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- Specification
- Product Description:
- This protein phosphorylation antibody pair set comes with two antibodies, one against the BAD protein, and the other against the specific S134 phosphorylated site of BAD for use in in situ Proximity Ligation Assay. See Publication Reference below.
- Reactivity:
- Human
- Quality Control Testing:
- Dual recognition immunofluorescence result.
Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
- Supplied Product:
- Antibody pair set content: 1. Phospho-BAD S134 rabbit polyclonal antibody (20 ul) With 0.09% sodium azide. 2. BAD mouse monoclonal antibody (40 ug) In 1x PBS, pH 7.2 *Reagents are sufficient for at least 30-50 assays using recommended protocols.
- Storage Instruction:
- Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
- Datasheet:
Download
- Publication Reference
- 1.
- In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method.Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, Andersson AC, Wahlby C, Gullberg M,Botling J,Sjoblom T, Markova B, Ostman A, Landegren U, Soderberg O.Mol Cell Proteomics. 2007 Sep;6(9):1500-9. Epub 2007 Jun 12.
- 2.
- Direct observation of individual endogenous protein complexes in situ by proximity ligation.Soderberg O, Gullberg M, Jarvius M , Ridderstrale K, Leuchowius KJ, Jarvius J, Wester K, Hydbring P, Bahram F, Larsson LG, and Landegren U. Nat Methods. 2006 Dec;3(12):995-1000. Epub 2006 Oct 29.
- 3.
- Cytokine detection by antibody-based proximity ligation.Gullberg M, Gustafsdottir SM, Schallmeiner E, Jarvius J, Bjarnegard M, Betsholtz C, Landegren U, andFredriksson S. Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8420-4. Epub 2004 May 21.
- 4.
- Protein detection using proximity-dependent DNA ligation assays.Fredriksson S, GullbergM, Jarvius J, Olsson C, Pietras K, Gustafsdottir SM, Ostman A, and Landegren U. Nat Biotechnol. 2002 May;20(5):473-7.
- 5.
- Highly specific detection of phosphorylated proteins by DuolinkMats Gullberg and Ann-Catrin Andersson Nature Methods 6. 2009
- Applications
- In situ Proximity Ligation Assay (Cell)
- Application Image
- In situ Proximity Ligation Assay (Cell)
- Gene Information
- Entrez GeneID:
- 572
- Gene Name:
- BAD
- Gene Alias:
- BBC2,BCL2L8
- Gene Description:
- BCL2-associated agonist of cell death
- Omim ID:
- 603167
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq
- Other Designations:
- BCL-X/BCL-2 binding protein,BCL2-antagonist of cell death protein,BCL2-binding component 6,BCL2-binding protein
- Gene Pathway
- Acute myeloid leukemia
- Alzheimer"s disease
- Amyotrophic lateral sclerosis (ALS)
- Apoptosis
- Chronic myeloid leukemia
- Colorectal cancer
- Endometrial cancer
- ErbB signaling pathway
- Focal adhesion
- Insulin signaling pathway
- Melanoma
- Neurotrophin signaling pathway
- Non-small cell lung cancer
- Pancreatic cancer
- Pathways in cancer
- Prostate cancer
- VEGF signaling pathway
- Related Disease
- Cardiovascular Diseases
- Diabetes Mellitus, Type 2
- Edema
- Genetic Predisposition to Disease
- Lymphoma, Non-Hodgkin
- Parkinson Disease
- Parkinson disease
- Thyroid Neoplasms
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