
Human CD11b (alpha M integrin) complexes with CD18 (beta 2 integrin) to form the complement receptor type 3 (CR3) heterodimer which binds to ICAM-1, fibrinogen, C3b, and coagulation factor X. CD11b expression is restricted to leukocytes, mainly to myeloid and NK cells.
Isotype: Murine IgG1
Immunogen: Human monocytes
Specificity: Antibody ICRF44 recognizes the human integrin alpha M subunit (CD11b) of about 165 kd (1, 3). It has been reported to be cross reactive with porcine CD11b as well as cynomolgus monkey CD11b (6).
Functional Application: Antibody ICRF44 blocks homotypic neutrophil and monocyte (FMLP induced) aggregation.
References:
1. V. Mathotra, et al, (1986) Eur J Immunol 16: 1117-1123.
2. B.L. Myones, et al, (1988) J Clin Invest 82: 640-651.
3. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 555-558, 560-562.
4. H.R. Petty & R.F. Todd, (1993) J Leuk Biol 54: 492-494.
5. Leukocyte Typig V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995)p. 1588-1590.
6. Yoshino N, Honda M, et al. (2000) Exp Anim 49(2):97-110.
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第一代热启动Taq酶是一种DNA聚合酶。 第一代指的是直接从Taq(这是一种细菌)体内提取的天然聚合酶,没有进行化学修饰的,为了达到更好的聚合催化效果,往往需要经过酶工程处理,得到的聚合酶催化效率更高。 热启动是指这种酶的反应温度条件
Taq DNA polymerase是从克隆有Thermu aquaticus DNA Polymerase基因的大肠杆菌经诱导表达后,再经过柱纯化分离出来的一个约94 KD的重组蛋白。无外源核酸酶和细菌DNA污染,稳定性好,特异性强,适用于常规PCR扩增。

