes bioinformatics easy and efficient, regardless of experience level
- Integrated kit reduces overall workflow time
Performance Data
Highly Sensitive and Accurate Detection of DNA Mutations
QuantideX® NGS Reporter
Push-button analytics & reporting suite – Set-up-and-go workflow designed for easy installation and implementation, right out of the box.
Operates on a standard desktop computer – Install locally on a desktop computer using Windows® operating system. No prior bioinformatics experience or large server environments required.
Comprehensive– Full bioinformatics and reporting of variants (SNVs, Indels), and standard QC metrics are automatically calculated.
Integrates Sample-Aware™ – Bioinformatics with integrated functional template copy number analysis dramatically reduces false-call rates.
Resources
Videos
Next-Generation Sequencing Within Your Reach: Implementation of an Actionable Mutation Panel for Molecular Oncology Testing
Posters
Analytical Validation Of The QuantideX® NGS DNA Hotspot 21 Kit, A Diagnostic NGS System for the Detection of Actionable Mutations in FFPE TumorsView full poster
A Machine-Learning Framework for Accurate Classification and Quantification of Oncogenic Variants Using the QuantideX® NGS DNA Hotspot 21 KitView full poster
A Simple and Versatile Next-Generation Sequencing Technology for Co-Detection of RNA Structural Variants and DNA Mutations in Lung CancerView full poster
Ordering
| Product Name | Number of Reactions | Catalog Number |
|---|---|---|
| QuantideX NGS DNA Hotspot 21 Kit* | 48 | 46108 |
T 1.877.777.1874 | 512.681.5200 F 512.681.5202 E orders@asuragen.com
*For Research Use Only. Not for use in Diagnostic procedures.
QuantideX® NGS DNA Hotspot 21 Kit
The QuantideX® NGS DNA Hotspot 21 Kit is an in vitro diagnostic, next-generation sequencing (NGS) panel for the detection of clinically relevant variants across a multitude of tumor types, including non-small cell lung cancer, colorectal cancer, and melanoma. The kit screens for over 1,500 known genomic variants, including single nucleotide variants (SNVs), insertions/deletions (indels), and structural rearrangements, many of which are treatable with novel therapies, inform on patient management, or are the subject of further clinical evaluation. Leveraging our proprietary NGS-in-a-Box™ workflow and Sample-Aware™ bioinformatics quality control solutions, the panel provides a robust and reliable NGS solution for the identification of clinically relevant targets you and your clinicians can trust.
Features & Benefits
The QuantideX NGS DNA Hotspot 21 Kit combines Asuragen’s unique NGS-in-a-Box™ solution with a streamlined testing workflow to enable unprecedented NGS workflow efficiency and high sensitivity at low input amounts from precious FFPE samples.
Reduced ComplexityAssay incorporates sample-to-data solutions in a unique NGS-in-a-Box™ configuration
- Detects >1,500 variants from commonly mutated genomic regions across multiple tumor types
- End-to-end kitted solution
- Fully integrated data analysis pipeline
Optimized WorkflowProvides operational efficiencies to reduce testing costs, hands-on and total turnaround time
- Reduced labor required for library preparation
- Improved turnaround time enables higher throughput
- Common workflow across portfolio streamlines training & implementation
Quality PerformanceHighly sensitive assay with integrated, Sample-Aware™ bioinformatics software and built-in quality checks to minimize erroneous results and sample failures
- Highly sensitive detection of DNA-based variants
- Low input (~20ng) of DNA from FFPE
- Sample-Aware™ bioinformatics analysis and sample quality control
Product Description
Relevant ContentThe QuantideX NGS DNA Hotspot 21 Kit detects over 1,500 genomic variants as reported in the Catalogue of Somatic Mutations in Cancer (COSMIC) Database within 21 genes, representing approximately 80% of known variants within these targets. Several of these variants are associated with approved therapies, while others are currently in trials to clarify their clinical significance.
Table 1: Mutation coverage of QuantideX NGS DNA Hotspot 21 Kit
Table 2: Clinical relevance of covered mutations
1NCCN Clinical Practice Guidelines*Includes gastric, pancreatic, glicomas, sarcomas, and other tumor types
A Fully Integrated WorkflowA unique NGS-in-a-Box™ configuration offers a simplified and fully integrated NGS workflow with cGMP-manufactured reagents, components and controls ready to use.
