SDS-PAGE: SDS-PAGE showing 5µg purified Tetanus Toxoid (recombinant heavy chain fragment C), with and without DTT.

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TETANUS TOXOID, RECOMBINANT HEAVY CHAIN FRAGMENT C
Tetanus toxoid, recombinant heavy chain fragment C is a recombinant protein that is devoid of the toxin’s enzymatic activity but contains the binding site necessary for uptake of the toxin by neurons, and is the most immunogenic part of the toxin. Fragment C contains the powerful T cell epitopes of tetanus toxin without the toxicity.
PRODUCT DETAILS – TETANUS TOXOID, RECOMBINANT HEAVY CHAIN FRAGMENT C
- Carboxyl fragment of tetanus toxin heavy chain expressed in the cytoplasm of E. coli as a properly-folded, soluble protein.
- Purified in 20mM HEPES, l00mM NaCl, 10% glycerol, pH7.2.
- Manufactured to >98% purity by SEC HPLC.
BACKGROUND
TT heavy chain fragment is the C terminal fragment of the toxin (Helting & Zwisler, 1977). It is devoid of the toxin’s enzymatic activity but contains the binding site necessary for uptake of the toxin by neurons (Roux et al., 2015; Sinha et al., 2000) and is the most immunogenic part of the toxin (Ramakrishnan et al., 2015). Fragment C contains the powerful T cell epitopes of tetanus toxin without the toxicity.
Tetanus toxoid (TT) is frequently used as a carrier protein for conjugate vaccines, in addition to being a component of the DPT vaccine. TT contains strong T cell epitopes. However, as a toxoided protein, many of the surface lysines are blocked by the toxoiding process. Furthermore, TT is not a uniform product, since each manufacturer has its own specific toxoiding and purification process. Tetanus toxoid also tends to aggregate with age and is not generally affordable in the quantities needed for research and early clinical work. Recombinant TT combines the advantages of the toxoid with the reproducibility of a recombinant protein.
REFERENCES
- Helting and Zwisler. (1977). Structure of Tetanus Toxin: I. Breakdown of the Toxin Molecule. J Biol. Chem. 252:137, 1977.
- Roux et al. (2005). C-terminal fragment of tetanus toxin: its use in neuronal network analysis and its potential as non-viral vector. J Soc Biol. 199:35, 2005.
- Sinha et al. (2000). Analysis of mutants of tetanus toxin Hc fragment: ganglioside binding, cell binding and retrograde axonal transport properties. Mol Microbiol. 37:1041, 2000.
- Ramakrishnan et al. (2015). Utility of recombinant fragment C for assessment of anti-tetanus antibodies in plasma. Diagn Microbiol Infect Dis. 82:11, 2015.
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第三道防线是人体在出生以后逐渐建立起来的后天防御功能,特点是出生后才产生的,只针对某一特定的病原体或异物起作用,因而叫做特异性免疫(又称后天性免疫)。后天性免疫--免疫的第三道防线免疫的第三道防线:特异性免疫。主要由免疫细胞--淋巴B、淋巴T细胞进行免疫。其中,淋巴B细胞“负责”体液免疫;淋巴T细胞“负责”细胞免疫。后天性的特异性免疫系统,是一个专一性的免疫机制,针对一种抗原所生成的免疫淋巴细胞分泌的抗体,只能对同一种抗原发挥免疫功能。而对变异或其他抗原毫无作用。第1、2防线,就好比杀毒软件本体;第3到防线就好比病毒/木马专杀软件。只有3道防线同时、完整、完好发挥免疫作用,我们的身体健康才能更充分的得到保证。展开
抗原:
(antigen,缩写Ag)为任何可诱发免疫反应的物质。外来分子可经过B细胞上免疫球蛋白的辨识或经抗原呈现细胞的处理并与主要组织相容性复合体结合成复合物再活化T细胞,引发连续的免疫反应。
抗体:
(英语:antibody),(免疫球蛋白不仅仅只是抗体)是一种由浆细胞(效应B细胞)分泌,被免疫系统用来鉴别与中和外来物质如细菌、病毒等的大型Y形蛋白质,仅被发现存在于脊椎动物的血液等体液中,及其B细胞的细胞膜表面[1-2]。抗体能识别特定外来物的一个独特特征,该外来目标被称为抗原。
抗原抗体反应:
指抗原与相应抗体之间所发生的特异性结合反应。这种反应既可在机体内进行,也可以在机体外进行。抗原抗体反应的过程是经过一系列的化学和物理变化,包括抗原抗体特异性结合和非特异性促凝聚两个阶段,以及由亲水胶体转为疏水胶体的变化。
由此,注射疫苗(没有破坏力但却能刺激机体产生对应的抗体)属于主动免疫,注射血清(即抗体)属于被动免疫,前者旨在预防,标本兼治,后者则能够应急。