| Liproxstatin-1 HClA potent ferroptosis inhibitor |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
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Science.2016 Aug 5;353(6299)594-8Nat Nanotechnol.2017 Dec;12(12):1190-1198.Nature Biotechnology.2017 Jun;35(6):569-576Nat Med.2018 Sep 17.Cell.2018 Dec 21. pii: S0092-8674(18)31561-7.Cell.Available online 25 October 2018.Cell.2018 Sep 27. pii: S0092-8674(18)31183-8.Cell.2018 Jun 28;174(1):172-186.e21.Cell.2018 Feb 22;172(5):1007-1021.e17.Cell.2017 Nov 30;171(6):1284-1300.e21.Cell.2017 Aug 17. pii: S0092-8674(17)30869-3.Cell.2017 Jul 13;170(2):312-323Nat Med.2018 Jan 29.Nat Med.2017 Nov;23(11):1342-1351.Cell.2017 Apr 6;169(2):286-300.Cell.2015 Aug 27;162(5):987-1002.Cell.2015 Feb 12;160(4):729-44.Nature Medicine.2017 Apr;23(4):493-500.Cancer Cell.2018 May 14;33(5):905-921.e5.Cancer Cell.2018 Apr 9;33(4):752-769.e8.Cancer Cell.2018 Mar 12;33(3):401-416.e8.Cancer Cell.2017 Aug 14;32(2):253-267.e5.Nat Methods.2018 Jul;15(7):523-526.Cell Stem Cell.2018 May 3;22(5):769-778.e4.Cell Stem Cell.2017 Nov 20. pii: S1934-5909(17)30375-2.Quality Control & MSDS
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- Purity = 98.82%
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Chemical structure
| Cell experiment [1]: | |
Cell lines | Gpx4-/- cells |
Preparation method | The solubility of this compound in DMSO is > 10.5 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
Reacting condition | 72 hrs |
Applications | Liproxstatin-1 HCl inhibited the growth of Gpx4-/- cells with an IC50 value of 22 nM. At the dose of 50 nM, Liproxstatin-1 HCl completely prevented lipid peroxidation. Liproxstatin-1 HCl (200 nM) dose-dependently protected Gpx4-/- cells against ferroptosis-inducing agents, such as L-buthionine sulphoximine (10 μM), erastin (1 μM) and RSL3 (0.5 μM), whereas it failed to rescue cell death caused by staurosporine (0.2 μM) and H2O2 (200 μM). |
| Animal experiment [1]: | |
Animal models | GreERT2; Gpx4fl/fl mice |
Dosage form | 10 mg/kg; i.p. |
Applications | In GreERT2; Gpx4fl/fl mice, Liproxstatin-1 HCl significantly extended the survival period. The TUNEL staining results after 9-day treatment showed a markedly reduced number of TUNEL+ cells in the Liproxstatin-1 HCl treatment group than in the vehicle control group, indicating that Liproxstatin-1 HCl delayed ferroptosis in tubular cells. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1]. Friedmann Angeli JP, Schneider M, Proneth B, et al. Inactivation of the ferroptosis regulator Gpx4 triggers acute renal failure in mice. Nat Cell Biol, 2014, 16(12): 1180-1191. | |
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| Cas No. | N/A | SDF | Download SDF |
| Synonyms | N/A | ||
| Chemical Name | N-(3-chlorobenzyl)-4"H-spiro[piperidine-4,3"-quinoxalin]-2"-amine hydrochloride | ||
| Canonical SMILES | ClC1=CC(CNC(C2(CCNCC2)N3)=NC4=C3C=CC=C4)=CC=C1.Cl | ||
| Formula | C19H22Cl2N4 | M.Wt | 377.31 |
| Solubility | ≥18.85mg/mL in H2O | Storage | Store at -20°C |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Liproxstatin-1 HCl is a potent inhibitor of ferroptosis with IC50 value of 22 nM [1].
Ferroptosis is an iron-dependent non-apoptotic form of cell death induced by small molecules in engineered cells overexpressing oncogenic RAS and specific tumour types [1].
