
EnzyChrom™ Creatinine Assay Kit
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Application
- Creatinine determination in urine, serum, plasma, and other biological preparations.
Key Features
- Fast and sensitive. Linear detection range: 10.6 to 500 µM or 0.12-5.7 mg/dL (colorimetric assay) and 0.25 to 50 µM or 0.0028-0.57 mg/dL (fluorimetric assay) for a 60 min reaction.
- Convenient. The procedure involves adding a single working reagent and reading after 60 minutes.
- High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day.
Method
- OD570nm; FL530/585nm
Samples
- Urine, serum, plasma, and other biological preparations.
Species
- All
Size
- 100 tests
Detection Limit
- Colorimetric assay: 10.6 µM or 0.12 mg/dL; Fluorimetric assay: 0.25 µM or 0.0028 mg/dL
Shelf Life
- 6 months
More Details
- CREATININE is synthesized in the body from creatine, which is produced during muscle contractions from creatine phosphate. In the blood, creatinine is removed by filtration through the glomeruli of the kidney and is secreted into urine. In healthy individuals, creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate. In kidney disease, creatinine levels in the blood are elevated, whereas the creatinine clearance rate and hence the urine levels are diminished. Creatinine testing is most widely used to assess kidney function.BioAssay Systems’ enzyme-based creatinine assay uses a reaction sequence that excludes both endogenous creatine and ammonia to convert a dye into a colored and fluorescent form. The absorbance at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the creatinine concentration in the sample.
What samples have you tested?
This kit has been tested and validated in urine, plasma, and serum samples.Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol.How do you use this kit on deproteinated plasma or serum samples with an internal standard procedure?
Samples will need three separate reactions: 1) sample plus standard2) sample alone 3) sample blank. For the internal standard prepare 200 µL 1250 µM standard by mixing 125 µL 2 mM Standard and 75 µL dH2O. For the sample plus standard well, add 5 µL 1250 µM standard and 20 µL sample. For the sample and sample blank wells, add 5 µL dH2O and 20 µL sample. Prepare enough Working Reagent (WR) for all assay wells by mixing, for each well, 80 µL Assay Buffer, 1 µL Enzyme A, 1 µL Enzyme Mix, 1 µL ATP, 1 µL Dye Reagent. Prepare a WR without the Enzyme A for the sample blank well(s). Add 80 µL of the WR to each well. Tap plate briefly to mix. Incubate at room temperature for 60 min. Use a plate reader to read OD570nm. The sample creatinine concentration is computed as follows: [Creatinine] = (RSAMPLE – RBLANK)/(RSTANDARD – RSAMPLE) x [Standard]/4 x n(µM)where RSAMPLE, RBLANK, and RSTANDARD are the OD or fluorescence values of the Sample, Sample Blank, and the Sample plus Standard respectively. n is the sample dilution factor. Note: The volume of the internal standard is 4× lower than the sample volume; thus, the internal standard concentration should be divided by 4.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.ebiomall.com






