Human CD14 is strongly expressed on myelomonocyte lineage cells including monocytes, macrophages, and a subset of granulocytes. CD14 is a high affinity receptor for complexes of lipopolysaccharide and lipopolysaccharide binding protein. A soluble form of CD14 also binds to LPS and LBP (5).
Isotype: Murine IgG2a
Immunogen: Human thymocytes/Sezary T cells
Specificity: Antibody UCHM1 recognizes an extracellular epiotpe of the human CD14 molecule of about 53 kd. UCHM1 will also immunoprecipitate a soluble form of CD14 (1, 2, 3). It is crossreactive with cynomolgus monkey CD14 (6).
References:
1. N. Hogg, et al, (1984) Immunology 53: 753-767.
2. M.O. Labeta, et al, (1993) Eur J Immunol 23: 2144-2151.
3. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989)”Reference Antibody”.
4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 778-788.
5. S.D. Wright, (1995) J Immunol 155: 6-8.
6. Yoshino N, Honda M, et al. (2000) Exp Anim 49(2):97-110.
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PCR技术类似于DNA的天然复制过程,其特异性依赖于与靶序列两端互补的寡核苷酸引物,由变性--退火--延伸三个基本反应步骤构成
2、仪器功能:进口和国产仪器主要功能:如保存,过温保护,断电保护,热盖功能等基本上是一样的,只是附带功能上有所差别,比如:进口的显示屏是大型的触摸式荧屏操作,国产的大多是触摸键,荧屏显示操作,进口仪器有的可以升级为荧光定量PCR仪,而国产的目前主要是专用机。而这些仪器附带功能的差别大多都是最近两年新推出来的,价格确是相差几倍。有的进口仪器没有这些附带的特殊功能,从性能和功能上比有的等于或低于国产的。
普通PCR的孔所有温度是一样的。

