To minimize sample-handling time, look no further than this real-time RT-PCR kit. PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior toreal-time RT-PCR (qPCR) with a streamlined workflow. cDNA synthesis reactions are easy to assemble—just add RNA template and water. The real-time RT-PCR kit contains a 5X master mix reagent that includes all reagents required for cDNA synthesis (PrimeScript RTase, RNase inhibitor, random 6mers, oligo dT primer, dNTPs, and reaction buffer). Because this real-time RT-PCR kit is powered by PrimeScript Reverse Transcriptase, it enables full-length cDNA synthesis for real-time RT-PCR (qPCR) in just 15 minutes. This kit is designed for two-step real-time RT-PCR. Use it upstream of a TB Green- or probe-based qPCR premix from Takara Bio, such as TB Green Premix Ex Taq II (Tli RNase H), TB Green Premix Ex Taq (Tli RNase H), or Premix Ex Taq (Probe qPCR).
To minimize sample-handling time, look no further than this real-time RT-PCR kit. PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior toreal-time RT-PCR (qPCR) with a streamlined workflow. cDNA synthesis reactions are easy to assemble—just add RNA template and water. The real-time RT-PCR kit contains a 5X master mix reagent that includes all reagents required for cDNA synthesis (PrimeScript RTase, RNase inhibitor, random 6mers, oligo dT primer, dNTPs, and reaction buffer). Because this real-time RT-PCR kit is powered by PrimeScript Reverse Transcriptase, it enables full-length cDNA synthesis for real-time RT-PCR (qPCR) in just 15 minutes. This kit is designed for two-step real-time RT-PCR. Use it upstream of a TB Green- or probe-based qPCR premix from Takara Bio, such as TB Green Premix Ex Taq II (Tli RNase H), TB Green Premix Ex Taq (Tli RNase H), or Premix Ex Taq (Probe qPCR).
This real-time RT-PCR kit includes PrimeScript reverse transcriptase, which has exceptionally strong strand-displacement activity for optimal efficiency during cDNA synthesis. The PrimeScript recombinant RTase enzyme is robust, versatile, and well-suited for applications requiring full-length cDNA synthesis. PrimeScript RTase enables reverse transcription of virtually any RNA template, including templates that are GC-rich and/or have high levels of secondary structure, making this real-time RT-PCR kit ideal for both routine applications and challenging reverse transcriptase reactions alike. PrimeScript RTase is a modified, recombinant MMLV (Moloney murine leukemia virus) enzyme and is verified to be RNase H-minus. PrimeScript reverse transcriptase is engineered to allow full-length cDNA synthesis reactions at lower temperatures (e.g., 42°C), decreasing the risk of RNA degradation that occurs during conventional reactions performed at higher temperatures.
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我分别用3ul、5ul、8ul都试过了，没有任何条带，用别人别的方法提好的DNA做，却能出现结果，不知道是自己提取的DNA浓度不够，还是根本没有提取出来。
不知道谁用过，或者有更好的全血提取NDA的试剂盒推荐呢？
本人刚刚开始做PCR，谢谢各位高手！
普通的手提或者试剂盒提取的土壤基因组DNA由于常常残留有PCR的强烈抑制物如腐殖酸、棕黄酸等杂质造成实验失败，此外，由于采用了剧烈的玻璃珠击打来破裂菌体，常常造成DNA剪切和降解。本公司经过长期研发开发出了具有自主知识产权的土壤基因组DNA，通过专利配方的腐殖酸和棕黄酸去除试剂配合特殊处理的纯化柱，可以最大程度的去除这些杂质，同时加上多次柱漂洗，确保得到的DNA具有极高纯度，此外独特的抽提和裂解体系可以迅速裂解细胞（壁）和灭活细胞内核酸酶，不需要借助玻璃珠破壁，有效保证了基因组DNA的完整性。
产品特点：
1.本公司独有的专利配方和纯化柱能有效去除腐殖酸等杂质。
2.不需要借助玻璃珠破壁，有效保证了基因组DNA的完整性，长度可达30kb -50kb，可直接用于PCR，Southern-blot和各种酶切反应。
3.兼容性强，适用于各种不同的土壤包括淤泥等提取困难的土壤。
4.多步骤去除各种杂质和抑制物，保证了极高纯度，OD260/OD280典型的比值达1.7～1.9。
5.不需要使用有毒的苯酚等试剂，也不需要乙醇沉淀等步骤。
6.快速，简捷，单个样品操作一般可在60分钟内完成。
你找下，杭州昊鑫生物
便宜点的就是国产的tiangen，效果会差一点点 如果是大样本的全血 用这个也凑合
这个是用于代理授权的，这个可以说是唯一辨别产品真伪的方法，
有证书的企业，销售的产品可信度比较高。
有试剂A和B，传说10-30分钟就可以提取出来。不知道属不属实啊？
而且1000次580块钱！如果有用过的，可否跟我传授一下心得啊？