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Illuminamiseq测序是不是只测DNA的5到3方向的单链,而3到5在簇单链生成的时候被去掉了?是在rd1和index测序之前只有3到5单链共价结合在flowcell上?
DNA测序是指分析特定DNA片段的碱基序列,也就是腺嘌呤(A)、胸腺嘧啶(T)、胞嘧啶(C)与鸟嘌呤的(G)排列方式。快速的DNA测序方法的出现极大地推动了生物学和医学的研究和发现。
其原理是化学试剂处理末段DNA片段,造成碱基的特异性切割,产生一组具有各种不同长度的DNA链的反应混合物,经凝胶电泳分离。化学切割反应:包括碱基的修饰修饰的碱基从其糖环上转移出去在失去碱基的糖环处DNA断裂。
另一个原理是利用一种DNA聚合酶来延伸结合在待定序列模板上的引物。直到掺入一种链终止核苷酸为止。每一次序列测定由一套四个单独的反应构成,每个反应含有所有四种脱氧核苷酸三磷酸(dNTP),并混入限量的一种不同的双脱氧核苷三磷酸(ddNTP)。由于ddNTP缺乏延伸所需要的3-OH基团,使延长的寡聚核苷酸选择性地在G、A、T或C处终止。终止点由反应中相应的双脱氧而定。每一种dNTPs和ddNTPs的相对浓度可以调整,使反应得到一组长几百至几千碱基的链终止产物。它们具有共同的起始点,但终止在不同的的核苷酸上,可通过高分辨率变性凝胶电泳分离大小不同的片段,凝胶处理后可用X-光胶片放射自显影或非同位素标记进行检测。
正向测不通,可能是因为:
1、你的模板比较困难,比如GC含量局部比较高,形成发夹结构,或出现连续的G、C,也可能是连续的C、T。而生工一板要做384或96个反应,用的PCR条件是统一的,不可能兼顾到你的序列的特殊情况,因此你的序列PCR不出来
2、PCR纯化试剂盒有问题,可以考虑换纯化试剂盒。
3、测序时进样太多或太少
解决方法:
1、你可以试试用载体上的通用引物进行测序
2、已经设计出来的序列上设计一段引物,进行walking
2016年02月01日发布
关于公布注册检验用体外诊断试剂国家标准品和参考品目录的通知
为配合《体外诊断试剂注册管理办法》(国家食品药品监督管理总局令第5号)的实施执行,现将供注册检验用体外诊断试剂国家标准品和参考品形成目录,公布如下。
注册检验用体外诊断试剂国家标准品和参考品目录(第一期)
序号名称品种编号供应情况1促甲状腺素(TSH)免疫测定用国家标准品150530正常供应2促黄体生成素(LH)免疫测定用国家标准品1505313人绒毛膜促性腺激素β亚单位(hCG-β)免疫测定用国家标准品1505354人胎盘泌乳素(HPL)免疫测定用国家标准品1505365甲胎蛋白(AFP)免疫测定用国家标准物质1505426前列腺特异性抗原(PSA)免疫测定用国家标准物质1505437游离前列腺特异性抗原(f-PSA)免疫测定用国家标准品1505448三碘甲腺原氨酸(T3)免疫测定用国家标准品1505509甲状腺素(T4)免疫测定用国家标准品15055110反三碘甲腺原氨酸(rT3)免疫测定用国家标准品15055211胰高血糖素(Glucagon)免疫测定用国家标准品15055412人绒毛膜促性腺激素(HCG)免疫测定用国家标准品15055513淋病PCR试剂盒质控参考品21001514结核分支杆菌PCR检测试剂盒用国家参考品23003015结核分支杆菌利福平耐药基因检测试剂用国家参考品23003316结核分枝杆菌异烟肼耐药基因检测试剂用国家参考品23003417测序仪性能评价用脱氧核糖核酸国家参考品36000718人脲原体核酸检测国家参考品36000919第一代H7N9禽流感病毒核酸参考品37000120EB病毒衣壳抗原IgA抗体国家参考品37000221甲型流感病毒抗原检测试剂国家参考品37000322乙型流感病毒抗原检测试剂国家参考品37000423甲/乙型流感病毒核酸检测试剂国家参考品37000624扎伊尔型埃博拉病毒核酸检测试剂国家参考品37001025甲型流感病毒核酸检测试剂国家参考品37001126乙型流感病毒核酸检测试剂国家参考品37001227甲/乙型流感病毒抗原检测试剂国家参考品37001328甲型H1N1流感病毒核酸检测试剂国家参考品37001429抗HTLV抗体国家参考品220003限制供应,每企业只供应2套/年30HIV抗体国家参考品22000931SARS病毒抗体IgM国家参考品22001032SARS病毒抗体(总抗体或IgG)国家参考品22001133HIV-1RNA国家参考品22001734HIV-1耐药性分析试剂国家参考品22001835HIV抗体尿液快速检测试剂国家参考品22002036乙型肝炎病毒表面抗原(HBsAg)国家参考品30000337乙型肝炎病毒e抗原(HBeAg)国家参考品30000538乙型肝炎病毒e抗体(HBeAb)国家参考品30000639乙型肝炎病毒核心抗体(Anti-HBcAb)国家参考品30000740戊型肝炎病毒IgM抗体国家参考品30001141戊型肝炎病毒IgG抗体国家参考品30001442丙型肝炎病毒抗体国家参考品300010限制供应,每企业只供应1套/年
注:1.相关品种电子版说明书可到中国食品药品检定研究院网站http://www.nifdc.org.cn/bzwz/CL0481/,点击目录查询,进行在线浏览。
2.限制供应是指仅供给相关体外诊断试剂生产企业。
二〇一六年二月一日
1,测序技术的改进:全基因组主要应用的是illumina的Hiseq X10的测序平台,该平台在图片信息处理和flowcell上都有很大的改进(原来是平板上长簇,现在上面有小孔),另外在扩增的技术上也有一点改进(RPA扩增技术)。以上几点使得数据的通量大大调高。所以相应的测序价格也会降低。
2,市场占有策略:这一点我个人理解比技术更新更重要,illumina的战略思路,占有测序市场。其实illumina的Hiseq X10的测序平台试剂要比其他平台的试剂便宜很多。不过这个平台签有协议,只能做人的全基因组重测序项目。

