- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Proteoglycans are categorized depending upon the nature of their glycosaminoglycan chains (chondroitin sulfate, dermatan sulfate, heparan sulphate and keratan sulfate) as well as characterized by size.
Aggrecan is a large aggregating proteoglycan of articular cartilage. It is also found in aorta tissue, discs, tendons and in the perineuronal net. It is responsible for hydrating cartilage, giving it compressibility and resilience during joint loading, thereby playing a major role in the normal function of cartilage. Depletion of glycosaminoglycan-bearing aggrecan fragments is one of the earliest events in cartilage destruction.
Data/Specifications
Immunogen:Synthetic peptide: EPEEPFTFAPEI
Clone:6B4
Host: Mouse
Myeloma: x63-Ag8.653
Isotype: IgG1
Light Chain type: kappa
Specificity:Recognizes the linear amino acid sequence 394EPEEPFTFAPEI406 present in the interglobulardomain (IGD) of human and bovine aggrecan.
Cross-reactivity: This antibody cross-reacts with human and bovine
Purity: Affinity purified on protein G
Form: Liquid, 1 mL/vial
Concentration: 0.1 mg/mL
Storage: -20° C
Literature/Support
Aggrecan IGD Antibody Insert (PDF, 334 KB)
Joint Disease and Aggrecan (blog post)
How To Use
Applications
- ELISA: Use at an assay dependant dilution.
- IHC-P: Use at an assay dependant dilution.
- IHC-Fr: Use at an assay dependant dilution.
- WB: Use at a dilution of 1/100.
Technical Notes
Optimal dilutions/concentrations should be determined by the end user. This antibody
should work in IHC on formalin or paraformaldehyde-fixed paraffin embedded sections as
well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections.
Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01
Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG
of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.
Related Products
ebiomall.com
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抗原：
（antigen，缩写Ag）为任何可诱发免疫反应的物质。外来分子可经过B细胞上免疫球蛋白的辨识或经抗原呈现细胞的处理并与主要组织相容性复合体结合成复合物再活化T细胞，引发连续的免疫反应。
抗体：
（英语：antibody），（免疫球蛋白不仅仅只是抗体）是一种由浆细胞（效应B细胞）分泌，被免疫系统用来鉴别与中和外来物质如细菌、病毒等的大型Y形蛋白质，仅被发现存在于脊椎动物的血液等体液中，及其B细胞的细胞膜表面[1-2]。抗体能识别特定外来物的一个独特特征，该外来目标被称为抗原。
抗原抗体反应：
指抗原与相应抗体之间所发生的特异性结合反应。这种反应既可在机体内进行，也可以在机体外进行。抗原抗体反应的过程是经过一系列的化学和物理变化，包括抗原抗体特异性结合和非特异性促凝聚两个阶段，以及由亲水胶体转为疏水胶体的变化。
体液免疫：生发中心母细胞的轻链和重链V基因可发生高频率的点突变,在抗原诱导的情况下产生.在初次免疫应答时,大量抗原的出现,可使表达不同亲和力的BCR的各种B细胞克隆被选择和激活,产生多种不同亲和力的抗体.每个B细胞开始时一般均表达IgM,在免疫应答时首先分泌IgM,通过重链C区的基因重排,随后会产生IgG,IgA,起主要的免疫应答作用.
细胞免疫：抗原被提呈给T细胞后,T细胞活化,产生大量的细胞因子,比如干扰素,白细胞介素等,参与细胞免疫过程.
由此，注射疫苗（没有破坏力但却能刺激机体产生对应的抗体）属于主动免疫，注射血清（即抗体）属于被动免疫，前者旨在预防，标本兼治，后者则能够应急。
