+,PEL-AMPKA-S487-T-1, PEL-AMPKA-S487-T-2, PEL-AMPKA-S487-T-5, Q13131, Q5EG47, P54645, PRKAA1, AMPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK subunit alpha-1) (EC 2.7.11.1) (Acetyl-CoA carboxylase kinase) (ACACA kinase) (EC 2.7.1蚂蚁淘商城

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RayBiotech/Human, Mouse and Rat Phospho-AMPKa1 (S487) and Total AMPKa1 ELISA/1 Plate Kit/PEL-AMPKA-S487-T-1

品牌 /

RayBiotech

货号 /

PEL-AMPKA-S487-T-1

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4000-520-616

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Antigen Information

Accession Number:

P54645
Q13131
Q5EG47

Gene ID:

5562

Gene Symbols:

AMPK1
PRKAA1

Protein Name & Synonyms:

5"-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK subunit alpha-1) (EC 2.7.11.1) (Acetyl-CoA carboxylase kinase) (ACACA kinase) (EC 2.7.11.27) (Hydroxymethylglutaryl-CoA reductase kinase) (HMGCR kinase) (EC 2.7.11.31) (Tau-protein kinase PRKAA1) (EC 2.7.11.26)

Target Species:

Human
Mouse
Rat

Assay Format

Pathway:

AMPK Signaling
Autophagy
Insulin Signaling

Specificity:

This ELISA kit recognizes Human

Number of Targets Detected:

Species Detected:

Human
Mouse
Rat

Compatible Sample Types:

Cell Lysates
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Colorimetric

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

96-well Microplate

Product Specifications

Size:

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Rapidly measure phosphorylated protein in lysates
Screen numerous different cell lysates without performing a Western Blot analysis
Minimal hands-on time, convenient, and non-radioactive material

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Anti-Phospho Antibody
HRP-Conjugated Secondary Antibody
Assay Diluent
TMB One-Step Substrate
Stop Solution
Lysis Buffer
Positive Control Sample

Other Materials Required

Distilled or deionized water
100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Protocol Outline

Prepare all reagents and samples as instructed in the manual.
Add 100 µl of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 ^°C.
Add 100 µl of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Typical Data

Positive Control

HeLa cells were treated with Calyculin A. Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.

Calyculin A Stimulation of HeLa Cell Lines

HeLa cells were treated or untreated with Calyculin A. Cell lysates were analyzed using this phosphoELISA and Western Blot.

Storage/Stability

Upon receipt, the kit should be stored at â20^°C. Please use within 6 months from the date of shipment.

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Manual

SDS

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