Includes:
- An internal quality control kit to measure the absolute copy number of amplifiable DNA and reports PCR inhibition
- Gene-specific PCR primers and Master Mix reagents
- Dual-index barcodes for sample multiplexing
- Library purification and quantification reagents
- Integrated data analysis and reporting software (QuantideX NGS Reporter)
Rapid & EfficientAdopt and run NGS-based analysis with minimum investment of time and resources, regardless of NGS experience and infrastructure.
- Fully integrated workflow reduces complexity and ensures reliable reagent quality
- Push-button analysis & reporting makes bioinformatics easy and efficient, regardless of experience level
- Integrated kit reduces overall workflow time
Performance Data
Highly Sensitive and Accurate Detection of DNA Mutations
QuantideX® NGS Reporter
Push-button analytics & reporting suite – Set-up-and-go workflow designed for easy installation and implementation, right out of the box.
Operates on a standard desktop computer – Install locally on a desktop computer using Windows® operating system. No prior bioinformatics experience or large server environments required.
Comprehensive – Full bioinformatics and reporting of variants (SNVs, Indels) and standard QC metrics are automatically calculated.
Integrates Sample-Aware™ – bioinformatics with integrated functional template copy number analysis dramatically reduces false-call rates.
Resources
Videos
Next-Generation Sequencing Within Your Reach: Implementation of an Actionable Mutation Panel for Molecular Oncology Testing
Posters
Analytical Validation Of The QuantideX® NGS DNA Hotspot 21 Kit, A Diagnostic NGS System for the Detection of Actionable Mutations in FFPE TumorsView full poster
A Machine-Learning Framework for Accurate Classification and Quantification of Oncogenic Variants Using the QuantideX® NGS DNA Hotspot 21 KitView full poster
A Simple and Versatile Next-Generation Sequencing Technology for Co-Detection of RNA Structural Variants and DNA Mutations in Lung CancerView full poster
Ordering
| Product Name | Number of Reactions | Catalog Number |
|---|---|---|
| QuantideX NGS DNA Hotspot 21 Kit* | 48 | 76044 |
T 1.877.777.1874 | 512.681.5200 F 512.681.5202 E orders@asuragen.com
*For Research Use Only. Not for use in Diagnostic procedures.
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移液器又称移液枪，是一种用于定量转移液体的器具，被广泛用于生物、化学等领域。
特点：
移液杆可360旋转,不用工具可卸下121℃高温灭菌。
符合人体工程学的外型设计。
符合ISO9000和GLP规定，用户可独立校准。数字可调式移液器校准不需要工具
固定式移液器校准只需要需要小型工具。
有较高的精度和误差系数。
吸头接嘴有独特的V形圈，可以适合不同品牌的吸头。
每把多道移液器赠送每支移液器赠送德国原装：可高温高压消毒的吸头盒（内置96个吸头）1 个；移液器支架1个；PP带盖试剂池1 个；吸头盒装填架1个；5．Viton密封环，硅油及安装工具1套。
1．产品性能,即移液器的准确性和重复性对于绝大多数用户而言,购买之前检测产品性能既有难度又无必要.因此,主要还是依据制造厂商提供的技术数据.但在这里还是要说明两点：其一,不要轻易相信卖家的口头承诺,一定要查阅制造商提供的书面材料；其二,在全球移液器市场上影响较大的品牌,如SOCOREX,EPPENDORF和GILSON等,其提供的技术数据可信度更高.
2．产品的可靠耐用这一方面,主要取决于移液器所用的材料.对于外壳,应当有较高的耐冲击性、耐腐蚀性和较低的导热性（如PVDF材质）；对于活塞,目前市场上主要有不锈钢、陶瓷和塑料三种材质.不锈钢机械性能好、寿命长,只是不太适合用于强酸强碱的移液；陶瓷则有很高的耐腐蚀性,但机械性能较差.当然,优质的材料往往意味着更高的价格,所以需要综合考虑购买价格和寿命的因素.
