Recombinant Human FAM105B/OTULIN Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-558
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: FAM105B/OTULIN
FAM105B ("OTULIN") is a C65 type peptidase and a member of the ovarian tumor (OTU) protein super-family. OTULIN has exquisite specificity for linear (methionine-1-linked) ubiquitin linkages and has been shown to play a role in the regulation of NF-kB mediated signaling.
- Keusekotten, K. et al. (2013) Cell 153: 1312.
- Mevissen, T.E. et al. (2013) Cell 154: 169.
- Radjacommare, R. et al. (2014) Front Plant Sci. 5: 84.
Citation for Recombinant Human FAM105B/OTULIN Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- OTULIN limits cell death and inflammation by deubiquitinating LUBACAuthors: K Heger, KE Wickliffe, A Ndoja, J Zhang, A Murthy, DL Dugger, A Maltzman, F de Sousa E, J Hung, Y Zeng, E Verschuere, DS Kirkpatric, D Vucic, WP Lee, M Roose-Girm, RJ Newman, S Warming, YC Hsiao, LG K?m?ves, JD Webster, K Newton, VM DixitNature, 2018;559(7712):120-124.Species: MouseSample Types: Cell LysatesApplications: Bioassay
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