- Letrozole
- Aminoglutethimide
- Exemestane
- Formestane
| AnastrozoleAromatase inhibitor |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
Nature.2018 Nov;563(7731):407-411.Nature.2018 Jun 13.Nature.2018 Jun 27.Nature.2018 Mar 29;555(7698):673-677.Nature.2017 Sep 7;549(7670):96-100.Nature.2016 Apr 21;532(7599):398-401.
Science.2016 Aug 5;353(6299)594-8Nat Nanotechnol.2017 Dec;12(12):1190-1198.Nature Biotechnology.2017 Jun;35(6):569-576Nat Med.2018 Sep 17.Cell.2018 Dec 21. pii: S0092-8674(18)31561-7.Cell.Available online 25 October 2018.Cell.2018 Sep 27. pii: S0092-8674(18)31183-8.Cell.2018 Jun 28;174(1):172-186.e21.Cell.2018 Feb 22;172(5):1007-1021.e17.Cell.2017 Nov 30;171(6):1284-1300.e21.Cell.2017 Aug 17. pii: S0092-8674(17)30869-3.Cell.2017 Jul 13;170(2):312-323Nat Med.2018 Jan 29.Nat Med.2017 Nov;23(11):1342-1351.Cell.2017 Apr 6;169(2):286-300.Cell.2015 Aug 27;162(5):987-1002.Cell.2015 Feb 12;160(4):729-44.Nature Medicine.2017 Apr;23(4):493-500.Cancer Cell.2018 May 14;33(5):905-921.e5.Cancer Cell.2018 Apr 9;33(4):752-769.e8.Cancer Cell.2018 Mar 12;33(3):401-416.e8.Cancer Cell.2017 Aug 14;32(2):253-267.e5.Nat Methods.2018 Jul;15(7):523-526.Cell Stem Cell.2018 May 3;22(5):769-778.e4.Cell Stem Cell.2017 Nov 20. pii: S1934-5909(17)30375-2.Quality Control & MSDS
- View current batch:
- Purity = 98.93%
- COA (Certificate Of Analysis)
- HPLC(Retest)
- NMR (Nuclear Magnetic Resonance)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
| Cell experiment [1]: | |
Cell lines | MCF7, HepG2, and PC3 cell lines |
Preparation method | The solubility of this compound in DMSO is >14.2mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reacting condition | 25-400 μg/mL, 24h |
Applications | In MCF7, HepG2, and PC3 cell lines, Anastrozole at 400 μg/mL showed the most significant cytotoxic toward all cell lines. Anastrozole at 400 μg/mL exhibited inhibition rates of 58.4%, 41.7% and 26.6% on MCF7, HepG2, and PC3 cell lines, respectively. In MCF7 breast cancer cells, Anastrozole (200 μg/mL) increased nuclear intensity corresponding to apoptotic changes by 38% and increased cell membrane permeability by 17.3%. Anastrozole also significantly increased cytochrome c release. |
| Animal experiment [2]: | |
Animal models | adult female rats; mature male pigtailed monkeys (M. nernestrina) |
Dosage form | Rats: 0.01-0.1 mg/kg, p.o., on day 2 at 16.00 h or day 3 at 12.00 hMonkeys: 0.003, 0.01, 0.03, 0.1, 0.3 and 1.0 mg/kg, p.o., twice daily (09.00 h and 16.00 h) |
Application | In adult female rats, Anastrozole (0.1 mg/kg) given on day 2 or day 3 completely blocked ovulation. In male pigtailed monkeys, Anastrozole (0.1 mg/kg and above) reduced circulating oestradiol concentrations by 50-60%. The clearance half-life of anastrozole in the monkey was about 7h. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1]. Hassan F1, El-Hiti GA, Abd-Allateef M, et al. Cytotoxicity anticancer activities of anastrozole against breast, liver hepatocellular, and prostate cancer cells. Saudi Med J. 2017 Apr;38(4):359-365. [2] Dukes M1, Edwards PN, Large M, Smith IK, Boyle T. The preclinical pharmacology of "Arimidex" (anastrozole; ZD1033)--a potent, selective aromatase inhibitor. J Steroid Biochem Mol Biol. 1996 Jul;58(4):439-45. | |
Anastrozole Dilution Calculator
calculate
Anastrozole Molarity Calculator
calculate
| Cas No. | 120511-73-1 | SDF | Download SDF |
| Synonyms | N/A | ||
| Chemical Name | 2-[3-(2-cyanopropan-2-yl)-5-(1,2,4-triazol-1-ylmethyl)phenyl]-2-methylpropanenitrile | ||
| Canonical SMILES | CC(C)(C#N)C1=CC(=CC(=C1)CN2C=NC=N2)C(C)(C)C#N | ||
| Formula | C17H19N5 | M.Wt | 293.37 |
| Solubility | ≥14.15mg/mL in DMSO | Storage | Store at -20°C |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Anastrozole (Arimidex,ZD1033) is a potent and selective inhibitor of aromatase with an IC50 value of 14.6 nM or 0.0043μg/ml [1].
