General Category:
- Monoclonal antibodies » HormonesMonoclonal antibodies » Veterinary » Other animal specific antibodies
Disease Category:
- Hormones » Monoclonal antibodiesVeterinary » Monoclonal antibodies » Other animal specific antibodies
Hybridoma clone for MAb XM207 has been derived from hybridization of Sp2/0 myeloma cells with
spleen cells of Balb/c mice immunized with conjugate of purified progesterone with BSA. For
HPRO- 1, HPRO-2 and HPRO-3 immunogen was conjugate of 17⍺-hydroxyprogesterone with BSA.
MAb HPRO-2: Progesterone
MAbs HPRO-1, HPRO-3: 17⍺-hydroxyprogesterone
MAb XM207 reacts with progesterone in ELISA.
MAbs HPRO-1 and HPRO-3 react with 17⍺-hydroxyprogesterone-BSA conjugate and free 17α-
hydroxyprogesterone. There is no cross-reactivity with BSA.
IgG2b for MAbs XM207, HPRO-2 and HPRO-3
IgG3 for MAb HPRO-1
HPRO-1 and HPRO-3 can be used in competitive assay of 17⍺-hydroxyprogesterone.
exchange chromatography.
NaCl, 10 mM Tris, pH 7.5, 0.05% of sodium azide (NaN3).
Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small
appropriate care must be taken when handling this product.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
般基克隆物体直接取基包括面几步骤
提取植物或者物基组DNA(试剂盒)————做PCR需要基扩增(顺利2搞定)——扩增基做酶切——基连接想插入载体面(顺利1-2)——鉴定克隆基
步骤都商品化试剂或者试剂盒 要思路清晰做起快 1-2周能完
没证实情况任何没依据推断都假想空想
现在小弟碰见一个难题,我想克隆一个基因,但是我在NCBI一检索,发现别人只发表了这个基因的DNA全长,但是,CDNA没有发表,也没有发表文章,想请问下,在不知道CDNA的情况下,请问我应该如何做这个基因的克隆???(全基因组也不知道,其他生物没有这种基因)
详见:http://zhidao.baidu.com/question/1831844001436785260.html?fr=iks&word=%B2%A1%B6%BE%B5%C4%B8%B4%D6%C6%D6%DC%C6%DA%B5%C4%B9%FD%B3%CC%3F&ie=gbk

