- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Recognizes δ unsaturated disaccharide of unsulfated chondroitin generated by chondroitinase ABC digestion.
Data/Specifications
Immunogen: Bovine nasal cartilage aggrecan that had been deglycosylated with chondroitinase ABCdigestion; i.e. BNC (ABC) Core (see Couchman et al 1984 and Caterson et al 1985).
Clone: 1B5
Concentration: 0.1 mg/mL
Isotype: IgG1K
Host: Mouse
Purity: Protein A
Specificity: This antibody recognizes zero sulphated Chondroitin Sulphate stub neoepitope generated by chondroitinase ABC treatment.
Form: Clear liquid; PBS, no preservatives
Storage: -20° C
Literature/Support
Chondroitin Sulphate Neoepitope Antibody (Insert)
A.J. Hayes et al. (2008) Methods 45:10–21
Couchman JR, Caterson B, Christner JB, Baker JR (1984) Mapping by monoclonal antibody detection of glycosaminoglycans in connective tissues. Nature 307:650–652
Caterson B, Christner JE, Baker JR, Couchman JR (1985) Production and characterization of monoclonal antibodies directed against connective tissue proteoglycans. Fed Proc 44:386–393
Anthony J. Hayes, Amanda Hall, Liesbeth Brown, Ross Tubo, and Bruce Caterson (2007) Macromolecular Organization and In Vitro Growth Characteristics of Scaffold-free Neocartilage GraftsJournal of Histochemistry & Cytochemistry Volume 55(8): 853–866
How To Use
Applications:
- Western Blot: use at dilution of 1/100.
- Immunohistochemistry: Formalin or paraformaldehyde-fixed paraffin embedded sections, alcohol-fixed frozen sections or un-fixed snap-frozen sections.
Technical Notes:
Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.
Related Products
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异：金属的升温和降温比较快，比热容小；水浴升温和降温慢，等待时间长
过完年，再开培养箱参数不稳定，两个箱子，co2数值一直在闪～我要养细胞，参数设置的是37℃，5%CO2,请问各位大神有知道咋回事的～还有面板上各种符号啥意思？图中标记的都是在闪的参数
想用96孔板，酶标仪来测定，一来量大，二来试剂用量小，
可是遇到一个问题，在使用说明书中要37度水浴5分钟，
请问大家我怎么96孔板水浴啊，先谢谢各位了！！
有什么其他方法来代替水浴啊～！
2，适宜的床面横向坡度。
3，冲洗水大小要适宜。
4，给矿量适当切均匀。
5，给矿浓度适宜。
6，物料在入选前的准备。
7，物料在床面的分带和产品的截取。
操作的时候还有一些细节，有什么问题可以咨询河南红星机器，摇床生产出口基地，30年的生产经验，可以免费安装调试维修。
如图，有谁用过这种高压蒸汽灭菌锅，“TIME”指示灯为啥没有显示? 有点搞不懂。
文献上说是液氮保存，
实验室现在只有－80度低温冰箱，可不可以代替呢？
