- GDC-0152
- Embelin
- Birinapant (TL32711)
- UC 112
- BV6
| LCL161Antagonist of IAPs inhibitor |
Sample solution is provided at 25 µL, 10mM.
Quality Control & MSDS
- View current batch:
- Purity = 99.81%
- COA (Certificate Of Analysis)
- HPLC
- MS (Mass Spectrometry)
- NMR (Mass Spectrometry)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
Related Biological Data
| Cell experiment [1]: | |
Cell lines | Hep3B, PLC5, Sk-Hep1 and Huh-7 cells |
Preparation method | The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reaction Conditions | 0, 0.01, 0.05, 0.1, 0.5, 1, 5 and 10 μM; 24, 48 or 72 hrs |
Applications | LCL161 showed significant inhibition of cell proliferation and viability in 2 human hepatocellular carcinoma (HCC) cells, Hep3B and PLC5. The IC50 values were 10 and 19 μM, respectively. However, LCL161 had no effect in 2 other HCC cell lines, Sk-Hep1 (IC50 value of 224 μM) and Huh-7 (IC50 value of 228 μM). |
| Animal experiment [1]: | |
Animal models | Huh-7 xeonograft nude mice |
Dosage form | 50 mg/kg; p.o.; q.d., for 20 days |
Applications | Co-treatment with LCL161 and SC-2001 showed significant anti-tumor effect on Huh-7 tumors, without affecting body weight significantly. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1]. Chen K F, Lin J P, Shiau C W, et al. Inhibition of Bcl-2 improves effect of LCL161, a SMAC mimetic, in hepatocellular carcinoma cells. Biochemical pharmacology, 2012, 84(3): 268-277. | |
LCL161 Dilution Calculator
calculate
LCL161 Molarity Calculator
calculate
| Cas No. | 1005342-46-0 | SDF | Download SDF |
| Synonyms | LCL-161;LCL 161 | ||
| Chemical Name | (2S)-N-[(1S)-1-cyclohexyl-2-[(2S)-2-[4-(4-fluorobenzoyl)-1,3-thiazol-2-yl]pyrrolidin-1-yl]-2-oxoethyl]-2-(methylamino)propanamide | ||
| Canonical SMILES | CC(C(=O)NC(C1CCCCC1)C(=O)N2CCCC2C3=NC(=CS3)C(=O)C4=CC=C(C=C4)F)NC | ||
| Formula | C26H33FN4O3S | M.Wt | 500.63 |
| Solubility | ≥25.05 mg/mL in DMSO, <2.58 mg/ml="" in="" etoh,=""><2.45 mg/ml="" in="" h2o=""> | Storage | Store at -20°C |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
LCL161 is a small molecular antagonist of the inhibitor of apoptosis (IAP) with IC50 value of 10.23 μM in Hep3B cells [1].IAP is a family of proteins which are firstly found in virus and to inhibit the apoptosis of the infected hosts. It contains eight proteins in human. Since they were always found to overexpress in variety of malignant tumors, the IAP are thought to be appropriate targets in cancer therapy. SMAC (second mitochondria-derived activator of caspases) is the first-identified antagonist of IAP. It binds to XIAP within the BIR2/3 domain through its N-terminal segment. As a mimetic of SMAC, LCL161 is designed to be the inhibitor of both XIAP and cIAP1/2 [1].LCL161 