QuantideX® NGS DNA Hotspot 21 Kit
The QuantideX® NGS DNA Hotspot 21 Kit (RUO) is a next generation sequencing (NGS) research tool that interrogates 46 hotspot regions (amplicons) within 21 genes that are commonly mutated in a number of solid and hematological malignancies. The kit detects over 1,500 known variants, including single nucleotide variants (SNVs), insertions/deletions (indels), and structural rearrangements. Leveraging our proprietary NGS-in-a-Box™ workflow and Sample-Aware™ bioinformatics quality control solutions, this kit provides a simple, robust, and reliable NGS assay for the routine investigation of these genomic variants.
Features & Benefits
The QuantideX NGS DNA Hotspot 21 Kit combines Asuragen’s unique NGS-in-a-Box™ solution with a streamlined testing workflow to enable unprecedented NGS workflow efficiency and high sensitivity at low input amounts from precious FFPE samples.
Reduced ComplexityAssay incorporates sample-to-data solutions in a unique NGS-in-a-Box™ configuration
- Detects >1,500 variants from commonly mutated genomic regions across multiple tumor types
- End-to-end kitted solution
- Fully integrated data analysis pipeline
Optimized WorkflowProvides operational efficiencies to reduce testing costs, hands-on and total turnaround time
- Reduced labor required for library preparation
- Improved turnaround time enables higher throughput
- Common workflow across portfolio streamlines training & implementation
Quality PerformanceHighly sensitive assay with integrated, Sample-Aware™ bioinformatics software and built-in quality checks to minimize erroneous results and sample failures
- Highly sensitive detection of DNA-based variants
- Low input (~20ng) of DNA from FFPE
- Sample-Aware™ bioinformatics analysis and sample quality control
Product Description
Relevant ContentThe QuantideX NGS DNA Hotspot 21 Kit detects over 1,500 genomic variants as reported in the Catalogue of Somatic Mutations in Cancer (COSMIC) Database within 21 genes, representing approximately 80% of known variants within these targets.
Table 1: Mutation coverage of QuantideX NGS DNA Hotspot 21 Kit
Table 2: Clinical relevance of covered mutations
1NCCN Clinical Practice Guidelines*Includes gastric, pancreatic, glicomas, sarcomas, and other tumor types
A Fully Integrated WorkflowA unique NGS-in-a-Box™ configuration offers a simplified and fully integrated NGS workflow with cGMP-manufactured reagents, components and controls ready to use.
Includes:
- An internal quality control kit to measure the absolute copy number of amplifiable DNA and reports PCR inhibition
- Gene-specific PCR primers and Master Mix reagents
- Dual-index barcodes for sample multiplexing
- Library purification and quantification reagents
- Integrated data analysis and reporting software (QuantideX NGS Reporter)
Rapid & EfficientAdopt and run NGS-based analysis with minimum investment of time and resources, regardless of NGS experience and infrastructure.
- Fully integrated workflow reduces complexity and ensures reliable reagent quality
- Push-button analysis & reporting makes bioinformatics easy and efficient, regardless of experience level
- Integrated kit reduces overall workflow time
Performance Data
Highly Sensitive and Accurate Detection of DNA Mutations
QuantideX® NGS Reporter
Push-button analytics & reporting suite – Set-up-and-go workflow designed for easy installation and implementation, right out of the box.
Operates on a standard desktop computer – Install locally on a desktop computer using Windows® operating system. No prior bioinformatics experience or large server environments required.
Comprehensive– Full bioinformatics and reporting of variants (SNVs, Indels), and standard QC metrics are automatically calculated.
Integrates Sample-Aware™ – Bioinformatics with integrated functional template copy number analysis dramatically reduces false-call rates.
Resources
Videos
Next-Generation Sequencing Within Your Reach: Implementation of an Actionable Mutation Panel for Molecular Oncology Testing
Posters
Analytical Validation Of The QuantideX® NGS DNA Hotspot 21 Kit, A Diagnostic NGS System for the Detection of Actionable Mutations in FFPE TumorsView full poster
A Machine-Learning Framework for Accurate Classification and Quantification of Oncogenic Variants Using the QuantideX® NGS DNA Hotspot 21 KitView full poster
A Simple and Versatile Next-Generation Sequencing Technology for Co-Detection of RNA Structural Variants and DNA Mutations in Lung CancerView full poster
Ordering
| Product Name | Number of Reactions | Catalog Number |
|---|---|---|
| QuantideX NGS DNA Hotspot 21 Kit* | 48 | 46108 |
T 1.877.777.1874 | 512.681.5200 F 512.681.5202 E orders@asuragen.com
*For Research Use Only. Not for use in Diagnostic procedures.
