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- Specifications
Additional Information
Catalog #32-5106SpeciesHumanRegulatory StatusResearch Use Only. Not for Use in Diagnostic Procedures.Product DistributionAvailable in North America OnlyRange1.95 - 250 pg/mLSensitivity0.8 pg/mLSizes96 WellsSample TypesPlasmaIncubation Time3 hrSample Size100µLInstructions for UseClick here to viewSafety Data SheetClick here to view - Additional Information
- Additional Information
Ghrelin is a 3.5 kDa protein shown to be involved in the regulation of many physiological processes including memory retention, sleep, insulin secretion, food intake, fat utilization, and release of cytokines.
Ghrelin is present in the peripheral circulation in two forms: acylated and non-acylated. The Acylated Ghrelin ELISA specifically measures the acylated form of ghrelin. The Acylated Ghrelin ELISA is based on a double-antibody sandwich technique; the Wells of the plate supplied with the kit are coated with a monoclonal antibody specific to the C-terminal part of ghrelin. This antibody will bind to any ghrelin introduced into the wells (standard or sample). The AChE - Fab` conjugate which recognizes the N-terminal part of acylated ghrelin is also added to the wells. This allows the two antibodies to form a sandwich by binding on different parts of the human acylated ghrelin. Each kit contains ghrelin (acylated) AChE-Fab` conjugate, human acylated ghrelin standard, human acylated ghrelin quality control, Ellman’s reagent, EIA buffer, wash buffer, Tween 20, plates pre-coated with anti-ghrelin mouse monoclonal antibody, and complete instructions.
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先谢了
数据分析的小白一枚，最近开始学习二代测序仪IONTORRENT出来的靶向基因检测位点的数据分析。由于完全没有涉猎过这个领域，会有很多不懂得地方，望大神们不吝赐教！
比如上面这个搜索结果，就是根据一起出来的数据，也就是一个位点的rs号进行检索出来的界面，但是完全不知道如何下手，也不是很清楚具体模块的作用，我能得到什么结果。虽然在网上能看到一些说明，但是仍然一知半解的！
最后，还是想说，大神们快快带我入门吧，非常迫切！跪谢！
在非变性聚丙烯酰胺凝胶电泳时，它们的迁移率各不相同，从而获得单倍型特异的电泳带格局即PCR指纹。也有人用人工合成的短寡核苷酸片段作为探针，同经过酶切的人体DNA作Southern blot，可以得出长度不等的杂交带，杂交带的数目和分子量的大小具有个体特异性，除非同卵双生，几乎没有两个人是完全相同的，就象人的指纹一样，人们把这种杂交带图形称为基因指纹(gene finger-printing)。
基因芯片法：又称为DNA 微探针阵列（Micro array）。它是集成了大量的密集排列的大量已知的序列探针，通过与被标记的若干靶核酸序列互补匹配，与芯片特定位点上的探针杂交，利用基因芯片杂交图象，确定杂交探针的位置，便可根据碱基互补匹配的原理确定靶基因的序列。这一技术已用于基因多态性的检测。对多态性和突变检测型基因芯片采用多色荧光探针杂交技术可以大大提高芯片的准确性、定量及检测范围。应用高密度基因芯片检测单碱基多态性，为分析SNPs提供了便捷的方法。
亲子鉴定？
ID,promega.我们实验室主要用的是这两种，由于怕有突变还会备有凯杰的。
如果测序的话，那么就是AB的bigdye吧？
有什么问题我们可以交流。
