![Abnova/mutaFISH™ BRAF V600E V600wt RNA Probes/1kit/FP0011](images/no_picture.gif)
- Specification
- Product Description:
- mutaFISH™ BRAF V600E V600wt RNA Probes is designed to detect human BRAF V600E gene mutation on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
- Supplied Product:
- Content:1. RT BRAF V600 Primer2. mutaFISH™ BRAF V600E RNA Probe3. mutaFISH™ BRAF V600wt RNA Probe4. Detection Probe-Aqua 4315. Detection Probe-Texas Red X
- Storage Instruction:
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
- Note:
- We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
- Protocol:
Protocol Download
- Technology:
- mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
- Comparison:
- FISH Probes vs mutaFISH™ Probes
- Fluorophore:
- Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)Texas Red X (Excitation Peak (nm): 595; Emission Peak613)
- Probe Position:
- Regulatory Status:
- For research use only (RUO)
- Video:
- Datasheet:
Download
- Applications
- mutation specific, Fluorescence In Situ Hybridization (Cells)
- mutaFISH™ staining was performed in situ in human Sk-HEP1 cells. BRAF V600E point mutation was detected via orange signal (Cy3), BRAF V600wt was detected via red signal (Cy5).
- mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
- mutaFISH™ staining was performed in situ in mouse FFPE BRAF 293T tissue. BRAF V600wt was detected via red signal (Texas Red X).
- Application Image
- mutation specific, Fluorescence In Situ Hybridization (Cells)
- enlarge
- mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
- enlarge
- Gene Information
- Entrez GeneID:
- 673
- Gene Name:
- BRAF
- Gene Alias:
- B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1
- Gene Description:
- v-raf murine sarcoma viral oncogene homolog B1
- Omim ID:
- 115150, 164757, 211980
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
- Other Designations:
- 94 kDa B-raf protein,B-Raf proto-oncogene serine/threonine-protein kinase (p94),Murine sarcoma viral (v-raf) oncogene homolog B1
- Gene Pathway
- Acute myeloid leukemia
- Bladder cancer
- Chemokine signaling pathway
- Chronic myeloid leukemia
- Colorectal cancer
- Endometrial cancer
- ErbB signaling pathway
- Focal adhesion
- Glioma
- Insulin signaling pathway
- Long-term depression
- Long-term potentiation
- MAPK signaling pathway
- Melanoma
- mTOR signaling pathway
- Natural killer cell mediated cytotoxicity
- Neurotrophin signaling pathway
- Non-small cell lung cancer
- Pancreatic cancer
- Pathways in cancer
- Prostate cancer
- Regulation of actin cytoskeleton
- Renal cell carcinoma
- Thyroid cancer
- Vascular smooth muscle contraction
- Related Disease
- Abnormalities, Multiple
- Adenocarcinoma
- Adenocarcinoma, Follicular
- Adenocarcinoma, Mucinous
- Adenocarcinoma, Papillary
- Adenoma
- Adenoma, Oxyphilic
- Adrenal Cortex Neoplasms
- Alcoholism
- Articulation Disorders
- Astrocytoma
- Brain Neoplasms
- Breast cancer
- Breast Neoplasms
- Carcinoma
- Carcinoma, Basal Cell
- Carcinoma, Medullary
- Carcinoma, Non-Small-Cell Lung
- Carcinoma, Papillary
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心肌肌钙蛋白I,英文为CardiactroponinI,该试剂盒在POCT领域内几乎是每个厂家都必做的项目,也是多数医院科室都开展的收费项目,市场容量很大,全国一年下来保守估计有3千万人份。但有些厂家销售的特别好,有些厂家销售的很一般。这一是由于销售渠道所致、二是因为产品质量差导致。试剂盒质量差一是因为厂家自身调试工艺不太好,二是由于所选择的抗体质量不好所致。现在市面上有心肌肌钙蛋白I抗体的厂家不多,大概也就不超过10家吧,主要为进口品牌,如:HyTest、BBI、Biospacific、Medix,国产品牌为深圳菲鹏,杭州启泰。其中着重要说的是杭州启泰,这是一家成立7年的抗原、抗体研发公司,公司不大,但立足于自主研发、创新,最近新推出的cTnI抗体可以说是目前国内外最好的抗体,这也算是填补了国内该领域的空白。从事了该行业将近10年,发此贴也是真切希望国内的生物试剂类公司,都专注于研发、创新,做一些好的试剂出来。因为很多事情国内做的并不比国外差的,国内的生物同行请加油吧!
以前先放-80再液氮,但是去-80冰箱有点远,我想能不能直接放液氮里面,我直接放过复苏成活率低了很多,有没有这种冻存盒可以直接放液氮的?
小弟现有一事儿想求助!
如下:1)凋亡的时候细胞色素c由线粒体里释放到胞浆里去的。那么如果能够测到此时在胞浆里的细胞色素c表达增多的话应该就能说明问题了
(2)可此时的问题是如果提胞浆蛋白时把线粒体也裂解了话细胞色素c也会被释放出来,还是会在胞浆里大量表达!就说不清楚是凋亡由线粒体释放的还是被人为的破坏了线粒体导致了的!!!
(3)故我的问题就是该怎么样去除这个人为的影响?怎么说明我测的胞浆里的细胞色素c就是凋亡时线粒体释放的?换句话讲就是如果能够在用细胞裂解液裂解胞浆蛋白时,将线粒体剔除掉?
是不是买个细胞色素c的测试盒就可以了啊?这个盒子有人用过吗,请指教一二!
盼复!非常感谢!
要做细胞缺氧,需要买细胞缺氧盒,但不知道哪里有卖,各位大神有没有曾经做过的,能不能告诉我在哪能购买到,哪个公司有卖。。急。。。。。。实验小白。。。。在下先在此谢过了。
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