3．产品的人体工程学设计主要可以考虑以下几点：
第一,完成一个移液循环拇指的移动距离短,意味着舒适度更高；
第二,比较相同量程的移液器完成一次排液（一定要按到底）所需的拇指用力,这是影响舒适性的关键,用力越少意味着长期使用造成手指损伤的风险越小；
第三,装卸吸头,同样是越省力越好； 第四,移液器的重量适中,过重会增加手的负担,但过轻也往往意味着材质可能差强人意； 第五,其它的辅助设计,如壳体的磨砂设计以及指钩设计,有助于进一步提高舒适性.
国产移液器市场占有率高，以物美价廉占据市场。其次国产移液器有北京的金花移液器，整体构造仿造吉尔森移液器。其次就是一些打着国外移液器品牌，其实生产工厂大多在浙江和上海，以完全模仿国外或国产品牌为主。
进口品牌：艾本德、吉尔森移液器一直以来以良好的精准度和重复性被用户所青睐，艾本德新款移液器因为设计结构的问题，容易损坏。吉尔森移液器主要是人体工程学设计有所欠缺，吸液按钮比较硬，长时间使用容易产生疲劳感。
其次品牌：芬兰百得、美国瑞宁、德国普兰德、丹麦凯博、芬兰艾斯玛特、日本立洋等。
进口品牌产品每年都会涨价，特别是艾本德、吉尔森等一类品牌。现在移液器参数标准进口产品都没问题，贵在品牌和客户的青睐度。芬兰百得、美国瑞宁、德国普兰德也在向一类品牌移液器发展，价格直追一类品牌。
性价比高的进口品牌：
丹麦凯博、芬兰艾斯玛特、日本立洋等
所有移液器都可以用酒精或紫外灭菌处理，但要整支灭菌，其他的品牌就相对不占优势了。eppendorf research plus所有移液器都可以整支高温高压灭菌，这个是艾本德移液器的强势地方。
综合考虑在中国大陆地区选择Eppendorf多些，客户分应用反馈也是比较好的。建议购买艾本德移液器！
一、国产移液器我们以大龙移液器为例，它具有以下特点：
1. 重量轻，使用轻巧便捷，可减少手部疲劳。
2. 量程准确。
3. 量程具有锁定装置，符合人体手型和手感
4. 重量轻，使用轻巧便捷，可减少手部疲劳。
5. 具有可拆卸式高温高压消毒。
6. 产品规格齐全，共有16种。
二、进口移液器我们以德国艾本德移液器为例，它具有以下特点：
1. 符合ISO9000和GLP规定，用户可独立校准。
2. 十种规格，包括从0.1ul-5ml的体积变化，满足常规的需要，同时精度和误差较高。
3. 移液杆可360旋转,不用工具可卸下121℃高温灭菌。
4. 吸头接嘴有独特的V形圈，可以适合不同品牌的吸头。
5. 十种规格，包括从0.1ul-5ml的体积变化，满足常规的需要，同时精度和误差较高。
通过以上介绍，相信用户对这两种移液器的区别也有一定的了解了。
移液器使用过程中的注意事项如下：
一．设定移液体积：1.从大体积调节到小体积时，为正常调节方法，逆时针旋转刻度即可；2.从小体积调节至大体积时，可先顺时针调至超过设定体积的刻度，再回调至设定体积，这样可以保证最佳的精确度。
二．装配移液器吸头：1.单道移液器，将移液端垂直插入吸头，左右微微转动，上紧即可2.用移液器反复撞击吸头来上紧的方法是不可取的，这样操作会导致移液器部件因强烈撞击而松散，严重的情况会导致调节刻度的旋钮卡住。3.多道移液器，将移液器的第一道对准第一个吸头，倾斜插入，前后稍许摇动上紧，吸头插入后略超过O型环即可。
三．养护：1、如液体不小心进入活塞室应及时清除污染物；2、移液器使用完毕后，把移液器量程调至最大值，且将移液器垂直放置在移液器架上；3、根据使用频率所有的移液器应定期用肥皂水清洗或用60%的异丙醇消毒，再用双蒸水清洗并晾干；4、避免放在温度较高处以防变形致漏液或不准；5、发现问题及时找专业人员处理；6、当移液器吸嘴有液体时切勿将移液器水平或倒置放置，以防液体流入活塞室腐蚀移液器活塞；7、正确使用移液器吸液、排液，以达高精准度；8、平时检查是否漏液的方法：吸液后在液体中停1-3秒观察吸头内液面是否下降；如果液面下降首先检查吸头是否有问题，如有问题更换吸头，更换吸头后液面仍下降说明活塞组件有问题，应找专业维修人员修理。9、需要高温消毒的移液器应首先查阅所使用的移液器是否适合高温消毒后再行处理。