QuantideX® NGS DNA Hotspot 21 Kit
The QuantideX® NGS DNA Hotspot 21 Kit is an in vitro diagnostic, next-generation sequencing (NGS) panel for the detection of clinically relevant variants across a multitude of tumor types, including non-small cell lung cancer, colorectal cancer, and melanoma. The kit screens for over 1,500 known genomic variants, including single nucleotide variants (SNVs), insertions/deletions (indels), and structural rearrangements, many of which are treatable with novel therapies, inform on patient management, or are the subject of further clinical evaluation. Leveraging our proprietary NGS-in-a-Box™ workflow and Sample-Aware™ bioinformatics quality control solutions, the panel provides a robust and reliable NGS solution for the identification of clinically relevant targets you and your clinicians can trust.
Features & Benefits
The QuantideX NGS DNA Hotspot 21 Kit combines Asuragen’s unique NGS-in-a-Box™ solution with a streamlined testing workflow to enable unprecedented NGS workflow efficiency and high sensitivity at low input amounts from precious FFPE samples.
Reduced ComplexityAssay incorporates sample-to-data solutions in a unique NGS-in-a-Box™ configuration
- Detects >1,500 variants from commonly mutated genomic regions across multiple tumor types
- End-to-end kitted solution
- Fully integrated data analysis pipeline
Optimized WorkflowProvides operational efficiencies to reduce testing costs, hands-on and total turnaround time
- Reduced labor required for library preparation
- Improved turnaround time enables higher throughput
- Common workflow across portfolio streamlines training & implementation
Quality PerformanceHighly sensitive assay with integrated, Sample-Aware™ bioinformatics software and built-in quality checks to minimize erroneous results and sample failures
- Highly sensitive detection of DNA-based variants
- Low input (~20ng) of DNA from FFPE
- Sample-Aware™ bioinformatics analysis and sample quality control
Product Description
Relevant ContentThe QuantideX NGS DNA Hotspot 21 Kit detects over 1,500 genomic variants as reported in the Catalogue of Somatic Mutations in Cancer (COSMIC) Database within 21 genes, representing approximately 80% of known variants within these targets. Several of these variants are associated with approved therapies, while others are currently in trials to clarify their clinical significance.
Table 1: Mutation coverage of QuantideX NGS DNA Hotspot 21 Kit
Table 2: Clinical relevance of covered mutations
1NCCN Clinical Practice Guidelines*Includes gastric, pancreatic, glicomas, sarcomas, and other tumor types
A Fully Integrated WorkflowA unique NGS-in-a-Box™ configuration offers a simplified and fully integrated NGS workflow with cGMP-manufactured reagents, components and controls ready to use.
Includes:
- An internal quality control kit to measure the absolute copy number of amplifiable DNA and reports PCR inhibition
- Gene-specific PCR primers and Master Mix reagents
- Dual-index barcodes for sample multiplexing
- Library purification and quantification reagents
- Integrated data analysis and reporting software (QuantideX NGS Reporter)
Rapid & EfficientAdopt and run NGS-based analysis with minimum investment of time and resources, regardless of NGS experience and infrastructure.
- Fully integrated workflow reduces complexity and ensures reliable reagent quality
- Push-button analysis & reporting makes bioinformatics easy and efficient, regardless of experience level
- Integrated kit reduces overall workflow time
Performance Data
Highly Sensitive and Accurate Detection of DNA Mutations
QuantideX® NGS Reporter
Push-button analytics & reporting suite – Set-up-and-go workflow designed for easy installation and implementation, right out of the box.
Operates on a standard desktop computer – Install locally on a desktop computer using Windows® operating system. No prior bioinformatics experience or large server environments required.
Comprehensive – Full bioinformatics and reporting of variants (SNVs, Indels) and standard QC metrics are automatically calculated.
Integrates Sample-Aware™ – bioinformatics with integrated functional template copy number analysis dramatically reduces false-call rates.
