Recombinant Human His6-Miro1/Rhot1 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
SP-490
Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Miro1/Rhot1
Mitochondrial Rho GTPase 1 (also known as Miro1 or Rhot1) is a membrane associated GTPase with a predicted molecular weight of 71 kDa. Miro1 has been reported to directly associate with the KIF5 motor protein, allowing mitochondria to move along microtubules. A number of publications have identifed Miro1 as an in vivo substrate for the E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene), an essential component of the cellular machinery that participates in the removal of damaged mitochondria. This protein is useful as an in vitro substrate for activated recombinant Parkin enzyme.
- Birsa, N. et al. (2014) J. Biol. Chem. 289: 14569
- Kazlauskaite, A. et al. (2014) Open Biol. 4: 130213
- Klosowiak, J.L. et al. (2016) Sci. Rep. 6: 33019
- MacAskill, A.F. et al.(2009) Neuron 61: 541
- Ordureau, A. et al. (2015) Proc. Natl. Acad. Sci. 112: 637
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谢谢!
2、仪器功能:进口和国产仪器主要功能:如保存,过温保护,断电保护,热盖功能等基本上是一样的,只是附带功能上有所差别,比如:进口的显示屏是大型的触摸式荧屏操作,国产的大多是触摸键,荧屏显示操作,进口仪器有的可以升级为荧光定量PCR仪,而国产的目前主要是专用机。而这些仪器附带功能的差别大多都是最近两年新推出来的,价格确是相差几倍。有的进口仪器没有这些附带的特殊功能,从性能和功能上比有的等于或低于国产的。
各位大神,有使用过ThermoPX2这个型号的pcr扩增仪吗?
普通PCR的孔所有温度是一样的。