Anastrozole has been reported to inhibit human placental aromatase with an IC50 value of 14.6 nM or 0.0043μg/ml. In addition, an oral concentration of 0.1mg/kg of anastrozole has been revealed to completely inhibit ovulation by given on day 2 or day 3 of the cycle. In immature rat, an oral concentration of 0.1mg/kg of anastrozole has also noted to completely extinguish the uterotrophic activity of exogenous AD. Apart from these, by guinea pig, dog and cow adrenal microsomes, anastrozole has been exhibited to suppress the conversion of 11-deoxycortisol to cortisol with mean IC50 values of 4.09μM,129μM and 11.9μM, respectively [1].
References:[1] Dukes M1, Edwards PN, Large M, Smith IK, Boyle T. The preclinical pharmacology of "Arimidex" (anastrozole; ZD1033)--a potent, selective aromatase inhibitor. J Steroid Biochem Mol Biol. 1996 Jul;58(4):439-45.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
先谢了
数据分析的小白一枚，最近开始学习二代测序仪IONTORRENT出来的靶向基因检测位点的数据分析。由于完全没有涉猎过这个领域，会有很多不懂得地方，望大神们不吝赐教！
比如上面这个搜索结果，就是根据一起出来的数据，也就是一个位点的rs号进行检索出来的界面，但是完全不知道如何下手，也不是很清楚具体模块的作用，我能得到什么结果。虽然在网上能看到一些说明，但是仍然一知半解的！
最后，还是想说，大神们快快带我入门吧，非常迫切！跪谢！
在非变性聚丙烯酰胺凝胶电泳时，它们的迁移率各不相同，从而获得单倍型特异的电泳带格局即PCR指纹。也有人用人工合成的短寡核苷酸片段作为探针，同经过酶切的人体DNA作Southern blot，可以得出长度不等的杂交带，杂交带的数目和分子量的大小具有个体特异性，除非同卵双生，几乎没有两个人是完全相同的，就象人的指纹一样，人们把这种杂交带图形称为基因指纹(gene finger-printing)。
基因芯片法：又称为DNA 微探针阵列（Micro array）。它是集成了大量的密集排列的大量已知的序列探针，通过与被标记的若干靶核酸序列互补匹配，与芯片特定位点上的探针杂交，利用基因芯片杂交图象，确定杂交探针的位置，便可根据碱基互补匹配的原理确定靶基因的序列。这一技术已用于基因多态性的检测。对多态性和突变检测型基因芯片采用多色荧光探针杂交技术可以大大提高芯片的准确性、定量及检测范围。应用高密度基因芯片检测单碱基多态性，为分析SNPs提供了便捷的方法。
亲子鉴定？
ID,promega.我们实验室主要用的是这两种，由于怕有突变还会备有凯杰的。
如果测序的话，那么就是AB的bigdye吧？
有什么问题我们可以交流。
