Titanium Taq SP DNA Polymerase is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing. TitaniumTaqSP contains the same Titanium Taq DNA Polymerase and TaqStart Antibody as our standard TitaniumTaqproducts, but with a modified, license-free buffer formulation. Titanium Taq SP performs similarly to our standard Titanium Taq in all tested customer applications. It is ideal for PCR amplification of most DNA templates, and for complex genomic DNA in particular.
Titanium Taq SP DNA Polymerase is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing. TitaniumTaqSP contains the same Titanium Taq DNA Polymerase and TaqStart Antibody as our standard TitaniumTaqproducts, but with a modified, license-free buffer formulation. Titanium Taq SP performs similarly to our standard Titanium Taq in all tested customer applications. It is ideal for PCR amplification of most DNA templates, and for complex genomic DNA in particular.
Titanium Taq is more robust than wild-type Taqpolymerases and allows the amplification of highly complex DNA mixes due to the absence of 5"-exonuclease activity found in wild-typeTaq. This novel enzyme contains carefully engineered amino acid substitutions that increase its solubility, making it incredibly sensitive.
Commercial Use of Titanium Taq SP may provide cost-savings on royalty and license fees in some situations. If you are interested in Commercial Use of our products, including OEM, please contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
If you are purchasing this polymerase for research use only, please visit our standard Titanium Taq DNA Polymerase product page.
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先谢了
2 单细胞测序是指单个细胞水平上对基因组进行测序。
3、靶向测序步骤为 样品准备、探针/引物设计、目标序列捕获、文库制备、上机测序、数据分析。
4、单细胞的分离--DNA/RNA的提取和扩增（全基因组扩增和全转录组扩增）---测序以及后续的分析和应用。
相同:使用二代测序
不同：样本处理，文库构建，检测的区域，数据分析流程
2．pgem-3zf (+) 双链dna对照模板 0.2g/l，试剂盒配套试剂。
3．m13(-21)引物 tgtaaaacgacggccagt，3.2μmol/l，即3.2pmol/μl，试剂盒配套试剂。
4．dna测序模板 可以是pcr产物、单链dna和质粒dna等。模板浓度应调整在pcr反应时取量1μl为宜。本实验测定的质粒dna，浓度为0.2g/l，即200ng/μl。
5．引物 需根据所要测定的dna片段设计正向或反向引物，配制成3.2μmol/l，即3.2pmol/μl。如重组质粒中含通用引物序列也可用通用引物，如m13(-21)引物，t7引物等。
6．灭菌去离子水或三蒸水。
7．0.2ml或和0.5ml的pcr管 盖体分离，pe公司产品。
8．3mol/l 醋酸钠(ph5.2) 称取40.8g naac·3h2o溶于70ml蒸馏水中，冰醋酸调ph至5.2，定容至100ml，高压灭菌后分装。
9．70%乙醇和无水乙醇。
10．naac/乙醇混合液 取37.5ml无水乙醇和2.5ml 3mol/l naac混匀，室温可保存1年。
11．pop 6测序胶 abi产品。
12．模板抑制试剂(tsr) abi产品。
13．10×电泳缓冲液 abi产品。
14．abi prism 310型全自动dna测序仪。
15．2400型或9600型pcr仪。
16．台式冷冻高速离心机。
17．台式高速离心机或袖珍离心机。向左转|向右转
