Anti-PAK
GloboZymes Anti-PAK is an affinity-purified polyclonal rabbit antibody produced to a synthetic peptide corresponding to residues 484-502 of human PAK1. The antibody is useful for Western blotting. For example, the antibody may be used at 1:1000 dilution with 30 μg HEK293 cell extracts. In addition to PAK1, the antibody displays some reactivity with PAK2.
Synonyms: Anti-p65PAK; Anti-p68PAK; Antibody to p21-Activated Protein Kinase.
Figure: An extract of HEK293 cells was subjected to Western blotting with GloboZymes affinity-purified Anti-PAK polyclonal antibody. The arrow denotes the position of PAK65 (65 kDa).
Background: The p21-GTPase-activated protein kinases (PAK’s) are a family of enzymes which regulate numerous processes. These processes include cell morphology and cytoskeleton organization, cell motility, apoptosis, neuronal functions, cell cycle progression, and other processes. PAK’s interact with several important regulatory proteins including Protein Phosphatase 2A (PP2A), ribosomal protein S6 kinase, Mixed Lineage Kinase (MLK2), and the SH3-SH2 adapter Nck. Physiological targets of PAK include p38 MAP kinase, JNK, cRaf-1 and Synapsin I. The catalytic domain of PAK is produced in apoptotic cells via a caspase-dependent pathway. Unlike the native enzymes, the catalytic domain does not require Rac or Cdc42 to undergo autophosphorylation and activation.
References: Nature 367: 40; J Biol Chem 270: 21695
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各位好，最近构建慢病毒沉默载体，问题是提完质粒酶切验证是阳性，但是测序检测不到插进去的shRNA，已经重复连接转化多次，依旧没有得到阳性质粒，请大神帮忙分析下原因，感谢！！
例用微物测定孢克罗效价用孢克罗标准品用HPLC或UV测定则用照品；非西丁用作熔点校准物质用熔点标准品测定含量用照品即使同种物质标准品照品规格、标定及用途都能同（北京标准物质网）
n.[医] （对伤处等的） 针探，探查; [医] 探针，取样器; 探测仪;探头;
vt.探索，调查; 用探针（或探测器等）探查，探测;
vt.盘问; （用试探性袭击等）侦察（敌情） ; 用尖物刺穿（物件）; 用力使向前推进;
The more they probed into his background, the more inflamed their suspicions would become
他们越调查他的背景，疑团就越多。
请问大神，siRNA一定是瞬转；如果瞬转，比如只能维持个2-3天，那么如果我想观察药物A作用于siRNA干预的细胞的效果，但是这个药物A需要作用10天，那么我间隔2-3天加一点SIRNA，这样可以吗，谢谢大神了
①些物,RNA病毒没DNA,能用cDNA克隆；
②cDNA克隆易筛选,cDNA库包含非结构基克隆,且每cDNA克隆含mRNA信息；
③cDNA能细菌表达.cDNA仅代表某发育阶段表达遗传信息,基文库才包含物完整遗传信息.
