Type
Recombinant
Description
Proteases(alsocalledProteolyticEnzymes,Peptidases orProteinases)areenzymesthathydrolyzetheamidebondswithinproteinsorpeptides.Mostproteasesactinaspecificmanner,hydrolyzingbondsatoradjacenttospecificresiduesoraspecificsequenceofresiduescontainedwithinthesubstrateproteinorpeptide.ProteasesplayanimportantroleinmostdiseasesandBIOLOGicalprocessesincludingprenatalandpostnataldevelopment,reproduction,signaltransduction,theimmuneresponse,variousautoimmuneanddegenerativediseases andcancer.Theyarealsoanimportantresearchtool,frequentlyusedintheanalysisandproductionofproteins.Furinisacalciumdependentserineendoproteasethatprocessesnumerousproproteinsofdifferentsecretorypathwaysintotheirmatureformsbycleavingatthecarboxylsideoftherecognitionsequence,R-Xaa-(K/R)-R,whereXaacanbeanyaminoacid.RecombinanthumanFurinisa61.7 kDaprotein,correspondingtoresidues124 through715 oftheFurinprecursorplusaC-terminalHis tag.
AminoAcidSequence
DLNVKAAWAQGYTGHGIVVSILDDGIEKNHPDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVANNGVCGVGVAYNARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPEDDGKTVDGPARLAEEAFFRGVSQGRGGLGSIFVWASGNGGREHDSCNCDGYTNSIYTLSISSATQFGNVPWYSEACSSTLATTYSSGNQNEKQIVTTDLRQKCTESHTGTSASAPLAAGIIALTLEANKNLTWRDMQHLVVQTSKPAHLNANDWATNGVGRKVSHSYGYGLLDAGAMVALAQNWTTVAPQRKIIDILTEPKDIGKRLEVRKTVTACLGEPNHITRLEHAQARLTLSYNRRGDLAIHLVSPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTKFTLVLYGTAPEGLPVPPESSGCKTLTSSQACVVCEEGFSLHQKSCVQHCPPGFAPQVLDTHYSTENDVETIRASVCAPCHACSATCQGPALTDCLSCPSHASLDPVEQTCSRQSQSSRESPPQQQPPRLPPEVEAGQRLRAGLLPSHLPEHHHHHHHH
Source
Hi-5Insectcells
Purity
95%
BiologicalActivity
MeasuredbyitsABIlitytocleavethefluorogenicpeptidesubstrateBoc-Arg-Val-Arg-Arg-AMC.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.
Storage/Expiration
–20°C