PrimeScript Reverse Transcriptase has exceptionally strong strand-displacement activity and enables efficient preparation of cDNA up to 12 kb in length. It is robust, versatile, and well-suited for applications requiring full-length cDNA, such as preparation of cDNA libraries and other techniques involving first-strand cDNA synthesis (RT-PCR, preparation of cDNA probes, real-time quantitative RT-PCR).
PrimeScript Reverse Transcriptase has exceptionally strong strand-displacement activity and enables efficient preparation of cDNA up to 12 kb in length. It is robust, versatile, and well-suited for applications requiring full-length cDNA, such as preparation of cDNA libraries and other techniques involving first-strand cDNA synthesis (RT-PCR, preparation of cDNA probes, real-time quantitative RT-PCR).
PrimeScript RT can be used to perform reverse transcription from any RNA template, including GC-rich RNAs and those with high levels of secondary structure. This enzyme is a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase and is verified to be RNase H-minus. Because of the excellent extension capability of PrimeScript RT, preparation of cDNAcan be performed at a lower temperature (42°C), decreasing the risk of RNA degradation that can occur during conventional reactions performed at higher temperatures.
First introduced in Japan, PrimeScript is now available to scientists worldwide. Over 3,700 peer-reviewed articles have been published in which PrimeScript Reverse Transcriptase was referenced in a variety of scientific applications such as gene expression profiling, gene discovery, transcriptome analysis, miRNA expression and regulation analysis, molecular evolution studies, virology, and microbiology.
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般基克隆物体直接取基包括面几步骤
提取植物或者物基组DNA（试剂盒）————做PCR需要基扩增（顺利2搞定）——扩增基做酶切——基连接想插入载体面（顺利1-2）——鉴定克隆基
步骤都商品化试剂或者试剂盒 要思路清晰做起快 1-2周能完
没证实情况任何没依据推断都假想空想
现在小弟碰见一个难题，我想克隆一个基因，但是我在NCBI一检索，发现别人只发表了这个基因的DNA全长，但是，CDNA没有发表，也没有发表文章，想请问下，在不知道CDNA的情况下，请问我应该如何做这个基因的克隆？？？（全基因组也不知道,其他生物没有这种基因）
详见：http://zhidao.baidu.com/question/1831844001436785260.html?fr=iks&word=%B2%A1%B6%BE%B5%C4%B8%B4%D6%C6%D6%DC%C6%DA%B5%C4%B9%FD%B3%CC%3F&ie=gbk
