Antigen Information
- CSF2
- CXCL8
- IFNG
- IL10
- IL12A
- IL1B
- IL4
- IL6
- TGFB1
- TNF
Assay Format
- JAK/STAT Signaling
- MAPK Signaling
- NFkB Signaling
- Porcine (pig)
- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
- Sandwich-based
- Quantitative
Product Specifications
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
Scroll over each target protein for more information | ||||
---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 beta(IL-1 F2)
| IL-10
| IL-12 p70
|
IL-4
| IL-6
| IL-8(CXCL8)
| TGF beta 1
| TNF alpha
|
Application Notes
- Porcine Cytokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
- Sohn EH., Jiao C., Kaalberg E., et al. Allogenic iPSC-derived RPE cell transplants induce immune response in pigs: a pilot study. Sci Rep. 2015 Jul 3;5:11791. doi: 10.1038/srep11791.Species: PorcineSample type: Vitreous Fluid
- Goetz J., Fredericks D., Petersen E., et al. A clinically realistic large animal model of intra-articular fracture that progresses to post-traumatic osteoarthritis. Osteoarthritis Cartilage. 2015 Oct;23(10):1797-805. doi: 10.1016/j.joca.2015.05.022Species: PorcineSample type: Synovial Fluid
- Beltran-Beck B., de la Fuente J., Garrido J., Aranaz A., Sevilla I., et al. Oral Vaccination with Heat Inactivated Mycobacterium bovis Activates the Complement System to Protect against Tuberculosis. PLOS One, 2014 May 19, DOI: 10.1371/journal.pone.0098048Species: PorcineSample type: Serum
- Beltran-Beck B., Romero B., Boadella M., et al. Tonsils of the Soft Palate Do Not Mediate the Response of Pigs to Oral Vaccination with Heat-Inactivated Mycobacterium bovis. Clin Vaccine Immunol. 2014 Aug;21(8):1128-36. doi: 10.1128/CVI.00221-14Species: PorcineSample type: Serum
- Butz D., et al. The expired breath carbon delta value is a marker for the onset of sepsis in a swine model. Journal of Analytical Atomic Spectrometry. 2014, 29, 606-613. DOI: 10.1039/C3JA50340BSpecies: PorcineSample type: Plasma
- Pazos, M. R., Mohammed, N., Lafuente, H., Santos, M., Martínez-Pinilla, E., Moreno, E., ... & Hillard, C. J. (2013). Mechanisms of cannabidiol neuroprotection in hypoxic?ischemic newborn pigs: Role of 5HT 1A and CB2 receptors.?Neuropharmacology,?71, 282-291.Species: PorcineSample type: Tissue Lysate
- Mulloy DP, Stone ML, Crosby IK, et al.?Ex Vivo?Rehabilitation of Non-Heart-Beating Donor Lungs in a Preclinical Porcine Model: Delayed Perfusion Results in Superior Lung Function.?The Journal of thoracic and cardiovascular surgery. 2012;144(5):1208-1216. doi:10.1016/j.jtcvs.2012.07.056.Species: PorcineSample type: BAL Fluid
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要做细胞缺氧,需要买细胞缺氧盒,但不知道哪里有卖,各位大神有没有曾经做过的,能不能告诉我在哪能购买到,哪个公司有卖。。急。。。。。。实验小白。。。。在下先在此谢过了。
小弟现有一事儿想求助!
如下:1)凋亡的时候细胞色素c由线粒体里释放到胞浆里去的。那么如果能够测到此时在胞浆里的细胞色素c表达增多的话应该就能说明问题了
(2)可此时的问题是如果提胞浆蛋白时把线粒体也裂解了话细胞色素c也会被释放出来,还是会在胞浆里大量表达!就说不清楚是凋亡由线粒体释放的还是被人为的破坏了线粒体导致了的!!!
(3)故我的问题就是该怎么样去除这个人为的影响?怎么说明我测的胞浆里的细胞色素c就是凋亡时线粒体释放的?换句话讲就是如果能够在用细胞裂解液裂解胞浆蛋白时,将线粒体剔除掉?
是不是买个细胞色素c的测试盒就可以了啊?这个盒子有人用过吗,请指教一二!
盼复!非常感谢!
心肌肌钙蛋白I,英文为CardiactroponinI,该试剂盒在POCT领域内几乎是每个厂家都必做的项目,也是多数医院科室都开展的收费项目,市场容量很大,全国一年下来保守估计有3千万人份。但有些厂家销售的特别好,有些厂家销售的很一般。这一是由于销售渠道所致、二是因为产品质量差导致。试剂盒质量差一是因为厂家自身调试工艺不太好,二是由于所选择的抗体质量不好所致。现在市面上有心肌肌钙蛋白I抗体的厂家不多,大概也就不超过10家吧,主要为进口品牌,如:HyTest、BBI、Biospacific、Medix,国产品牌为深圳菲鹏,杭州启泰。其中着重要说的是杭州启泰,这是一家成立7年的抗原、抗体研发公司,公司不大,但立足于自主研发、创新,最近新推出的cTnI抗体可以说是目前国内外最好的抗体,这也算是填补了国内该领域的空白。从事了该行业将近10年,发此贴也是真切希望国内的生物试剂类公司,都专注于研发、创新,做一些好的试剂出来。因为很多事情国内做的并不比国外差的,国内的生物同行请加油吧!
以前先放-80再液氮,但是去-80冰箱有点远,我想能不能直接放液氮里面,我直接放过复苏成活率低了很多,有没有这种冻存盒可以直接放液氮的?