The inducible costimulator CD278 (ICOS) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or CD278 results in discrete cytokine secretion profiles by the activated T cells.1 Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement.2 Human CD275 (ICOSL, GL50, B7-H2) is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for CD278 (ICOS).3 Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain. 4
Isotype: Murine IgG1 Kappa
Immunogen: Human HPB-MLT cells, recombinant human CD278(ICOS)-muIg
Specificity: Antibody ANC6C6 binds to recombinant and cell surface CD278(ICOS)
Functional Application: Antibody ANC6C6 blocks binding of Recombinant CD275-muIg to CD278. It blocks CD28-superagonist/CD275(LICOS) mediated T cell proliferation in vitro(7).
References:
1) Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717.
2) Riley, J.L., C.H. June, et al. 2001, J. Immunol. 166: 4943-4948.
3) Ling, V., M. Collins, et al. 2000, J. Immunol. 164: 1653-1657.
4) Ling, V., M. Collins, et al. 2001, J. Immunol. 166: 7300-7308.
5) Tomoki Ito, Yong-Jun Liu, et al. (2008) Immunity 28: 870-880.
6) Hyung W Lim, Chang H Kim. (2007) J Immunol 179: 7448-7456.
7) S Weissmuller, Z Waibler, et al. (2012) Blood 119(26):6268-6277.
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液氮冷冻的原理与工艺：液氮是一种比较理想的制冷剂，五色透明、稍轻于水、惰性强、无腐蚀、对震动、电火花是稳定的，一个大气压下，液氮的汽化温度为—195.81t，蒸发潜热为47.9kcal/kg，由于空间技术和炼钢等工业的发展，大量的制氧副产品——氮气经过液化得到的液氮作为一种深冷冷源已经广泛应用于医药、激光、超导、食品、生物等各工业生产和科研领域。20世纪肋年代液氮制冷剂直接气化制冷修筑地下建筑工程，已成为一种新的土层冻结方法，为提高地层冻结速度开辟了新的途径。
我现在要测脑组织匀浆的SOD活性和MDA的含量，但是因为还有其他的一些指标要测，所以需要先把脑组织保存５－１０天左右才有时间测．虽然这两个指标有很多人测，我查了一下资料，也问了几个人，答案各不相同，有人说是放在低温冰箱里面就可以了，液氮罐只是适用于长期保存；也有人说放低温冰箱SOD的活性容易丧失，到底该怎么样保存最合适？谢谢！
最好是采集完了之后用液氮速冻后,存放在-70的超低温冰箱里.-20度并不能存多久,尤其是如果你要用来做cDNA文库这类对RNA质量要求很高的实验的话是绝对不行的.
1.先将冻存管放入4℃冰箱,约40min.
2.接着置于-20℃冰箱,约30～60min.
3.置于-80℃超低温冰箱中放置过夜.
4.置于液氮罐中长期保存.
