Youneedtobuyglassslideswithfrosted,sandblastedends(FisherScientific,CatalogN.12-552).Frostingbypainting(e.g.Superfrost)shouldnotbeused.Pre-cleantheslidesbyrubbingtheedgesoftheslidesagainsteachotherinmedium,togetridoftheglasspowder.Slidescanbeautoclavedinadvanceforsteriledissociation. Placeapieceofspleenorawholespleencutin2or3piecesbetweenthefrostedendsoftwoglassslides.Gentlypressand,withacircularmotion,dissociatethetissuebetweentheslidesuntilissatisfactory. clickontheimagetoenlarge Thymus,lymphnodes,Peyer"sPatchesandfetallivercanbedissociatedwiththismethod.Cellswithintercellularjunctionswilldisintegrateafterdissociationwiththismethod. Filterwithafinemeshnylonfilter.CountviABIlity.