Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 50476 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
| AAV5 | 50476-AAV5 | Virus (100 µL at titer ≥ 2×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV8 | 50476-AAV8 | Virus (100 µL at titer ≥ 3×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV(Search Vector Database)
- Backbone sizew/o insert(bp)4818
- Total vector size (bp)7886
- Vector typeAAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)NEB Stable
- Copy numberLow Copy
Gene/Insert
- Gene/Insert namehM3D(Gq)-mCherry
- SpeciesH. sapiens (human)
- Insert Size (bp)2502
- MutationSee supplemental documents for DREADD mutations
- Entrez GeneCHRM3(a.k.a. EGBRS, HM3, PBS)
- PromoterCaMKIIa
- Tag/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning methodRestriction Enzyme
- 5′ cloning siteSal I(not destroyed)
- 3′ cloning siteEcoR I(not destroyed)
- 5′ sequencing primerATGCTGACGAAGGCTCGCGA
- 3′ sequencing primerGCATTAAAGCAGCGTATCCACATAGC (Common Sequencing Primers)
Resource Information
- Supplemental Documents
- DREADD mutations
- Terms and Licenses
- UBMTA
- Takara Bio Limited Use Label License (formerly Clontech)
- Industry Terms
- Not Available to Industry
- Articles Citing this Plasmid
- 3 References
Depositor Comments
For more information, see: http://pdspit3.mml.unc.edu/projects/dreadd/wiki/WikiStart
Information for AAV5 (Catalog # 50476-AAV5)(Back to top)
Purpose
Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA.
CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 2×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV5
- PurificationCsCl gradient ultracentrifugation
- Reporter GenemCherry
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Addgene Comments
Data submitted about 50476-AAV5 by requesting scientist(s):
- Data 1: Rat, Brain parenchyma
Information for AAV8 (Catalog # 50476-AAV8)(Back to top)
Purpose
Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA.
CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 3×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV8
- PurificationIodixanol gradient ultracentrifugation
- Reporter GenemCherry
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
最近在学习电生理,有些问题不太明白,还望大家不吝指教!
在电压钳-65mv下记录到的spike是不是跟在电流钳下记录到的动作电位一致,另外有人认为在没有动作电位电流即spike的情况下,依然能够记录到自发性动作电位,那么这个自发性动作电位是怎么产生的
我是苏州大学心血管内科研究生,我们打算使用电压敏感染料膜电位光学标测技术做体外心肌细胞的实验。但是目前实验室没人做过,想上门拜师学习!谢谢!~~~
我的联系方式:15962175904姓名:林欢单位:苏州大学医学院
假如A点的电位是10V,B点的电位是8V,C点的电位是6V.那么A到B之间的电压差是2V,A到C之间的电压是4V.
通俗点讲:我们把两节干电池串联,接个小灯泡,小灯泡两端的电压差就是3V,而一节干电池的两端的电压差是1.5V.如果我们将两节干电池并联,再接个小灯泡,小灯泡两端的电压差就是1.5V(并接电池的作用是加大容量,这个估计你还不懂)
电压差就是用高电位减去低电位的差值.设干电池的正极为1.5V,负极为0V,两点的电压差就是1.5V.设干电池的正极为0V,负极就为-1.5V.当两节干电池串联的时候,一节的负极与另外一节的正极连在一起,他们就是等电位,都是0V,所以第一节的正极1.5V减去第二节的负极-1.5V就得出从第一节的正极,到第二节的负极的电压差为3V.
更通俗的说:电位就相当于你现在所在的楼层,比如你现在在5楼,另外有个电位是在二楼,你和他就相差3层楼(这就是电压差),如果另外一个电位在地下1层,那你和他就相差6层(这个也是电压差)
电压等于电位差。参考点不同则各点电位值不同,但是各点之间的电压不变。向左转|向右转如图,a 是参考点,电位 Va = 0 ,Vb = E1 ,Ve = - E2 。电压 U1 = Vb - Vc ,U2 = Vc - Vd 。
参考资料:http://baike.baidu.com/search/word?word=%E7%94%B5%E4%BD%8D
电压(voltage),也称作电势差或电位差,是衡量单位电荷在静电场中由于电势不同所产生的能量差的物理量。其大小等于单位正电荷因受电场力作用从A点移动到B点所做的功,电压的方向规定为从高电位指向低电位的方向。电压的国际单位制为伏特(V,简称伏),常用的单位还有毫伏(mV)、微伏(μV)、千伏(kV)等。此概念与水位高低所造成的“水压”相似。需要指出的是,“电压”一词一般只用于电路当中,“电势差”和“电位差”则普遍应用于一切电现象当中。
参考资料:http://baike.baidu.com/view/10954.htm
希望我的回答能够帮助到你,望采纳,谢谢。

