Type
Recombinant
Description
Total 220 AA. MW: 25 kDa (calculated). UniProtKB acc.no. Q9BY76. N-Terminal His-tag, 16 extra AA (highlighted).
Amino Acid Sequence
MRGSHHHHHHGMASHMGPVQSKSPRFASWDEMNVLAHGLLQLGQGLREHAERTRSQLSALERRLSACGSACQGTEGSTDLPLAPESRVDPEVLHSLQTQLKAQNSRIQQLFHKVAQQQRHLEKQHLRIQHLQSQFGLLDHKHLDHEVAKPARRKRLPEMAQPVDPAHNVSRLHRLPRDCQELFQVGERQSGLFEIQPQGSPPFLVNCKMTSDGGWTVIQR
Source
E. coli
Purity
>95%
SDS-PAGE Gel
12% SDS-PAGE separation of Human ANGPTL4
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 10μg/lane
3. non-reduced and non-heated sample,10μg/lane
Biological Activity
Effects of ANGPTL4 on serum levels of triglycerides, on blood glucose and on serum cholesterol levels in C57BL/6J mice.
Endotoxin
< 1.0 EU/ug
Formulation
Filtered (0.4 μm) and lyophilized from 0.5 mg/mL solution in 30 mM acetate buffer, pH 4.0
Reconstitution
Add 0.1 M acetate buffer pH 4.0 to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 μg/mL. In higher concentrations the solubility of this antigen is limited. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
Applications
Western blotting, ELISA, Biologically active protein
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
Note
This product is intended for research use only.
