General Category:
- Monoclonal antibodies » Cardiac Markers » Other Cardiac Markers
Disease Category:
- Cardiac Markers » Monoclonal antibodies » Other Cardiac Markers
New MAbs: 1H4, 2A3, 2B10, 10A6Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells
of Balb/c mice immunized with recombinant human soluble CD40 Ligand / TRAP Analog
conjugates with BSA.
IgG1 for all MAbs
Human sCD40L immunoassay. All MAbs work in enzyme immunoassays. MAbs can be used for
detection of human sCD40L in serum.
All MAbs work in Western blotting, but for best sensitivity we recommend MAbs 2B10 and 30B4.
Recommended pairs for sandwich immunoassay for detection of native sCD40L in human serum
(capture-detection):
1H4 – 2A3
10A6 – 2A3
30B4 – 2A3
Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small
appropriate care must be taken when handling this product.
See references where Soluble CD40 ligand has been used in.
ebiomall.com
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般基克隆物体直接取基包括面几步骤
提取植物或者物基组DNA(试剂盒)————做PCR需要基扩增(顺利2搞定)——扩增基做酶切——基连接想插入载体面(顺利1-2)——鉴定克隆基
步骤都商品化试剂或者试剂盒 要思路清晰做起快 1-2周能完
没证实情况任何没依据推断都假想空想
现在小弟碰见一个难题,我想克隆一个基因,但是我在NCBI一检索,发现别人只发表了这个基因的DNA全长,但是,CDNA没有发表,也没有发表文章,想请问下,在不知道CDNA的情况下,请问我应该如何做这个基因的克隆???(全基因组也不知道,其他生物没有这种基因)
详见:http://zhidao.baidu.com/question/1831844001436785260.html?fr=iks&word=%B2%A1%B6%BE%B5%C4%B8%B4%D6%C6%D6%DC%C6%DA%B5%C4%B9%FD%B3%CC%3F&ie=gbk

