Designed for eye research
- Includes SilFlex tubing, gasket, holder and blunt tip assortment
- NanoFil syringe sold separately
- Less than 3 μL dead volume
- Achieve accurate, repetitive, oil-free injections
- Application example: pump, stand probe holder and micropositioner not included
Options
| Order code | Description |
| RPE-KIT | Blunt tip |
| IO-KIT | Beveled tip |
Click here to view the current NanoFil Data Sheet
Benefits
- No oil back filling necessary
- Comes with four needle sizes included in the kit
Applications
- Retinal pigment epithelium injection
- Mouse brain injection
- Intravitreal Injection (with RPE-KIT)
- Intraoccular Injection (with IO-KIT)
These kits are specially designed for eye research for injecting retinal pigment epithelium (RPE) and intraocular (IO)in addition to brain injection in mice. They are used exclusively with a a NanoFil™ syringe and UMP3to achieve accurate and repeatable oil free injections down to submicroliter ranges. Each kit includes two pieces ofSilflextubing, a holder assembly, spare gaskets, and an assortment of four needles — blunt for the RPE kit and beveled tips for the IO kit. Each kit comes with one each of 33, 34, 35 and 36 gauge needles so that first time users can find the best size for their application.
The Silflex tubing is a very important component of the kit. This 35cm long, flexible tubing has a precise outer diameter for airtight fitting with the syringe. It also has a small inner diameter to minimize dead volume. The SilFlex is coupled to the injection tip with a seal system similar to that of the NanoFil. The dead volume of the entire kit (including the tubing) is less than 3microliters. All of the components in the kit are constructed of inert, solvent resistant materials for easy cleaning after viral injection.
Includes:
The kit includes two pieces of Silflex tubing, a holder assembly, spare gaskets, and one each of the blunt needles: 33g, 34g, 35g and 36g.
Park, S. W., Kim, J. H., Park, W. J., & Kim, J. H. (2015). Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium. Journal of Visualized Experiments, (102), e53030–e53030. http://doi.org/10.3791/53030
Bogner, B., Boye, S. L., Min, S. H., Peterson, J. J., Ruan, Q., Zhang, Z., … Boye, S. E. (2015). Capsid Mutated Adeno-Associated Virus Delivered to the Anterior Chamber Results in Efficient Transduction of Trabecular Meshwork in Mouse and Rat. PLOS ONE, 10(6), e0128759. https://doi.org/10.1371/journal.pone.0128759
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先谢了
数据分析的小白一枚，最近开始学习二代测序仪IONTORRENT出来的靶向基因检测位点的数据分析。由于完全没有涉猎过这个领域，会有很多不懂得地方，望大神们不吝赐教！
比如上面这个搜索结果，就是根据一起出来的数据，也就是一个位点的rs号进行检索出来的界面，但是完全不知道如何下手，也不是很清楚具体模块的作用，我能得到什么结果。虽然在网上能看到一些说明，但是仍然一知半解的！
最后，还是想说，大神们快快带我入门吧，非常迫切！跪谢！
在非变性聚丙烯酰胺凝胶电泳时，它们的迁移率各不相同，从而获得单倍型特异的电泳带格局即PCR指纹。也有人用人工合成的短寡核苷酸片段作为探针，同经过酶切的人体DNA作Southern blot，可以得出长度不等的杂交带，杂交带的数目和分子量的大小具有个体特异性，除非同卵双生，几乎没有两个人是完全相同的，就象人的指纹一样，人们把这种杂交带图形称为基因指纹(gene finger-printing)。
基因芯片法：又称为DNA 微探针阵列（Micro array）。它是集成了大量的密集排列的大量已知的序列探针，通过与被标记的若干靶核酸序列互补匹配，与芯片特定位点上的探针杂交，利用基因芯片杂交图象，确定杂交探针的位置，便可根据碱基互补匹配的原理确定靶基因的序列。这一技术已用于基因多态性的检测。对多态性和突变检测型基因芯片采用多色荧光探针杂交技术可以大大提高芯片的准确性、定量及检测范围。应用高密度基因芯片检测单碱基多态性，为分析SNPs提供了便捷的方法。
亲子鉴定？
ID,promega.我们实验室主要用的是这两种，由于怕有突变还会备有凯杰的。
如果测序的话，那么就是AB的bigdye吧？
有什么问题我们可以交流。
