Probe qPCR Mix, with UNG is designed for probe-based qPCR. A combination of a hot-start PCR enzyme and an optimized buffer results in excellent suppression of nonspecific amplification, high amplification efficiency, and high detection sensitivity in real-time PCR analyses. This product is also suitable for high-speed PCR. It allows accurate target quantification and detection over a broad dynamic range and makes it possible to perform highly reproducible and reliable real-time PCR analyses.
Probe qPCR Mix, with UNG is designed for probe-based qPCR. A combination of a hot-start PCR enzyme and an optimized buffer results in excellent suppression of nonspecific amplification, high amplification efficiency, and high detection sensitivity in real-time PCR analyses. This product is also suitable for high-speed PCR. It allows accurate target quantification and detection over a broad dynamic range and makes it possible to perform highly reproducible and reliable real-time PCR analyses.
Probe qPCR Mix, with UNG is supplied as a 2X premix containing Tli RNase H and Uracil N-Glycosylase (UNG). A heat-resistant Tli RNase H can minimize PCR inhibition by degrading residual mRNA in reactions using cDNA templates, while UNG can avoid false positives due to carryover contamination by degrading previously amplified PCR products with this kit.
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普通PCR的孔所有温度是一样的。
谢谢！
PCR技术类似于DNA的天然复制过程，其特异性依赖于与靶序列两端互补的寡核苷酸引物,由变性--退火--延伸三个基本反应步骤构成
各位大神，有使用过ThermoPX2这个型号的pcr扩增仪吗？
