ELISA Kit for Eosinophil Chemotactic Factor (ECF)
CCL11; SCYA11; Eotaxin 1; Eosinophil Chemotactic Protein 1; Chemokine C-C-Motif Ligand 11; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11
- Product No.SEA025Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.8pg/mL.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 349 For more details, please contact local distributors!US$ 498 For more details, please contact local distributors!US$ 2241 For more details, please contact local distributors!US$ 4233 For more details, please contact local distributors!US$ 34860 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
This assay has high sensitivity and excellent specificity for detection of Eosinophil Chemotactic Factor (ECF).No significant cross-reactivity or interference between Eosinophil Chemotactic Factor (ECF) and analogues was observed.
Recovery of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
Matrices listed below were spiked with certain level of recombinant Eosinophil Chemotactic Factor (ECF) and the recovery rates were calculated by comparing the measured value to the expected amount of Eosinophil Chemotactic Factor (ECF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-102 | 85 |
| EDTA plasma(n=5) | 96-105 | 101 |
| heparin plasma(n=5) | 96-105 | 101 |
Precision of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Eosinophil Chemotactic Factor (ECF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-88% | 95-102% | 97-105% | 93-101% |
| EDTA plasma(n=5) | 87-98% | 99-105% | 93-101% | 83-104% |
| heparin plasma(n=5) | 88-96% | 99-105% | 87-101% | 78-95% |
Stability of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eosinophil Chemotactic Factor (ECF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eosinophil Chemotactic Factor (ECF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eosinophil Chemotactic Factor (ECF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eosinophil Chemotactic Factor (ECF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APA025Hu01 | Active Eosinophil Chemotactic Factor (ECF) | Cell culture; Activity Assays. |
| RPA025Hu01 | Recombinant Eosinophil Chemotactic Factor (ECF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA025Hu02 | Recombinant Eosinophil Chemotactic Factor (ECF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA025Hu01 | Polyclonal Antibody to Eosinophil Chemotactic Factor (ECF) | WB; IHC; ICC; IP. |
| MAA025Hu22 | Monoclonal Antibody to Eosinophil Chemotactic Factor (ECF) | WB; IHC; ICC; IP. |
| SEA025Hu | ELISA Kit for Eosinophil Chemotactic Factor (ECF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA025Hu | CLIA Kit for Eosinophil Chemotactic Factor (ECF) | Chemiluminescent immunoassay for Antigen Detection. |
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谢谢！
做完实验之后放8联排的卡槽怎么按都弹不出来了。求助各位可能的原因有哪些？原因越详细越多越好，避免下次再出现这种状况，有没有其他战友遇到过这种情况？谢谢！
PCR技术类似于DNA的天然复制过程，其特异性依赖于与靶序列两端互补的寡核苷酸引物,由变性--退火--延伸三个基本反应步骤构成
2、放 PCR 离心管，先把顶盖向上扳，再往前推，露出放样孔，PCR 管
放入前应加好反应体系各组分，再放入 PCR 离心管。
3、反向重复第二步骤将顶盖板向后拉，盖好顶盖。
4、按 F2“Create”新建一个扩增的 PCR 程序。
5、按控制台面右方上下左右方向键，可随意移动编辑位置，输入需要
的温度、时间、循环数等。
6、据 PCR 离心管中加入的反应体积总量，在“Reaction volume”后输入
相应数值，有 Std“1～100ul”和 9600“1~50ul”两档可供选择。
7、按 F1“Start”启动
8、按“INFO”对应键查看 PCR 结束键。
9、扩增完成后按台面左方“Stop”键退出。
10、按“Hist”，可查看上次扩增的历史记录。11、完全退出后，按台面左下方电源开关，拔出插销，切断电源。
2、仪器功能：进口和国产仪器主要功能：如保存，过温保护，断电保护，热盖功能等基本上是一样的，只是附带功能上有所差别，比如：进口的显示屏是大型的触摸式荧屏操作，国产的大多是触摸键，荧屏显示操作，进口仪器有的可以升级为荧光定量PCR仪，而国产的目前主要是专用机。而这些仪器附带功能的差别大多都是最近两年新推出来的，价格确是相差几倍。有的进口仪器没有这些附带的特殊功能，从性能和功能上比有的等于或低于国产的。