showed significant inhibition of cell proliferation and viability in two human hepatocellular carcinoma (HCC) cells, Hep3B and PLC5. The IC50 values were 10 and 19 μM, respectively. However, LCL161 had no effect in the two other HCC cell lines, Sk-Hep1 (IC50 value of 224 μM) and Huh-7 (IC50 value of 228 μM). The difference of the effects is found to dependent on the expression of Bcl-2 in cells. For the ALL cells, LCL161 exerted growth inhibition with IC50 values of 9.3 and 0.25 μM, respectively. LCL161 also showed effect on the ALCL cell line Karpas-299 with IC50 value of 1.6 μM [1, 2].In vivo, LCL161 markedly affected the distribution of EFS in many solid tumor xenograft models. It also caused growth delay in some tumors such as osteosarcoma, neuroblastoma and glioblastoma at dose of 30 mg/kg orally. Besides that, LCL161 administration caused significant growth inhibition in EW-5 and BT-39 glioblastoma but not in BT-28. Moreover, the combination therapy of LCL161 and the adeno-associated virus bacteriophage-tumor necrosis factor-α (AAVP-TNF-α) has been reported to has synergistic anti-tumor effects and delayed treatment resistance in mice models of tumor xenografts [1, 2 and 3].References:1.Chen K F, Lin J P, Shiau C W, et al. Inhibition of Bcl-2 improves effect of LCL161, a SMAC mimetic, in hepatocellular carcinoma cells. Biochemical pharmacology, 2012, 84(3): 268-277.2.Houghton P J, Kang M H, Reynolds C P, et al. Initial testing (stage 1) of LCL161, a SMAC mimetic, by the Pediatric Preclinical Testing Program. Pediatric blood & cancer, 2012, 58(4): 636-639.3.Yuan Z, Syrkin G, Adem A, et al. Blockade of inhibitors of apoptosis (IAPs) in combination with tumor-targeted delivery of tumor necrosis factor-α leads to synergistic antitumor activity. Cancer gene therapy, 2012, 20(1): 46-56.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
心肌肌钙蛋白I，英文为CardiactroponinI，该试剂盒在POCT领域内几乎是每个厂家都必做的项目，也是多数医院科室都开展的收费项目，市场容量很大，全国一年下来保守估计有3千万人份。但有些厂家销售的特别好，有些厂家销售的很一般。这一是由于销售渠道所致、二是因为产品质量差导致。试剂盒质量差一是因为厂家自身调试工艺不太好，二是由于所选择的抗体质量不好所致。现在市面上有心肌肌钙蛋白I抗体的厂家不多，大概也就不超过10家吧，主要为进口品牌，如：HyTest、BBI、Biospacific、Medix，国产品牌为深圳菲鹏，杭州启泰。其中着重要说的是杭州启泰，这是一家成立7年的抗原、抗体研发公司，公司不大，但立足于自主研发、创新，最近新推出的cTnI抗体可以说是目前国内外最好的抗体，这也算是填补了国内该领域的空白。从事了该行业将近10年，发此贴也是真切希望国内的生物试剂类公司，都专注于研发、创新，做一些好的试剂出来。因为很多事情国内做的并不比国外差的，国内的生物同行请加油吧!
小弟现有一事儿想求助！
如下：1）凋亡的时候细胞色素c由线粒体里释放到胞浆里去的。那么如果能够测到此时在胞浆里的细胞色素c表达增多的话应该就能说明问题了
（2）可此时的问题是如果提胞浆蛋白时把线粒体也裂解了话细胞色素c也会被释放出来，还是会在胞浆里大量表达！就说不清楚是凋亡由线粒体释放的还是被人为的破坏了线粒体导致了的！！！
（3）故我的问题就是该怎么样去除这个人为的影响？怎么说明我测的胞浆里的细胞色素c就是凋亡时线粒体释放的？换句话讲就是如果能够在用细胞裂解液裂解胞浆蛋白时，将线粒体剔除掉？
是不是买个细胞色素c的测试盒就可以了啊？这个盒子有人用过吗，请指教一二！
盼复！非常感谢！
要做细胞缺氧，需要买细胞缺氧盒，但不知道哪里有卖，各位大神有没有曾经做过的，能不能告诉我在哪能购买到，哪个公司有卖。。急。。。。。。实验小白。。。。在下先在此谢过了。
以前先放-80再液氮，但是去-80冰箱有点远，我想能不能直接放液氮里面，我直接放过复苏成活率低了很多，有没有这种冻存盒可以直接放液氮的？
