
ProductHighlights:
TheTruSeqCustomAmpliconLowInputLibraryPrepKitisascalableampliconsequencingsolutionthatdeliverssensitiveandspecificresultsfrombothlow-inputandformalin-fixed,paraffin-embedded(FFPE)DNAsamples.Itoffers:
- Accuratevariantdetectionfromaslowas10ngofinputgenomicDNA(gDNA)andchallengingFFPEsamples
- Fullysupported,optimizedworkflowsolutionincludingsimpledataanalysis
- Automation-friendlyworkflowthatcanbecompletedin6hours,withonly3hoursofhands-ontime
LowDNAInputandFFPECompatibility
TheTruSeqCustomAmpliconLowInputLibraryPrepKitisafullycustomizable,amplicon-basedassayfortargetedresequencingstartingfromaslowas10ngofgenomicDNA(gDNA).Thisscalableassayallowsresearcherstocapturemultipletargetsofinterestsimultaneouslyandsequenceupto1536ampliconsinasinglepool,usingasinglereaction.ThislibraryprepkitofferstheflexibilitytoaccommodateFFPEsamples,suchaspreservedtumortissue.
ConfidentAssayDesign
DesigntargetedTruSeqCustomAmpliconoligonucleotideprobeswithDesignStudio,afree,easy-to-useonlinetoolthatprovidesoptimizedcoverage.
LearnmoreaboutDesignStudio
StartaprojectinDesignStudionow(loginrequired)
AssessFFPESampleQualitybeforeSequencing
TheTruSeqFFPEDNALibraryPrepQCKitusesasimpleqPCRreactiontodetermineDNAqualityandprovideguidanceonsequencingparameters.Thisstepensuresthatonlysamplesthatwillachievethenecessarysequencingmetricsareprepared,conservingresourcesthatmightbeusedonpotentiallylow-quality,unrecoverablesamples.TheresultsoftheQCstepdeterminetherecommendedamountofinputDNA.TheTruSeqFFPEDNALibraryPrepQCKitcanbebundledwiththeTruSeqCustomAmpliconLowInputLibraryPrepKittomaximizelabbudgets.
ForFFPEapplications,wesuggestdesigningyourprojectwith150bpor175bpampliconssinceFFPEDNAishighlydegraded.TruSeqCustomAmpliconLowInputcannotbeusedwithapplicationsrequiring425bpamplicons.
SpeciesCompatibility
TheTruSeqCustomAmpliconLowInputLibraryPrepKitsupportshuman,mouse,rat,andbovinesamples.DesignStudiodesignalgorithmsarealsoavailableformaize,rice,pig,dog,soybean,chicken,andsheep;however,assayperformancecannotbeguaranteedastheresultingassayshavenotbeentested.Inaddition,IlluminaConciergeservicescandesigncustomassaysand/orprovideassayoptimizationsupportforanyspeciesofinterest.
InquireaboutIlluminaConciergeservices
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
AssayTime | 1day |
Hands-OnTime | 4hours |
InputQuantity | 50ngRNA,50nghigh-qualitytotalRNA,≥200ngFFPEtotalRNA;Recommendedquantitymayvarywithexpressionlevel,targetplexity,andsamplequality |
ContentSpecifications | Choosefrom400,000+pre-designedtargetedRNA-Seqassays.Oraddcontenttoafixedpanelorpreviouslydesignedcustompanel. |
Multiplexing | Upto384samplespersequencingrun |
MechanismofAction | Amplification |
TruSeqCustomAmpliconLibraryPrepProtocol:

SeamlessAmpliconSequencingWorkflow:

RobustVariantDetectionatLowDNAInput:

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这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
有试剂A和B,传说10-30分钟就可以提取出来。不知道属不属实啊?
而且1000次580块钱!如果有用过的,可否跟我传授一下心得啊?
便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
普通的手提或者试剂盒提取的土壤基因组DNA由于常常残留有PCR的强烈抑制物如腐殖酸、棕黄酸等杂质造成实验失败,此外,由于采用了剧烈的玻璃珠击打来破裂菌体,常常造成DNA剪切和降解。本公司经过长期研发开发出了具有自主知识产权的土壤基因组DNA,通过专利配方的腐殖酸和棕黄酸去除试剂配合特殊处理的纯化柱,可以最大程度的去除这些杂质,同时加上多次柱漂洗,确保得到的DNA具有极高纯度,此外独特的抽提和裂解体系可以迅速裂解细胞(壁)和灭活细胞内核酸酶,不需要借助玻璃珠破壁,有效保证了基因组DNA的完整性。
产品特点:
1.本公司独有的专利配方和纯化柱能有效去除腐殖酸等杂质。
2.不需要借助玻璃珠破壁,有效保证了基因组DNA的完整性,长度可达30kb -50kb,可直接用于PCR,Southern-blot和各种酶切反应。
3.兼容性强,适用于各种不同的土壤包括淤泥等提取困难的土壤。
4.多步骤去除各种杂质和抑制物,保证了极高纯度,OD260/OD280典型的比值达1.7~1.9。
5.不需要使用有毒的苯酚等试剂,也不需要乙醇沉淀等步骤。
6.快速,简捷,单个样品操作一般可在60分钟内完成。
你找下,杭州昊鑫生物


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