
Source | human 293 cells | M.W. | ~180 kDa | CAS No. | |
---|---|---|---|---|---|
Structural Info | |||||
Formulation | Lyophilized in a sterile solution of PBS. | ||||
Reconstitution | Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized peptide shouldbe reconstituted in sterile water. | ||||
Stability | The lyophilized protein isstable for at least 1 year if stored at -80°C. Reconstituted protein is stable for at least 1 month at 4 °C, but shouldbe stored in aliquots at -80°C for longer term. Avoid repeated freeze andthaw. | ||||
Purity | Greater than 90% as determined by SDS-PAGE analysis | ||||
Biological Activity | Determined by a Notch pathway specific reporter gene assay.EC50s are in the range of 200 – 1000 ng/mL. | ||||
Country of Origin | USA |
The Notch pathwayis an important pathway in stem cell functions.Jagged-1, a ligand for Notch receptors, is a 180kDa type 1 membraneglycoprotein of the Delta/Serrate/Lag2 (DSL) family.StemRD’s Jagged-1 Fcfusion protein is expressed in human 293 cells as the 1046aa N-terminalextracellular domain of Jagged-1 fused to the Fc domain of human IgG1. This fusion stabilizes Jagged-1 in vitro andin vivo without significant compromise in its biological activity.
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产品名称:磁力搅拌器ST5ST10
产品品牌:韩国FINEPCR
产品类别:磁力搅拌器
产品详细参数
外形小巧,性能卓越
不带加热的多点磁力搅拌器
高品质的无碳刷电机可提供安静而强有力的搅拌
搅拌转速从0—1300rpm可调
不锈钢表台可防止液体喷溅
外观小巧,操作安全简便
性能参数:
型号
ST5
ST10
转速范围
0—1300rpm
搅拌点间距离
90mm
容量
单点搅拌:2L
250ml*5
250ml*10
材质
表台:不锈钢
重量
3Kg
5Kg
尺寸大小(W*D*H)
114*564*63mm
197*564*63mm
功率
15W
30W
电源
110V/220V/230V,50/60Hz
更多详情请登录:http://www.sci-bridge.com/ProductsList.aspx?pid=186
是不是可以先放-70冰箱预冷,然后就可以加液氮研磨组织了?
会不会爆裂啊?
加trizol,之后,还需要用玻璃匀浆器磨么?
个人觉得没有必要,如果需要的话是为什么呢?是怕组织没有完全破碎么?
谢谢大家了!
动物组织块冻存在液氮,取出后放到1.5mlEP管中,EP管中加入RIPA裂解液(按照0.1mg组织最多1ml裂解液的体积使用,用前已加入PMSF),使用电动组织研磨器研磨,每次研磨时间10秒左右,研磨过程中发现蛋白质起泡,就像做蛋糕时鸡蛋被打出泡一样。请问如何避免这种情况?
PS,本来试着在冰上操作,但是电动组织研磨器工作时EP管震动的很厉害,没法放在冰上。
主机型号:Biosafer950E
工作频率范围:20-25KHz频率自动跟踪
功率调节范围:2-99%连续可调
工作时间:0-999(s/m/h/t)
间隙时间:0.1-99.9s
工作计时方式:正序/倒序
温度保护设定:有
超声时间:0.1-99.9s
功率振幅显示:有
工作模式:间隙/连续/手动模式
通讯接口:RS485 RS232 PC端软件
功率:950W
标配变幅杆:Ф6mm
破碎量:0.5-600ml可选配变幅标:Ф2、Ф3、Ф10