Liproxstatin-1 HCl is a potent ferroptosis inhibitor. Liproxstatin-1 HCl showed inhibition against ferroptosis-inducing agent (FIN) -triggered cell death. In Gpx4-/- cells, Liproxstatin-1 HCl inhibited RSL3-induced BODIPY 581/591 C11 oxidation. In primary human renal proximal tubule epithelial cells (HRPTEpiCs), Liproxstatin-1 HCl inhibited RSL3-induced cell death. Knock down Gpx4 in the immortalized human renal proximal tubule epithelial cell line (HK-2) cells made cells more sensitive to FIN [1].
On TAM treatment of CreERT2; Gpx4fl/fl mice, Liproxstatin-1 HCl significantly extended survival time and reduced number of TUNELC cells, which suggested that Liproxstatin-1 HCl delayed ferroptosis in tubular cells. In a hepatic ischaemia/reperfusion injury model, Liproxstatin-1 HCl mitigated tissue injury induced by ischaemia/reperfusion [1].
Reference:[1]. Friedmann Angeli JP, Schneider M, Proneth B, et al. Inactivation of the ferroptosis regulator Gpx4 triggers acute renal failure in mice. Nat Cell Biol, 2014, 16(12): 1180-1191.
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电压等于电位差。参考点不同则各点电位值不同，但是各点之间的电压不变。向左转|向右转如图，a 是参考点，电位 Va = 0 ，Vb = E1 ，Ve = - E2 。电压 U1 = Vb - Vc ，U2 = Vc - Vd 。
不要拿树上概念糊弄我，半个专业的。
参考资料：http://baike.baidu.com/search/word?word=%E7%94%B5%E4%BD%8D
电压（voltage），也称作电势差或电位差，是衡量单位电荷在静电场中由于电势不同所产生的能量差的物理量。其大小等于单位正电荷因受电场力作用从A点移动到B点所做的功，电压的方向规定为从高电位指向低电位的方向。电压的国际单位制为伏特（V，简称伏），常用的单位还有毫伏（mV）、微伏（μV）、千伏（kV）等。此概念与水位高低所造成的“水压”相似。需要指出的是，“电压”一词一般只用于电路当中，“电势差”和“电位差”则普遍应用于一切电现象当中。
参考资料：http://baike.baidu.com/view/10954.htm
希望我的回答能够帮助到你，望采纳，谢谢。
我是苏州大学心血管内科研究生，我们打算使用电压敏感染料膜电位光学标测技术做体外心肌细胞的实验。但是目前实验室没人做过，想上门拜师学习！谢谢！~~~
我的联系方式：15962175904姓名：林欢单位：苏州大学医学院
电位：是电路里某点对参考点之间的电压，而参考点的电位一般规定为0伏。
电位是相对的，电路中某点电位的大小，与参考点的选择有关；选择不同的参考点，电位的值是不一样的。
这个概念跟高度有点类似;
比如说这栋楼房楼高9米，就是指它离地面的高度，地面就是参考点，地面的高度为0 。
而第2层楼有3米高，就是指第3层高度减去第1层的高度。
问题1，钠通道的大量激活，是只有在达到阈刺激时才能大量激活还是达到一个特定电位（不管骨骼肌还是心肌这个特定电位不变）才能激活？
问题2，激活是一个瞬态，瞬间大量激活，瞬间大量失活，那这个动作电位上升支持续的时间怎么解释？
问题3，钠通道的失活应该也是电压依赖性的，导致失活电压是特定的么？在骨骼肌和心肌一样么？
问题4，在从静息电位到阈电位这一段时间，第106页心肌的图和第39页骨骼肌的图一个兴奋性一个不变一个兴奋性增大怎么解释？
问题5，在第106页的第2个图上在RRP和SNP时期引出动作电位，动作电位的上升支不是应该接近钠离子的平衡电位才终止么，而这个平衡电位应该只跟细胞内外钠离子的浓度变化有关，但动作电位导致的细胞内外钠离子的浓度变化应该不大，怎么会使出现几个动作电位的上升的最高点是不一样的呢？
问题6，怎么理解钠离子通道的时间依赖性？33页的图怎么看？为什么随着电压增大，钠电导增大呢？不是只有在阈刺激时钠通道才大量开放，这个时候才应该钠电导最大么？
最近在学习电生理，有些问题不太明白，还望大家不吝指教！
在电压钳-65mv下记录到的spike是不是跟在电流钳下记录到的动作电位一致，另外有人认为在没有动作电位电流即spike的情况下，依然能够记录到自发性动作电位，那么这个自发性动作电位是怎么产生的