Resources
Videos
Next-Generation Sequencing Within Your Reach: Implementation of an Actionable Mutation Panel for Molecular Oncology Testing
Posters
Analytical Validation Of The QuantideX® NGS DNA Hotspot 21 Kit, A Diagnostic NGS System for the Detection of Actionable Mutations in FFPE TumorsView full poster
A Machine-Learning Framework for Accurate Classification and Quantification of Oncogenic Variants Using the QuantideX® NGS DNA Hotspot 21 KitView full poster
A Simple and Versatile Next-Generation Sequencing Technology for Co-Detection of RNA Structural Variants and DNA Mutations in Lung CancerView full poster
Ordering
| Product Name | Number of Reactions | Catalog Number |
|---|---|---|
| QuantideX NGS DNA Hotspot 21 Kit* | 48 | 76044 |
T 1.877.777.1874 | 512.681.5200 F 512.681.5202 E orders@asuragen.com
*For Research Use Only. Not for use in Diagnostic procedures.
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换算
1微升 = 0.000 000 001立方米
1微升 = 0.000 001 立方分米
1微升 = 0.000 001 升
1微升 = 0.001 毫升
1微升 = 0.001 立方厘米
1微升 = 1 立方毫米
1微升 = 1 000 纳升
如题，比如1000微升的枪，最大量程是1000，最大值要更大一些，一直用枪也没有注意过这个问题，记得原来上课老师讲是要调到最大量程，为了是弹簧处于松弛状态，那最大值应该才是最松弛状态吧，百度了一下也是有人说最大值，有人说最大量程，有人说没啥影响，想问问各位大神对这个小问题的理解，谢谢
移液器又称移液枪，是一种用于定量转移液体的器具，被广泛用于生物、化学等领域。
1.使用合适的吸头：
为确保更好的准确性和精度，建议移液量在吸头的35％-100％量程范围内。
2.吸头的安装：
对于大多数品牌的移液器，特别是多道移液器，安装吸头并非易事：为追求良好的密封性，需要将移液套柄插入吸头后，左右转动或前后摇动用力上紧。也有人会用移液器反复撞击吸头来上紧，但这样操作会导致吸头变形而影响精度，严重的则会损坏移液器，所以应当避免出现这样的操作。RAININ（瑞宁）的多道移液器没有O型环，配合有前挡点的吸头，只需轻压一下即可达致理想密封，实在是多道移液器使用者的福音。
3.吸头浸入角度和深度：
吸头浸入角度控制在倾斜20度之内，保持竖直为佳；吸头浸入深度建议如下所示：
移液器规格
吸头浸入深度
2µL和10 µL
1 mm
20µL和100 µL
2-3 mm
200µL和1000 µL
3-6 mm
5000 µL和10 mL
6-10 mm
4.吸头润洗：
对常温样品，吸头润洗有助于提高准确性；但是对于高温或低温样品，吸头润洗反而降低操作准确性，请使用者特别注意。
5.吸液速度：
移液操作应保持平顺、合适的吸液速度；过快的吸液速度容易造成样品进入套柄，带来活塞和密封圈的损伤以及样品的交叉污染。
建议：
1、移液时保持正确的姿势；不要时刻紧握移液器，使用带指钩的移液器帮助缓解手部疲劳；有可能的话经常换手操作。
2、定期检查移液器的密封状况，一旦发现密封老化或出现漏液，须及时更换密封圈。
3、每年对移液器进行1-2次校正（视使用频率而定）。
4、绝大多数移液器，在使用前和使用一段时间后，要给活塞涂上一层润滑油以保持密封性；而对于RAININ常规量程的移液器，不涂润滑油也同样拥有理想的密封性。
如仍有疑问，欢迎向企业知道提问。
一、国产移液器我们以大龙移液器为例，它具有以下特点：
1. 重量轻，使用轻巧便捷，可减少手部疲劳。
2. 量程准确。
3. 量程具有锁定装置，符合人体手型和手感
4. 重量轻，使用轻巧便捷，可减少手部疲劳。
5. 具有可拆卸式高温高压消毒。
6. 产品规格齐全，共有16种。
二、进口移液器我们以德国艾本德移液器为例，它具有以下特点：
1. 符合ISO9000和GLP规定，用户可独立校准。
2. 十种规格，包括从0.1ul-5ml的体积变化，满足常规的需要，同时精度和误差较高。
3. 移液杆可360旋转,不用工具可卸下121℃高温灭菌。
4. 吸头接嘴有独特的V形圈，可以适合不同品牌的吸头。
5. 十种规格，包括从0.1ul-5ml的体积变化，满足常规的需要，同时精度和误差较高。
通过以上介绍，相信用户对这两种移液器的区别也有一定的了解了。
