
- SynonymStreptavidin,SA
- SourceRecombinant Streptavidin (STN-N5116) is expressed from E.coli cells.
- ApplicationELISA assay
- Formulation
Lyophilized from 0.22 μm filtered solution in NaCl, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 2 years in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with a linear range of 0.1-6 ng/mL (QC tested)

Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).
- BackgroundStreptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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抗原:
(antigen,缩写Ag)为任何可诱发免疫反应的物质。外来分子可经过B细胞上免疫球蛋白的辨识或经抗原呈现细胞的处理并与主要组织相容性复合体结合成复合物再活化T细胞,引发连续的免疫反应。
抗体:
(英语:antibody),(免疫球蛋白不仅仅只是抗体)是一种由浆细胞(效应B细胞)分泌,被免疫系统用来鉴别与中和外来物质如细菌、病毒等的大型Y形蛋白质,仅被发现存在于脊椎动物的血液等体液中,及其B细胞的细胞膜表面[1-2]。抗体能识别特定外来物的一个独特特征,该外来目标被称为抗原。
抗原抗体反应:
指抗原与相应抗体之间所发生的特异性结合反应。这种反应既可在机体内进行,也可以在机体外进行。抗原抗体反应的过程是经过一系列的化学和物理变化,包括抗原抗体特异性结合和非特异性促凝聚两个阶段,以及由亲水胶体转为疏水胶体的变化。
体液免疫:生发中心母细胞的轻链和重链V基因可发生高频率的点突变,在抗原诱导的情况下产生.在初次免疫应答时,大量抗原的出现,可使表达不同亲和力的BCR的各种B细胞克隆被选择和激活,产生多种不同亲和力的抗体.每个B细胞开始时一般均表达IgM,在免疫应答时首先分泌IgM,通过重链C区的基因重排,随后会产生IgG,IgA,起主要的免疫应答作用.
细胞免疫:抗原被提呈给T细胞后,T细胞活化,产生大量的细胞因子,比如干扰素,白细胞介素等,参与细胞免疫过程.
由此,注射疫苗(没有破坏力但却能刺激机体产生对应的抗体)属于主动免疫,注射血清(即抗体)属于被动免疫,前者旨在预防,标本兼治,后者则能够应急。

