Hito Luxol Fast Blue - PAS OptimStain™ Kit is designed based on Luxol fast blue and Periodic acid-Schiff (PAS) method. This kit is made in a ready-to-use format and offers high quality, rapid staining (can be finished in one hour) of myelin/myelinated axons on paraffin sections or frozen sections. It has been proven to be extremely reliable and sensitive to demonstrate the morphological details of myelin fibers.Myelin is a substance rich in lipids and proteins that forms a layer, the myelin sheath, around only the axon of a neuron and acts as insulation. It is essential for the proper functioning of the nervous system. Demyelination is the loss of the myelin sheath insulating the nerves. This impairs the conduction of signals in the affected nerves, causing impairment in sensation, movement, and cognition. Currently, no cure exists for demyelinating diseases and myelin repair is an active research field. Hito Luxol Hito Luxol Fast Blue - PAS OptimStain™ Kit allows sensitive localization and visualization of the myelin fibers. PAS counterstain provides color contrasts which show morphological features that are particularly significant in the tissue, thus offers a fast and reliable way to determine the extent of demyelination.Hito Luxol Fast Blue - PAS OptimStain™ Kit has been tested extensively on the brains and spinal cords from several species of animals and it is a simple solution for your research.
| Kit Contents | Standard Kit | Small Kit |
| Solution-1 (LFB Solution 10xConcentrate) | 125 ml | 60 ml |
| Solution-2 (Li2CO3 Solution 5xConcentrate) | 250 ml | 120 ml |
| Solution-3 (Periodic Acid Solution) | 125 ml | 60 ml |
| Solution-4 (Modified Schiff"s Solution) | 125 ml | 60 ml |
| Solution-5 | 30 ml | 15 ml |
| Solution-6 | 125 ml | 60 ml |
| Dropper Bottle | 1 | 1 |
| Staining Jars | 6 | 6 |
| User Manual and MSDS | 1 | 1 |
Before using Hito Luxol Fast Blue - PAS OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit): Cryostat or Microtome and light microscopeParaffin embedding equipment (or paraffin sections)Hito Bouin’s Plus Solution (Cat# HTSH0104)Dry ice, isopentane, O.C.T. compound (for frozen sections), ethanol, xylene, 4% PFA (Hito Cat# HTSHS0102), double distilled or deionized waterSlides and coverslipsStaining jars for slides washResinous mounting medium  Hito Luxol Fast Blue - PAS OptimStain™ Kit Manual and MSDS | ||
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2、电泳问题：如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家：普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家：深圳匹基,上海之江,中山达安,上海科华,上海复星.
这个是用于代理授权的，这个可以说是唯一辨别产品真伪的方法，
有证书的企业，销售的产品可信度比较高。
有试剂A和B，传说10-30分钟就可以提取出来。不知道属不属实啊？
而且1000次580块钱！如果有用过的，可否跟我传授一下心得啊？
