EpiScope Methylated HeLa gDNA is genomic DNA that has been purified from human HeLa cells and highly methylated using CpG methylase. EpiScope Methylated HCT116 gDNA is genomic DNA purified from human HCT116 cells that is highly methylated using CpG methylase. Both are used as positive controls for DNA methylation analysis methods such as bisulfite sequencing, Combined Bisulfite Restriction Analysis (COBRA), the Methylated CpG Island Recovery Assay (MIRA), and methylation-specific PCR (MSP). Episcope Unmethylated HCT116 DKO gDNA is genomic DNA purified from the DNMT DKO (double knockout) human HCT116 cell line, which is genetically lacking in both DNMT1 (DNA methyltransferase 1) and DNMT3B (DNA methytransferase 3B). The methylation level of genomic DNA from the DNMT DKO HCT116 cell line is much lower (under 5%) than wild-type genomic DNA. Therefore, this DNA can be used as a negative (un- or hypo-methylated) control in DNA methylation analyses including bisulfite sequencing, COBRA, MIRA, and MSP.
EpiScope Methylated HeLa gDNA is genomic DNA that has been purified from human HeLa cells and highly methylated using CpG methylase. EpiScope Methylated HCT116 gDNA is genomic DNA purified from human HCT116 cells that is highly methylated using CpG methylase. Both are used as positive controls for DNA methylation analysis methods such as bisulfite sequencing, Combined Bisulfite Restriction Analysis (COBRA), the Methylated CpG Island Recovery Assay (MIRA), and methylation-specific PCR (MSP). Episcope Unmethylated HCT116 DKO gDNA is genomic DNA purified from the DNMT DKO (double knockout) human HCT116 cell line, which is genetically lacking in both DNMT1 (DNA methyltransferase 1) and DNMT3B (DNA methytransferase 3B). The methylation level of genomic DNA from the DNMT DKO HCT116 cell line is much lower (under 5%) than wild-type genomic DNA. Therefore, this DNA can be used as a negative (un- or hypo-methylated) control in DNA methylation analyses including bisulfite sequencing, COBRA, MIRA, and MSP.
DNA methylation analysis is a field of epigenetics research that has been gaining attention in recent years. Methylation of CpG islands in the promoter region of a gene may influence that gene"s transcriptional regulation. Bisulfite sequencing, methylation-specific PCR (MSP), Combined Bisulfite Restriction Analysis (COBRA) and Methylated CpG Island Recovery Assay (MIRA) are all commonly used to analyze methylated DNA.
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先谢了
数据分析的小白一枚，最近开始学习二代测序仪IONTORRENT出来的靶向基因检测位点的数据分析。由于完全没有涉猎过这个领域，会有很多不懂得地方，望大神们不吝赐教！
比如上面这个搜索结果，就是根据一起出来的数据，也就是一个位点的rs号进行检索出来的界面，但是完全不知道如何下手，也不是很清楚具体模块的作用，我能得到什么结果。虽然在网上能看到一些说明，但是仍然一知半解的！
最后，还是想说，大神们快快带我入门吧，非常迫切！跪谢！
在非变性聚丙烯酰胺凝胶电泳时，它们的迁移率各不相同，从而获得单倍型特异的电泳带格局即PCR指纹。也有人用人工合成的短寡核苷酸片段作为探针，同经过酶切的人体DNA作Southern blot，可以得出长度不等的杂交带，杂交带的数目和分子量的大小具有个体特异性，除非同卵双生，几乎没有两个人是完全相同的，就象人的指纹一样，人们把这种杂交带图形称为基因指纹(gene finger-printing)。
基因芯片法：又称为DNA 微探针阵列（Micro array）。它是集成了大量的密集排列的大量已知的序列探针，通过与被标记的若干靶核酸序列互补匹配，与芯片特定位点上的探针杂交，利用基因芯片杂交图象，确定杂交探针的位置，便可根据碱基互补匹配的原理确定靶基因的序列。这一技术已用于基因多态性的检测。对多态性和突变检测型基因芯片采用多色荧光探针杂交技术可以大大提高芯片的准确性、定量及检测范围。应用高密度基因芯片检测单碱基多态性，为分析SNPs提供了便捷的方法。
亲子鉴定？
ID,promega.我们实验室主要用的是这两种，由于怕有突变还会备有凯杰的。
如果测序的话，那么就是AB的bigdye吧？
有什么问题我们可以交流。