从大量程调节至小量程为正常调节方法，逆时针旋转刻度即可。从小量程调节至大量程时，应先调至超过设定体积刻度，再回调至设定体积，这样可以保证移液器的精确度
2．装配移液枪头
将移液枪垂直插入吸头，左右旋转半圈，上紧即可。用移液器撞击吸头的方法是非常不可取的，长期这样操作回导致移液器的零件因撞击而松散，严重会导致调节刻度的旋钮卡住
3．吸液及放液，垂直吸液吸头尖端浸入液面3mm以下，吸液前枪头先在液体中预润洗慢吸慢放放液时如果量很小则应吸头尖端可靠容器内壁
4．吸有液体的移液枪不应平放，枪头内的液体很容易污染枪内部而可能导致枪的弹簧生锈
5．移液枪在每次实验后应将刻度调至最大，让弹簧回复原型以延长移液枪的使用寿命
6.吸取液体时一定要缓慢平稳地松开拇指，绝不允许突然松开，以防将溶液吸入过快而冲入取液器内腐蚀柱塞而造成漏气。
7.为获得较高的精度，吸头需预先吸取一次样品溶液，然后再正式移液，因为吸取血清蛋白质溶液或有机溶剂时，吸头内壁会残留一层”液膜”，造成排液量偏小而产生误差。
8.浓度和粘度大的液体，会产生误差，为消除其误差的补偿量，可由试验确定，补偿量可用调节旋钮改变读数窗的读数来进行设定。
9.可用分析天平称量所取纯水的重量并进行计算的方法，来校正取液器，1mL 蒸馏水20℃时重0.9982g. 所设量程在移液器量程范围内不要将按钮旋出量程，否则会卡住机械装置，损坏了移液器。
10在设置量程时，请注意：数字清清楚楚在显示窗中， 旋转到所需量程
11.移液器严禁吸取有强挥发性、强腐蚀性的液体（如浓酸、浓碱、有机物等）。
12.严禁使用移液器吹打混匀液体。
13.不要用大量程的移液器移取小体积的液体，以免影响准确度。同时，如果需要移取量程范围以外较大量的液体，请使用移液管进行操作。
一般来说，为防止所吸取体积上出现误差，有一些基本的操作原则必须遵守。对吸取体积误差影响的因素主要有三个方面：①流体静压；②吸头润湿；③流体动力学。当样本体积从毫升范围降低至微升范围时，物理作用力的关系即发生变化，对于加样来说，其意味着液体表面的作用力效应与其体积或质量(例如重力)的作用力效应相比有所增加，因此，加样器生产厂家在设计和构建加样器和吸头中必须仔细考虑这种情况，而且在使用时也必须注意。
流体静压：在吸取液体时，加样器吸头只能浸入液体几毫升以确保与排出液体时相同的流体静压条件，因此，加样器必须以几乎垂直的方式加取液体，因为倾斜的方式将减少液体柱的高度，导致吸取的液体过多。如果加样器在30℃下以垂直方式吸取液体，可吸取至0．15％更多的液体。
吸头润湿：当吸头排空时，仍会有一些残留的液体以薄膜6f形式保留在吸头的侧面，其量取决于液体和吸头表面的相互作用，因此，其是一个常数，但依液体材料的不同而不同。对于水溶液，这种润湿影响在构建加样器时就应考虑。对于蛋白溶液等黏度高的液体，建议在加样前吸打液体数次，以保证加样的一致性。
流体动力学：对体积吸取的第三个影响是从加样器吸头外壁液体的释放，在此过程中，吸头的几何形状起一个关键的作用。为确保加样中的稳定条件，加样器吸头应靠在管壁上，于是，液体可顺着管壁流出，而不出现液滴，液滴的形成可由于其表面张力的作用而阻止液体从吸头中释放。如果吸头安装不正确或使用不相配的吸头，相对加样误差将达到0．4％以上。
