Name

2x Es Taq Master Mix (with dye)

Cat. #

Application

• Routine PCR
• T/A cloning

Specifications

This Master Mix contains Es Taq DNA Polymerase, PCR buffer, Mg2+, dNTP, PCR stabilizer and PCR enhancer.The concentration is 2x.

Es Taq DNA Polymerase possesses 5´→3´ DNA polymerase and 5´→3´ exonuclease activity. The polymerase has the high amplification efficiency and low mismatching as Taq DNA polymerase and high fidelity of Pfu DNA polymerase. Es Taq Polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for T/A cloning.The amplification range of Es Taq is ~ 6 kb.

This unique Master Mix recipe makes the system very reliable.More than 98% of PCR reaction can get successful amplification during the first try.It also works well on complicate templates.

The Master Mix contains dye, and can directly run electrophoresis after PCR reaction.

Shipping / Storage

Quality control

Manual (protocol)

PCR reaction system

Note: The recommended primer concentration for PCR is between 0.1-1.0 µM of each primer. The use of higher concentrations of primers can have higher amplification effect. Low primer concentration will generally ensure cleaner product and lower background.

PCR reaction conditions

Note:

• The recommended annealing temperature is about 5℃ below Tm of primers. If extra bands are observed, higher annealing temperatures should be considered. The absence of product indicates the need for a lower annealing temperature.
• PCR extension time is depended on the size of target gene sequence.Es Taq DNA polymerase is approximately 1 kb DNA / 30 seconds.
• The number of PCR cycles will basically depend on the downstream application of the PCR product.

This Master Mix contains dye for electrophoresis.After PCR, directly load 5 µL of PCR product to agarose gel to run electrophoresis.No need to add loading buffer.