| Source | human 293 cells | M.W. | ~180 kDa | CAS No. | |
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in a sterile solution of PBS. | ||||
| Reconstitution | Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized peptide shouldbe reconstituted in sterile water. | ||||
| Stability | The lyophilized protein isstable for at least 1 year if stored at -80°C. Reconstituted protein is stable for at least 1 month at 4 °C, but shouldbe stored in aliquots at -80°C for longer term. Avoid repeated freeze andthaw. | ||||
| Purity | Greater than 90% as determined by SDS-PAGE analysis | ||||
| Biological Activity | Determined by a Notch pathway specific reporter gene assay.EC50s are in the range of 200 – 1000 ng/mL. | ||||
| Country of Origin | USA | ||||
The Notch pathwayis an important pathway in stem cell functions.Jagged-1, a ligand for Notch receptors, is a 180kDa type 1 membraneglycoprotein of the Delta/Serrate/Lag2 (DSL) family.StemRD’s Jagged-1 Fcfusion protein is expressed in human 293 cells as the 1046aa N-terminalextracellular domain of Jagged-1 fused to the Fc domain of human IgG1. This fusion stabilizes Jagged-1 in vitro andin vivo without significant compromise in its biological activity.
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交联实验验证细胞中两个蛋白之间的相互作用,交联剂应用之后,电泳之前样本要怎样处理?是否需要超声破碎仪处理细胞?超声处理会不会破坏蛋白二聚体?
第一、在第一次使用时,先对照仪器说明书检查仪器所带配件是否齐全,譬如搅拌子、电源线等;
第二、调速时应由低速逐步调至高速,最好不要高速档直接起动,以免搅拌子不同步,引起跳动;
第三、中速运转可延长搅拌器的使用寿命;
第四、仪器应保持清洁干燥,尤其不要使溶液进入机内;
第五、搅拌时如果发现搅拌子跳动或不搅拌,请检查一下烧杯是否平稳,位置是否正;
第六、不搅拌时不能加热,不工作时应切断电源;
第七、使用时最好能够接上地线。
参考资料:Hi.baidu/=。www.jingda17.cn。DI
溶解后加培养液稀释不会出现悬浮浑浊吗??????
1.我利用氯仿萃取生物碱,需要将氯仿蒸干,我做的样品有非常多份,但是每份样品很少,用的氯仿也很少,每份只用十几毫升左右,但是旋转蒸发仪的蒸发瓶好大,而且一次只能蒸发一个样,太慢了,就算蒸发完了,取出生物碱的时候也不好取,由于我做的是定量试验,所以如果取出生物碱时有残留会影响实验结果,到底怎么办啊?有没有特制的小的蒸发瓶?或者特制的可以一次接好几个蒸发容器的接口?
2.请问恒温水浴锅可不可以用来做蒸发?由于是蒸发氯仿,担心没有回收装置会导致泄露中毒的问题,但是我的量比较少,又想冒险试试,真是焦头烂额了现在,大家把那个我出出主意吧!!!!

