
- Replacement tips for KWIK-TIP Ion Selective Electrodes
Replacement tips
Set of 3 hydrogen replacement tips for ion selective electrodes. Change the tip on your holder in less than a minute.
Notes
The tips should be stored dry under refrigeration prior to use. Once filled, the membrane tip can last up to 30 days and be used for that length of time. The tip is fragile and the “membrane” gets softer when wetted. After use, the tips can be placed into storage. Before storage, wash the exterior and carefully blot it dry. Leave the filling solution inside the electrode and seal it with parafilm. Then, place it in a dry container and store it in the refrigerator.
The KWIK Ag/AgCl pellet should be washed in distilled water, dried and stored in a dry, dark location. If desired, you may change to a new ionic solution, however, it takes time for the old ionic solution to exchange out of the KWIK pellet and be replaced with the new ionic concentration.This should be done in a small beaker and the contaminated solution should be discarded. The new tip and electrode should be filled with fresh solution.
Kwik-Tip Instruction Manual
Electrode | Color Code | Filling Solution | Nim. Slope/Decade | Concentration Range | Selectivity Coeffiecients (-log) | |
TIPCA | Calcium | Green | 0.1M CaCl2 | 28mV | 0.1 - 10-6.75M | Na+ 5.5, K+ 5.4, Mg++ 4.9 |
TIPH | Hydrogen | Orange | 1M Citric acid, 0.01M NaCl, pH 5.6 | 54mV | pH 5.0-12 | Na+ 10.4, K+ 9.8, Ca++ 11.1 |
TIPK | Potassium | Yellow | 0.1M KCl | 54mV | 0.1 - 10-4.5M | Na+ 4.0, Ca++ 3.9, Mg++3.0 |
TIPTPP | TPP+ | Purple | 10mM TPP+ | 54mV | 0.001 - 10-4M | K+ 6.0 |
- Dri-Ref Reference Electrode, 4.7 mm (short)DRIREF-5SHFor pricing, Customers outside of the US and Canada, please contact your distributor.
- Dri-Ref Reference Electrode, 4.7 mmDRIREF-5For pricing, Customers outside of the US and Canada, please contact your distributor.
- Dri-Ref Reference Electrode with Luer TipDRIREF-LFor pricing, Customers outside of the US and Canada, please contact your distributor.
- Electrode Holder for KWIK-TipsKWIK-2For pricing, Customers outside of the US and Canada, please contact your distributor.
- Dri-Ref Reference Electrode, 2 mmDRIREF-2For pricing, Customers outside of the US and Canada, please contact your distributor.
- Flexible Dri-Ref Reference ElectrodeFLEXREFFor pricing, Customers outside of the US and Canada, please contact your distributor.
- Micro Dri-Ref Reference ElectrodeDRIREF-450For pricing, Customers outside of the US and Canada, please contact your distributor.
- Dri-Ref Reference Electrode, ShortDRIREF-2SHFor pricing, Customers outside of the US and Canada, please contact your distributor.
- Potassium Electrode Tips, ReplacementTIPKFor pricing, Customers outside of the US and Canada, please contact your distributor.
- Calcium Electrode Tips, ReplacementTIPCAFor pricing, Customers outside of the US and Canada, please contact your distributor.
- TPP Electrode Tips, ReplacementTIPTPPFor pricing, Customers outside of the US and Canada, please contact your distributor.
- Super Dri-Ref Reference ElectrodeSDR2For pricing, Customers outside of the US and Canada, please contact your distributor.
