Probe qPCR Mix, with UNG is designed for probe-based qPCR. A combination of a hot-start PCR enzyme and an optimized buffer results in excellent suppression of nonspecific amplification, high amplification efficiency, and high detection sensitivity in real-time PCR analyses. This product is also suitable for high-speed PCR. It allows accurate target quantification and detection over a broad dynamic range and makes it possible to perform highly reproducible and reliable real-time PCR analyses.
Probe qPCR Mix, with UNG is designed for probe-based qPCR. A combination of a hot-start PCR enzyme and an optimized buffer results in excellent suppression of nonspecific amplification, high amplification efficiency, and high detection sensitivity in real-time PCR analyses. This product is also suitable for high-speed PCR. It allows accurate target quantification and detection over a broad dynamic range and makes it possible to perform highly reproducible and reliable real-time PCR analyses.
Probe qPCR Mix, with UNG is supplied as a 2X premix containing Tli RNase H and Uracil N-Glycosylase (UNG). A heat-resistant Tli RNase H can minimize PCR inhibition by degrading residual mRNA in reactions using cDNA templates, while UNG can avoid false positives due to carryover contamination by degrading previously amplified PCR products with this kit.
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PCR技术类似于DNA的天然复制过程，其特异性依赖于与靶序列两端互补的寡核苷酸引物,由变性--退火--延伸三个基本反应步骤构成
2、仪器功能：进口和国产仪器主要功能：如保存，过温保护，断电保护，热盖功能等基本上是一样的，只是附带功能上有所差别，比如：进口的显示屏是大型的触摸式荧屏操作，国产的大多是触摸键，荧屏显示操作，进口仪器有的可以升级为荧光定量PCR仪，而国产的目前主要是专用机。而这些仪器附带功能的差别大多都是最近两年新推出来的，价格确是相差几倍。有的进口仪器没有这些附带的特殊功能，从性能和功能上比有的等于或低于国产的。
普通PCR的孔所有温度是一样的。
