Product Specifications:
Item# 1004 : Recombinant HIV-1 IIIB Glycoprotein Soluble gp160
Concentration: See Vial
Mass/Vial: 100µg
Diluent: PBS In-Vitro Diagnostics
Purity: >95%
Stabilizer: None
Preservative: None
Storage: -75°C
Physical State: Frozen Liquid
Stability: 2 years at -75°C.
Application: CD4 Binding, Vaccines, T- Cell Activation, In- Vitro Diagonostics.
Description: Full length Recombinant HIV-1 IIIB soluble - membrane anchor sequence deleted- gp160 glycoprotein produced in the Baculovirus Expression System.
Purification: This protein is purified by immunoaffinity Chromatography to >95% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificit and human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp160/Anti gp160 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.
Applications and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. HIV-1 converted human serum polyclonal antibodies yield titers of 1:1000 or greater at 1-10ng of immobilized protein under standard ELISA conditions.
Item#: 1004-P Peroxidase-Conjugated rgp160 HIV-1 IIIB
Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 studies.
Item#: 1004-G Colloidal gold-Conjugated rgp160 HIV-1 IIIB
Application: Human diagnostics, CD4 binding, Drug screening, FACS analysis.
Item#: 1004-F Flourescene-Conjugated rgp160 HIV-1 III
Application: FACS analysis, Histocytology.
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为了提高活体细胞的显微图像分辨率虽然有多种方法,然而至今还未找到一种公认的处理所有活体细胞图像方法。本文采用伪彩色处理与多种图像处理方法结合处理50幅原始图像,并比较处理前后的卵母细胞图像 ,使用SPSS10.0统计软件包加以处理 ,探讨处理活体细胞是否能提高图像的分辨率及系统是否适用于活体细胞的图像处理 ,为今后临床和科研研究活体细胞创造条件。
我想除了PCR方面有些问题外，成像仪的使用和调校我也不是很熟悉,请问各位老师，怎么才能使背景变黑,不至于胶都看得清楚呢?我试过调亮度,GAMMA,但是效果不好.
请教各位,使用成像仪的心得,谢谢了