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②成熟的tRNA分子中有许多的稀有碱基,因此tRNA在甲基转移酶催化下,某些嘌呤生成甲基嘌呤如A→mA,G→mA。有些尿嘧啶还原为双氢尿嘧啶。尿嘧啶核苷转变不假尿嘧啶核苷。某些腺苷酸脱氨基为成为次黄嘌呤核苷酸(Ⅰ)
③3’末端加上CCA:在核苷酸转移酶作用下,3’--末端除去个别碱基后,换上tRNA分子统一的CCA-OH末端,完成tRNA分子中的氨基酸臂结构。
siRNA:Small interfering RNA (siRNA):是一种小RNA分子(~21-25核苷酸),由Dicer(RNAase Ⅲ家族中对双链RNA具有特异性的酶)加工而成。SiRNA是siRISC的主要成员,激发与之互补的目标mRNA的沉默。
siRNA有如下特点:
1. 长度约在22nt左右。
2. 依赖Dicer酶的加工,是Dicer的产物,所以具有Dicer产物的特点。
3. 生成需要Argonaute家族蛋白存在。
4. 是RISC组分。
5. siRNA合成是由双链的RNA或RNA前体形成的。
6. siRNA一般是人工体外合成的,通过转染进入人体内,是RNA干涉的中间产物;植物体内也存在内源的siRNA。
7. 结构上, siRNA是双链RNA。
8. 在Dicer酶的加工过程中, siRNA对称地来源于双链RNA的前体的两侧臂。
9. 在作用位置上, siRNA可作用于mRNA的任何部位,并与mRNA完全互补。
10. 在作用方式上, siRNA只能导致靶标基因的降解,即为转录水平后调控。
11. siRNA不参与生物生长,是RNAi的产物,原始作用是抑制转座子活性和病毒感染。
补充,还有说常见的有丝氨酸水解酶家族,有文献提及赖氨酸。请问这些是如何选择的
二:主要功能:①运输功能②在逆转录作用中作为合成互补链DNA链的引物。③在细菌细胞壁、叶绿素、脂多糖和氨酰磷脂酰甘油的合成中都与某些tRNA的参与有关。
请问大神,siRNA一定是瞬转;如果瞬转,比如只能维持个2-3天,那么如果我想观察药物A作用于siRNA干预的细胞的效果,但是这个药物A需要作用10天,那么我间隔2-3天加一点SIRNA,这样可以吗,谢谢大神了
tRNA不是一条直的单链,而是弯曲的,呈现一个三叶草的形状。在弯曲的部位,tRNA自己的碱基跟自己的碱基互补配对连起来,碱基对中存在氢键。
其他的RNA一般为单链不存在氢链,但双链的由于碱基互补配对存在氢键。
与小分子siRNAs相比,尽管两者在分子特性、生物起源等方面是相似的,但也存在不少的差异.siRNAs是由dsDNA在Dicer酶切割下产生,而成熟miRNAs的产生要复杂一些,首先pri-miRNA在核内由一种称为Drosha酶处理后成为大约70nt的带有茎环结构的Precursor miRNAs (pre-miRNAs)(Denli et al.,2004; Gregory et al.,2004; Han et al.,2004);这些pre-miRNAs在Exportin-5帮助下转运到细胞核外之后再由胞质Dicer酶进行处理,酶切后成为成熟的miRNAs(Lund et al.,2004; Yi et al.,2003).两者的作用机制上也存在差别,成熟的miRNAs则是通过与miRNP核蛋白体复合物结合,识别靶mRNA,并与之发生部分互补,从而阻遏靶mRNA的翻译.在动物中,成熟的单链miRNAs与蛋白质复合物miRNP结合,引导这种复合物通过部分互补结合到mRNA的3′UTR(非编码区域),从而阻遏翻译.而在siRNA通路中,单链的siRNA结合到RISC复合物中,引导复合物与mRNA完全互补,通过其自身的解旋酶活性,解开siRNAs,通过反义siRNA链识别目的mRNA片段,通过内切酶活性切割目的片段,接着再通过细胞外切酶进一步降解目的片段.除此之外,miRNA也可以切割完全互补的mRNA,而siRNA也可以阻遏3′UTR具有短片断互补的mRNA的翻译.
siRNA (Small interfering RNA),是一种小RNA分子(~21-25核苷酸),由Dicer(RNAase Ⅲ家族中对双链RNA具有特异性的酶)加工而成。SiRNA是siRISC的主要成员,激发与之互补的目标mRNA的沉默。

