Human CD27 is a lymphocyte specific member of the tumor necrosis factor (TNF) receptor family and is found primarily on peripheral blood T cells and on a subpopulation of B cells and NK cells. The ligand for CD27 is CD70, which is a member of the TNF ligand superfamily (2, 3). Antibody M-T271 does not block the interaction between CD27 and CD70.

Isotype: Murine IgG1

Immunogen: T cell line (from patient with CLL)

Specificity: Antibody M-T271 recognizes the dimeric CD27 molecule of approximately 110 kd (1, 4) but does not block the interaction between CD70 and CD27. Clone M-T271 has been shown to abrogate IL-4 production(7) or induce apoptosis(6) in MLR . Clone M-T271 is crossreactive with cynomolgus monkey CD27 (8).

References:

1. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 350-352.

2. K. Agematsu, et al, (1994) J Immunol 153: 1421-1429.

3. R.Q. Hintzen, et al, (1994) Immunol Today 15: 307-311.

4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 356-360, 435-437.

5. K. Agematsu, et al, (1995) J Immunol 154: 3627-3635.

6. Wischhusen J, M Weller, et al. (2002) Cancer Res 62: 2592-2599

7. Palmer DR, U Krzych. Eur J Immunol 32(3): 652-661.

8. Yoshino N, Honda M, et al. (2000) Exp Anim 49(2):97-110.